肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2008年
4期
234-236
,共3页
肠肿瘤%Th1细胞%Th2细胞%细胞因子类%流式细胞术
腸腫瘤%Th1細胞%Th2細胞%細胞因子類%流式細胞術
장종류%Th1세포%Th2세포%세포인자류%류식세포술
Intestinalneoplasms%Thl cells%Th2 cells%Cytokines%Flow cytometry
目的 检测大肠癌患者外周血CD+4T细胞分泌细胞因子水平的变化,分析Th1/Th2类细胞因子的平衡调节及其临床意义.方法 刺激细胞,增加细胞内因子表达,荧光标记特异性抗细胞因子单克隆抗体,特异性抗原抗体结合,用流式细胞术分析,检测特异性细胞因子表达水平.结果 大肠癌患者Th1型细胞因子干扰素-γ(IFN-γ)、白细胞介素-2(IL-2)表达水平较健康组显著降低;Th2型细胞因子白细胞介素-4(IL-4)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)表达水平较健康组升高,且IFN-γ/IL-4的比值最具临床意义,差异有统计学意义.结论 大肠癌患者体内Th1/Th2出现漂移,IFN-γ/IL-4比值可作为大肠癌临床进展及免疫功能评价的指标.
目的 檢測大腸癌患者外週血CD+4T細胞分泌細胞因子水平的變化,分析Th1/Th2類細胞因子的平衡調節及其臨床意義.方法 刺激細胞,增加細胞內因子錶達,熒光標記特異性抗細胞因子單剋隆抗體,特異性抗原抗體結閤,用流式細胞術分析,檢測特異性細胞因子錶達水平.結果 大腸癌患者Th1型細胞因子榦擾素-γ(IFN-γ)、白細胞介素-2(IL-2)錶達水平較健康組顯著降低;Th2型細胞因子白細胞介素-4(IL-4)、白細胞介素-6(IL-6)、白細胞介素-10(IL-10)錶達水平較健康組升高,且IFN-γ/IL-4的比值最具臨床意義,差異有統計學意義.結論 大腸癌患者體內Th1/Th2齣現漂移,IFN-γ/IL-4比值可作為大腸癌臨床進展及免疫功能評價的指標.
목적 검측대장암환자외주혈CD+4T세포분비세포인자수평적변화,분석Th1/Th2류세포인자적평형조절급기림상의의.방법 자격세포,증가세포내인자표체,형광표기특이성항세포인자단극륭항체,특이성항원항체결합,용류식세포술분석,검측특이성세포인자표체수평.결과 대장암환자Th1형세포인자간우소-γ(IFN-γ)、백세포개소-2(IL-2)표체수평교건강조현저강저;Th2형세포인자백세포개소-4(IL-4)、백세포개소-6(IL-6)、백세포개소-10(IL-10)표체수평교건강조승고,차IFN-γ/IL-4적비치최구림상의의,차이유통계학의의.결론 대장암환자체내Th1/Th2출현표이,IFN-γ/IL-4비치가작위대장암림상진전급면역공능평개적지표.
Objective To detect the levels of Th1 and Th2 type cytokines of peripheral CD+4 T lymphocytes in patients with colonic cancer in order to find out the significance of variance of imbalance in clinical therapy.Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance the expression of cytokines.Then a specific fluorochrome-conjugated monoclonal antibody was added to be combined with its specific antigen.Cytokines were detected by flow cytometry.Results The levels of Th1 type cytokines such as interferon-γ/(INF-γ),interleukin-2(IL-2)were significantly lower in patients of colonic cancer than those of normal controls.The levels of Th2 type cytokines such as interleukin-4(IL-4),interleukin-6(IL-6)and interleukin-10(IL-10)were higher in patients of colonic cancer than those of normal controls.Conclusion Th1/Th2 shihs in colonic cancer.It is suggested that the levels of INF-γ/IL-4 in colonic cancer may provide clinical index for the prognosis and evaluation of immunity.
