CAJ | 학술논문

目的: 探讨β-胡萝卜素(β-C)对大鼠急性放射性损伤小肠黏膜结构和屏障功能保护作用的影响. 方法: 将40只大鼠随机分为四组,即对照组(C组)、单纯照射组(R组)、β-C 5 mg/(kg·d)组(T1组)和β-C 10 mg/(kg·d)组(T2组).连续灌胃14 d后,R组、T1组、T2组用直线加速器,以9 Gy剂量进行全腹一次性照射,第4天处死大鼠,测定血浆二胺氧化酶(DAO)活性和内毒素浓度,留取空肠组织,观察肠黏膜形态,测定绒毛隐窝轴(CVA)长度. 结果: R组大鼠体质量增长缓慢,CVA明显短于C组(P<0.01),肠黏膜损伤严重(P<0.008 3);血浆内毒素浓度较C组显著升高(P<0.01),两组间DAO活性无显著性差异.T1组大鼠CVA明显长于R组(P<0.01),肠黏膜损伤轻于R组(P<0.008 3);血浆内毒素浓度较R组明显降低(P<0.01);两组间DAO活性无显著性差异(P>0.05).T2组大鼠体质量较T1组显著增长(P<0.01),CVA明显长于T1组(P<0.01),肠黏膜损伤轻于T1组(P<0.008 3);血浆内毒素浓度较T1组明显降低(P<0.01);DAO活性无显著性差异(P>0.05). 结论: 补充10 mg/(kg·d)β-C可减轻X线照射引起的大鼠小肠黏膜损伤,对维持黏膜屏障功能有一定的保护作用.
목적: 탐토β-호라복소(β-C)대대서급성방사성손상소장점막결구화병장공능보호작용적영향. 방법: 장40지대서수궤분위사조,즉대조조(C조)、단순조사조(R조)、β-C 5 mg/(kg·d)조(T1조)화β-C 10 mg/(kg·d)조(T2조).련속관위14 d후,R조、T1조、T2조용직선가속기,이9 Gy제량진행전복일차성조사,제4천처사대서,측정혈장이알양화매(DAO)활성화내독소농도,류취공장조직,관찰장점막형태,측정융모은와축(CVA)장도. 결과: R조대서체질량증장완만,CVA명현단우C조(P<0.01),장점막손상엄중(P<0.008 3);혈장내독소농도교C조현저승고(P<0.01),량조간DAO활성무현저성차이.T1조대서CVA명현장우R조(P<0.01),장점막손상경우R조(P<0.008 3);혈장내독소농도교R조명현강저(P<0.01);량조간DAO활성무현저성차이(P>0.05).T2조대서체질량교T1조현저증장(P<0.01),CVA명현장우T1조(P<0.01),장점막손상경우T1조(P<0.008 3);혈장내독소농도교T1조명현강저(P<0.01);DAO활성무현저성차이(P>0.05). 결론: 보충10 mg/(kg·d)β-C가감경X선조사인기적대서소장점막손상,대유지점막병장공능유일정적보호작용.
Objective: To investigate effects of beta-carotene on instestine mucosa barrier function in rats damaged by X-ray radiation. Methods: 40 female SD rats were randomized into 4 groups as the normal control group (Group C) ,the radiation group (Group R) ,the β-C 5 mg/(kg · d) group (Group T1) and the β-C 10 mg/(kg · d) group (Group T2). After 14 days of continuous administration of peanut oil in groups C and R or beta-carotene (2. 5 mL/kg) in groups T1 and T2,the rats in groups R, T1 and T2 were radiated under a 9 Gy dose of X-ray. And then 3 days later,the rats were killed and the amount of diamine oxidase(DAO) and the level of bacterial endotoxin were detected. The structure and length of the crypt-villus axis (CVA) of jejunum were also observed and analyzed. Results: Obvious slow weight gain was observed in group R. Compared with group C, the CVA of group R was significantly shorter (P<0.01), and the intestinal mucosal injury was more serious(P <0. 008 3). The plasma endotoxin concentration of group R was significantly higher than that of group C(P<0. 01). There was no significant difference in the activities of DAO between groups R and C. There was no difference in weight gain between groups T1 and R(P>0.05). The CVA of group T1 was significantly longer than that of group R, but the intestinal mucosal injury was slighter(P <0.008 3). The plasma endotoxin concentration of group T1 was significantly lower than that of group R(P <0. 01). There was no significant difference in the activities of DAO between groups T1 and R (P > 0. 05). Group T2 gained more than group T1 (P < 0. 01) . The CVA of group T2 was significantly longer than that of group T1 (P <0. 01) , and the intestinal mucosal injury was slighter(P <0. 008 3). The plasma endotoxin concentration of group T2 was significantly lower than that of group T1 (P <0. 01). There was no significant difference in the activities of DAO between groups T2 and T1 (P >0. 05). Conclusion: Beta-carotene may decrease the X-ray radiation damage on jejunum and maintain the normal function of gut mucosa barrier in rats.