CAJ | 학술논문

目的探讨DNA修复基因X线修复交叉互补因子3(X-ray cross-complementing group 3,XRCC3)基因Thr241Met多态性与黄曲霉毒素B1(aflatoxin B1,AFB1)相关性肝细胞癌(hepatocellular carcinoma,HCC)遗传易感性的相关性.方法应用PCR技术对AFB1高污染区广西地区257例HCC患者和711名对照人群的XRCC3基因多态性进行检测,进行的病例对照研究.结果 (1)XRCC3 3种基因型(Thr/Thr、Thr/Met、Met/Met)中带有Met者与HCC的易感性相关,且这种相关性与Met数量呈正相关(校正风险值OR分别为2.20和8.56);(2)XRCC3突变基因型多态与血白细胞AFB1-DNA加合物水平在HCC发生过程中存在协同作用(校正OR:2.34～20.44,P＜0.01).结论 XRCC3多态性与HCC易感性相关,且这种多态性与AFB1暴露水平在HCC发生中存在协同作用.
목적 탐토DNA수복기인X선수복교차호보인자3(X-ray cross-complementing group 3,XRCC3)기인Thr241Met다태성여황곡매독소B1(aflatoxin B1,AFB1)상관성간세포암(hepatocellular carcinoma,HCC)유전역감성적상관성.방법 응용PCR기술대AFB1고오염구엄서지구257례HCC환자화711명대조인군적XRCC3기인다태성진행검측,진행적병례대조연구.결과 (1)XRCC3 3충기인형(Thr/Thr、Thr/Met、Met/Met)중대유Met자여HCC적역감성상관,차저충상관성여Met수량정정상관(교정풍험치OR분별위2.20화8.56);(2)XRCC3돌변기인형다태여혈백세포AFB1-DNA가합물수평재HCC발생과정중존재협동작용(교정OR:2.34～20.44,P＜0.01).결론 XRCC3다태성여HCC역감성상관,차저충다태성여AFB1폭로수평재HCC발생중존재협동작용.
Objective To explore the association of the Thr241Met polymorphism of X-my cross-complementing group 3(xRcc3) gene with genetic susceptibility to aflatoxin B1(AFBl)-related hepatocellular carcinoma(HCC)in Guangxi population.Methods We conducted a hospital-based case-control study,including 257 HCC cascs and 711 controls without cancels or liver diseases.The XRCC3 Thr241Met polymorphism was analyzed by PCR.Results The XRCC3 genotypes XRCC3.Thr/Met or XRCC3-Met/Met were related with an elevated risk of HCC.The risk of HCC was associated with the number of mutant Met copies(adjusted OR were 2.20 and 8.56 for XRCC3.Thr/Met and Met/Met,respectively);moreover,there seemed tO be combined effects for HCC risk between the variant genotypes and AFBl DNA adduct levels from peripheral blood leukocytes(adjusted OR was 2.34 to 20.44,P<0.01).Conclusion These results suggested that XRCC3 polymorphism may be associated with the risk of AFBl-related HCC among the Guansxipopulation,and interacts with AFBl exposure in the development of HCC induced by AFBl.