CAJ | 학술논문

目的观察化疗前后不同时期胶质瘤组织中毛细血管扩张性共济失调突变基因( ATM)表达变化.方法利用人胶质瘤U251细胞裸鼠皮下异位移植制作胶质瘤化疗模型,通过顺铂对荷瘤裸鼠进行化疗(每天5 mg/kg,连续腹腔注射4d),建立胶质瘤化疗和化疗后复发模型.利用功能分类基因芯片系统检测化疗前后不同时期胶质瘤组织中ATM的表达,并利用逆转录-聚合酶链反应(RT-PCR)和免疫组织化学法进行验证.结果 ATM在化疗前后不同时期表达出现明显变化,化疗结束时ATM相对表达量较化疗前明显上调(27.96±14.78比1.00±0.00,P＜0.01),在化疗后肿瘤消退至最小时ATM表达又明显下调(4.40±3.41,P＜0.05),然而在胶质瘤复发时其表达再次明显上调(30.15±7.02,P＜0.01),定量RT-PCR和免疫组织化学结果与此一致.结论化疗前后不同时期胶质瘤组织中ATM的表达呈动态变化并存在时间窗口特征.
목적 관찰화료전후불동시기효질류조직중모세혈관확장성공제실조돌변기인( ATM)표체변화.방법 이용인효질류U251세포라서피하이위이식제작효질류화료모형,통과순박대하류라서진행화료(매천5 mg/kg,련속복강주사4d),건립효질류화료화화료후복발모형.이용공능분류기인심편계통검측화료전후불동시기효질류조직중ATM적표체,병이용역전록-취합매련반응(RT-PCR)화면역조직화학법진행험증.결과 ATM재화료전후불동시기표체출현명현변화,화료결속시ATM상대표체량교화료전명현상조(27.96±14.78비1.00±0.00,P＜0.01),재화료후종류소퇴지최소시ATM표체우명현하조(4.40±3.41,P＜0.05),연이재효질류복발시기표체재차명현상조(30.15±7.02,P＜0.01),정량RT-PCR화면역조직화학결과여차일치.결론 화료전후불동시기효질류조직중ATM적표체정동태변화병존재시간창구특정.
Objective To study the expression of ataxia-telangiectasia mutated (ATM) in preand post-chemotherapeutic glioma.Methods Ectopicly subcutaneous glioma transplantation models in nude mice were established by injection of human glioma cell line U251 cells.Tumor-bearing nude mice were treated by the intraperitoneal injection of cisplatin [ at the dose of 5 mg/( kg· day) for 4 days continuously ],and chemotherapeutic and recurrent glioma models after chemotherapy were established.The expression of ATM in pre- and post-chemotherapeutic glioma was detected by using oligo toxicology & drug resistance microarrays,reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry.Results The expression of ATM was significantly increased in gliomas after chemotherapy (27.96 ±14.78 vs 1.00 ±0.00,P ＜0.01 ),significantly decreased when the size of gliomas was smallest (4.40 ±3.41,P ＜ 0.05 ),and increased obviously again when gliomas relapsed ( 30.15 ± 7.02,P ＜ 0.01 ).RT-PCR and immunohistochemistry revealed the similar results.Conclusion The expression of ATM changes ambulatorily in gliomas pre- and post-chemotherapy and there exists a time window.