CAJ | 학술논문

目的通过荧光偏振免疫测定(fluorescence polarization immunoassay,FPIA)和酶放大免疫测定技术(enzyme-multiplied immunoassay technique,EMIT)测定环孢素A(cyciosporine,CsA),了解基质效应对检测结果的影响,解释在CsA室问质评结果中两方法的检测结果的差别,并找到纠正方法.方法选择不同浓度的临床全血标本100份,用FPIA和EMIT技术进行检测,对比检测结果;通过添加Cu2+,zn2+评估离子残留对免疫反应的影响;用添加等体积血红蛋白富集液后再次抽提的方法,评价非全血基质对质控品和CsA标准品抽提率的影响.结果检测临床全血标本FPIA(X)和EMIT(Y)检测结果具有良好的相关性(Y=0.926 8X-8.115,R2=0.996 9);金属离子残留对FPIA法和EMIT法均不产生下扰(P>0.05);非全血基质样本会降低两方法的抽提率;样本中血红蛋白含量≥30 g/L就可纠正非全血基质对抽提率的影响.结论下发质控品的非全血基质是导致CsA室间质评结果中两系统的检测结果不一致的主要原因,可通过添加血红蛋白富集液进行纠正;遇器官移植受者有极低血红蛋白(<30 g/L)时,要允分考虑低血红蛋白对CsA检测结果的影响.
목적 통과형광편진면역측정(fluorescence polarization immunoassay,FPIA)화매방대면역측정기술(enzyme-multiplied immunoassay technique,EMIT)측정배포소A(cyciosporine,CsA),료해기질효응대검측결과적영향,해석재CsA실문질평결과중량방법적검측결과적차별,병조도규정방법.방법 선택불동농도적림상전혈표본100빈,용FPIA화EMIT기술진행검측,대비검측결과;통과첨가Cu2+,zn2+평고리자잔류대면역반응적영향;용첨가등체적혈홍단백부집액후재차추제적방법,평개비전혈기질대질공품화CsA표준품추제솔적영향.결과 검측림상전혈표본FPIA(X)화EMIT(Y)검측결과구유량호적상관성(Y=0.926 8X-8.115,R2=0.996 9);금속리자잔류대FPIA법화EMIT법균불산생하우(P>0.05);비전혈기질양본회강저량방법적추제솔;양본중혈홍단백함량≥30 g/L취가규정비전혈기질대추제솔적영향.결론 하발질공품적비전혈기질시도치CsA실간질평결과중량계통적검측결과불일치적주요원인,가통과첨가혈홍단백부집액진행규정;우기관이식수자유겁저혈홍단백(<30 g/L)시,요윤분고필저혈홍단백대CsA검측결과적영향.
Objective To explore the matrix effect on cyclosporine A (CsA) test by fluorescence polarization immunoassay (FPIA) and enzyme-multiplied immunoassay technique (EMIT), explain the discrepancy of external quality control results between these two methods and find the corrective action.Methods One hundred whole blood samples with various concentrations were adopted and CsA levels were detected by FPIA and EMIT.The results were compared with each other.Moreover, the influence of residual metal ions upon immunoreactions was assessed by adding Cu2+ and Zn2+.The effect of non-whole blood matrix on extraction efficiency for quality control materials and CsA calibrator was evaluated by adding identical volume of Hb-rich reagents followed with re-extraction.Results There is good correlation between results measured with FPIA(X) and EMIT(Y) methods ( Y=0.926 8X -8.115,R2 =0.996 9).Neither FPIA nor EMIT was affected by residual metal ions ( P ＞ 0.05 ). Non-whole blood matrix decreased the extraction efficiency of two methods, but it could be corrected by supplementation of the Hb-rich reagents (≥30 g/L).Conclusions Non-whole blood matrix may be the main reason for the inconsistent results measured by FPIA and EMIT methods.It could be corrected by using Hb-rich reagents.In addition,we should consider the influence of low lib on CsA test,espocially for organ transplant patients with lower Hb ( ＜30 g/L).