CAJ | 학술논문

以中华大蟾蜍(Bufo gargarizans gargarizans)蝌蚪为研究对象,采用微核实验和碱性单细胞凝胶电泳法(又称彗星实验,SCGE)检测不同质量浓度(0.1,0.2,0.3,0.4 mg/L)的丁草胺溶液对中华大蟾蜍蝌蚪红细胞的遗传毒性.结果表明:在实验室条件下,丁草胺质量浓度越高,蝌蚪红细胞的微核率和核异常率越显著.经丁草胺溶液处理24 h,蝌蚪红细胞的细胞损伤率及DNA损伤程度(彗星DNA长宽比)出现极显著的提高,并与丁草胺质量浓度呈显著的线性关系.研究表明丁草胺对两栖动物具有遗传毒性作用,同时也说明微核实验和碱性单细胞凝胶电泳法是检测环境污染对两栖动物遗传毒性的合适方法.
이중화대섬서(Bufo gargarizans gargarizans)과두위연구대상,채용미핵실험화감성단세포응효전영법(우칭혜성실험,SCGE)검측불동질량농도(0.1,0.2,0.3,0.4 mg/L)적정초알용액대중화대섬서과두홍세포적유전독성.결과표명:재실험실조건하,정초알질량농도월고,과두홍세포적미핵솔화핵이상솔월현저.경정초알용액처리24 h,과두홍세포적세포손상솔급DNA손상정도(혜성DNA장관비)출현겁현저적제고,병여정초알질량농도정현저적선성관계.연구표명정초알대량서동물구유유전독성작용,동시야설명미핵실험화감성단세포응효전영법시검측배경오염대량서동물유전독성적합괄방법.
Micro-nuclear test and the alkaline single-cell gel electrophoresis assay (SCGE) or comet assay was performed to examine the genotoxicity of butachlor on erythro-cytes of Bufo gargarizans gargarizans tadpoles exposed to increasing concentration of the tested substance (0.1,0.2,0.3,0.4 mg/L). The results showed that both micro-nuclear frequency and nuclear abnormity frequency were significantly increased with the increasing concentration of butachlor in laboratory condition. Tadpoles treated for 24 h had significant, dose-responsive increases in the levels of DNA damage, as measured by the mean DNA length: width ratio. There were strong linear correlations between cells damage frequency, the mean DNA damage (length-to-width ratios) and the concentrations of butachlor. The results of this study indicate that butachlor produce genotoxicity in amphibians, and that using micro-nuclear test and SCGE may be useful for measuring DNA damage in tadpoles exposed in the field.