CAJ | 학술논문

本文建立了采用微波辅助间歇提取三七样品中的皂苷类化合物,并以反相高效液相色谱(RP-HPLC)测定其中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的实验方法.分别通过单因素实验和正交实验设计,优化了萃取溶剂浓度、溶剂用量、微波功率和微波辐射时间等提取条件.与传统的索氏提取法、冷浸法和超声波提取法比较,微波辅助萃取法具有快速、提取率高、溶剂消耗少等优点,间歇萃取较连续萃取更是大大节省了微波能耗.
본문건립료채용미파보조간헐제취삼칠양품중적조감류화합물,병이반상고효액상색보(RP-HPLC)측정기중삼칠조감R1、인삼조감Rg1화인삼조감Rb1함량적실험방법.분별통과단인소실험화정교실험설계,우화료췌취용제농도、용제용량、미파공솔화미파복사시간등제취조건.여전통적색씨제취법、랭침법화초성파제취법비교,미파보조췌취법구유쾌속、제취솔고、용제소모소등우점,간헐췌취교련속췌취경시대대절성료미파능모.
A detail procedure for intermittently extracting Notoginseng Saponin R1, Ginseng Saponin Rg1 and Rb1 from Radix Notoginseng was developed by using an obturated microwave-assisted extraction(MAE) device with pressure and temperature controlling units. The effects of solvent concentration, ratio of solution to solid, microwave power and radia-tion time were evaluated by single factor and orthogonal experiments. Compared with Soxhlet extraction, cold-maceration and ultrasonic extraction, microwave extraction was a rapid method with a higher yield and less solvent consumption. Less microwave energy was consumed by using intermittent microwave extraction than continuous microwave extraction.