CAJ | 학술논문

猴子的大脑皮层主要由灰质和神经元组成,是大脑中调控行动和思维的区域.选取在生命成长过程中具有代表性的3个年龄段(幼年、成年、老年)猴子的大脑皮层组织,首次测得其拉曼光谱.实验结果显示,3个不同年龄段的猴子大脑皮层的拉曼光谱最明显的差异表现在1 000～1 400 cm~(-1)和2 800～3 000cm~(-1)范围内.随着年龄的增长,归属于脂类的拉曼峰1 313和2 885 cm~(-1)的强度越来越强,而归属于蛋白的拉曼峰1 338和2 932 cm~(-1)的强度却逐渐减弱,并且在老年猴子大脑皮层的拉曼谱中出现了2个归属于脂类的拉曼峰1 296和2 850 cm~(-1),I_(1313)/I_(1338)和I_(2885)/I_(2932)的变化趋势很好地描述了这种差异.研究表明,随着年龄的不断增长,猴子大脑皮层中脂类的含量逐渐增加,这样可以很好地说明大脑皮层中脂类的聚积是导致衰老的一个重要原因,为进一步研究人类和动物的生命活动规律、疾病的形成机理提供了很有价值的参考.
후자적대뇌피층주요유회질화신경원조성,시대뇌중조공행동화사유적구역.선취재생명성장과정중구유대표성적3개년령단(유년、성년、노년)후자적대뇌피층조직,수차측득기랍만광보.실험결과현시,3개불동년령단적후자대뇌피층적랍만광보최명현적차이표현재1 000～1 400 cm~(-1)화2 800～3 000cm~(-1)범위내.수착년령적증장,귀속우지류적랍만봉1 313화2 885 cm~(-1)적강도월래월강,이귀속우단백적랍만봉1 338화2 932 cm~(-1)적강도각축점감약,병차재노년후자대뇌피층적랍만보중출현료2개귀속우지류적랍만봉1 296화2 850 cm~(-1),I_(1313)/I_(1338)화I_(2885)/I_(2932)적변화추세흔호지묘술료저충차이.연구표명,수착년령적불단증장,후자대뇌피층중지류적함량축점증가,저양가이흔호지설명대뇌피층중지류적취적시도치쇠로적일개중요원인,위진일보연구인류화동물적생명활동규률、질병적형성궤리제공료흔유개치적삼고.
Monkey cerebral cortex, an important part in the brain to control action and thought activities, is mainly composed of grey matter and nerve cell In the present paper, the in situ Raman spectra of the cerebral cortex of the birth, teenage and aged monkeys were achieved for the first time. The results show that the Raman spectra for the different age monkey cerebral cortex exhibit most obvious changes in the regions of 1 000-1 400 and 2 800-3 000 cm~(-1). With monkey growing up, the relative intensities of the Raman bands at 1 313 and 2 885 cm~(-1) mainly assigned to CH_2 chain vibrational mode of lipid become stronger and stronger whereas the relative intensities of the Raman bands at 1 338 and 2 932 cm~(-1) mainly assigned to CH_3 chain vibrational mode of protein become weaker and weaker. In addition, the two new Raman bands at 1 296 and 2 850 cm~(-1) are only observed in the aged monkey cerebral cortex, therefore, the two bands can be considered as a character or "marker" to differentiate the caducity degree with monkey growth. In order to further explore the changes, the relative intensity ratios of the Raman band at 1 313 cm~(-1) to that at 1 338 cm~(-1) and the Raman band at 2 885 cm~(-1) to that at 2 932 cm~(-1), I_(1313)/I_(1338) and I_(2885)/I_(2932), which are the lipid-to-protein ratios, are introduced to denote the degree of the lipid content The results show that the relative intensity ratios increase significantly with monkey growth, namely, the lipid content in the cerebral cortex increases greatly with monkey growth. So, the authors can deduce that the overmuch lipid is an important cause to induce the caducity. Therefore, the results will be a powerful assistance and valuable parameter to study the order of life growth and diagnose diseases.