西南师范大学学报(自然科学版)
西南師範大學學報(自然科學版)
서남사범대학학보(자연과학판)
JOURNAL OF SOUTHWEST CHINA NORMAL UNIVERSITY
2007年
3期
126-131
,共6页
徐榕敏%易燚波%阙瑞琦%岑亮%孙焕顷%唐云明
徐榕敏%易燚波%闕瑞琦%岑亮%孫煥頃%唐雲明
서용민%역일파%궐서기%잠량%손환경%당운명
中华芦荟(Aloe vera var.chinensis(Haw)Berg.)%超氧化物歧化酶%纯化%性质
中華蘆薈(Aloe vera var.chinensis(Haw)Berg.)%超氧化物歧化酶%純化%性質
중화호회(Aloe vera var.chinensis(Haw)Berg.)%초양화물기화매%순화%성질
Chinese aloe (Aloe vera var. chinensis (Haw) Berg. )%superoxide dismutase (SOD)%purification%properties
芦荟粗提取液经丙酮沉淀、热处理、DEAE-琼脂糖柱层析和Sephacryl S-200凝胶过滤纯化后得到3种电泳纯的SOD组分,即SOD-Ⅰ、SOD-Ⅱ、SOD-Ⅲ.分子量分别为46 000 D,35 000 D,40 000 D;亚基分子量分别为23 000 D,17 000 D,20 000 D,说明芦荟SOD三种组分均为二具体,其等电点分别为8.90,7.60,7.35.抑制剂敏感性实验和元素分析结果表明3种芦荟SOD组分均为Fe-SOD.
蘆薈粗提取液經丙酮沉澱、熱處理、DEAE-瓊脂糖柱層析和Sephacryl S-200凝膠過濾純化後得到3種電泳純的SOD組分,即SOD-Ⅰ、SOD-Ⅱ、SOD-Ⅲ.分子量分彆為46 000 D,35 000 D,40 000 D;亞基分子量分彆為23 000 D,17 000 D,20 000 D,說明蘆薈SOD三種組分均為二具體,其等電點分彆為8.90,7.60,7.35.抑製劑敏感性實驗和元素分析結果錶明3種蘆薈SOD組分均為Fe-SOD.
호회조제취액경병동침정、열처리、DEAE-경지당주층석화Sephacryl S-200응효과려순화후득도3충전영순적SOD조분,즉SOD-Ⅰ、SOD-Ⅱ、SOD-Ⅲ.분자량분별위46 000 D,35 000 D,40 000 D;아기분자량분별위23 000 D,17 000 D,20 000 D,설명호회SOD삼충조분균위이구체,기등전점분별위8.90,7.60,7.35.억제제민감성실험화원소분석결과표명3충호회SOD조분균위Fe-SOD.
Superoxide dismutase (SOD) from Aloe vera var. chinensis (Haw) Berg was purified to electrophoretic homogeneity by a sequential steps , including of acetone and heat of treatment, DEAE-sepharose and Sephacryl S-200 column chromatography. Three SOD components were obtained, designated as SOD-Ⅰ , SOD-Ⅱ, SOD-Ⅲ with the molecular weight of 46 000 D, 35 000 D and 40 000 D, and isoelectric point of 8. 9, 7. 6, 7. 35 respectively. The three components were consisted of two identical subunits. All three SOD components from A. saponaria (Ait) Haw were Fe-SOD as confirmed by its sensitivity to KCN and H2O2, and elemental analyses.
