CAJ | 학술논문

目的研究醛固酮对大鼠主动脉bax基因表达的影响.方法 32只SD大鼠随机分为空白对照组、腺瘤组、腺瘤+依普利酮组和腺瘤+肼苯哒嗪组.在每只大鼠皮下埋植的微量渗透泵内注入空白溶剂或醛固酮.8周后通过免疫组化、RT-PCR和Western 印迹检测主动脉bax基因的表达.结果与对照组相比,腺瘤组大鼠主动脉bax mRNA和蛋白表达都显著上调(P＜0.05);依普利酮能够抑制醛固酮对bax基因的诱导作用(P＜0.05);而肼苯哒嗪虽然可以使大鼠收缩压下降,但不能阻止醛固酮对bax基因的作用.结论醛固酮通过诱导bax基因表达,调节血管平滑肌细胞凋亡和干预细胞周期进程,可能是其导致血管重构的机制之一.
목적 연구철고동대대서주동맥bax기인표체적영향.방법 32지SD대서수궤분위공백대조조、선류조、선류+의보리동조화선류+정분달진조.재매지대서피하매식적미량삼투빙내주입공백용제혹철고동.8주후통과면역조화、RT-PCR화Western 인적검측주동맥bax기인적표체.결과 여대조조상비,선류조대서주동맥bax mRNA화단백표체도현저상조(P＜0.05);의보리동능구억제철고동대bax기인적유도작용(P＜0.05);이정분달진수연가이사대서수축압하강,단불능조지철고동대bax기인적작용.결론 철고동통과유도bax기인표체,조절혈관평활기세포조망화간예세포주기진정,가능시기도치혈관중구적궤제지일.
Objective To study the effect of aldosterone on expression of bax gene in rat aorta.Methods 32 Sprague-Dawley rats bearing an osmotic mini-pump that infuses vehicle or aldosterone(1 μg/h),were randomly divided into 4 groups:control;adenoma;adenoma plus eplerenone(100 mg/kg/day)and adenoma plus hydralazine(25 mg/kg/day).After 8 weeks,the expression of bax gene in the aorta was examined at both mRNA and protein levels by immunohistochemistry,RT-PCR and Western blot.Results Compared with controls,the expression of bax mRNA and protein in the aorta was significantly upregulated in adenoma group(P＜0.05).This upregulation was inhibited by eplerenone but not by hydralazine.Conclusion The upregulated bax gene by aldosterone via mineralocorticoid receptor independently of blood pressure,may be involved in aldosterone-induced vascular remodeling.