CAJ | 학술논문

目的:研究松果体摘除及补充褪黑素对大鼠胸腺巨噬细胞酪氨酸激酶受体B(trkB)表达的影响.方法:选用2月龄清洁级SD大鼠,分为正常对照组、假手术对照组、松果体摘除组、松果体摘除+褪黑素注射7.5mg·kg~(-1)·d~(-1)组和松果体摘除+褪黑素腹腔15mg·kg~(-1)·d~(-1)组,术后4、 8周取材.应用trkB和单克隆抗体ED1免疫荧光双标显色法观察分析松果体摘除及补充外源性褪黑素后胸腺巨噬细胞trkB表达的变化.结果:与对照组相比,松果体摘除术后4周胸腺双阳性细胞表达减弱,补充褪黑素后无变化.术后8周松果体摘除组与对照组相比无明显差异,而低剂量组双阳性细胞表达异常增高,高剂量组恢复正常.各组平均灰度无明显差异.结论:松果体及褪黑素在影响胸腺细胞分化发育过程中,胸腺巨噬细胞表达的trkB也随之发生变化,其与胸腺细胞表达的trkB共同作用参与胸腺细胞分化发育.胸腺神经内分泌微环境的分泌调节是松果体及褪黑素保护胸腺细胞的通路之一.
목적:연구송과체적제급보충퇴흑소대대서흉선거서세포락안산격매수체B(trkB)표체적영향.방법:선용2월령청길급SD대서,분위정상대조조、가수술대조조、송과체적제조、송과체적제+퇴흑소주사7.5mg·kg~(-1)·d~(-1)조화송과체적제+퇴흑소복강15mg·kg~(-1)·d~(-1)조,술후4、 8주취재.응용trkB화단극륭항체ED1면역형광쌍표현색법관찰분석송과체적제급보충외원성퇴흑소후흉선거서세포trkB표체적변화.결과:여대조조상비,송과체적제술후4주흉선쌍양성세포표체감약,보충퇴흑소후무변화.술후8주송과체적제조여대조조상비무명현차이,이저제량조쌍양성세포표체이상증고,고제량조회복정상.각조평균회도무명현차이.결론:송과체급퇴흑소재영향흉선세포분화발육과정중,흉선거서세포표체적trkB야수지발생변화,기여흉선세포표체적trkB공동작용삼여흉선세포분화발육.흉선신경내분비미배경적분비조절시송과체급퇴흑소보호흉선세포적통로지일.
Objective: To study the effect of pinealectomy (Px) and melatonin (MLT) supplementation upon macrophages tryrosin kinase receptor B (trkB) expression in rat thymus. Methods: The clean 2-month-old rats were divided into five groups: normal control group, sham operation group, pinealectomized group, pinealectomy +7.5mg·kg~(-1)·d~(-1) MLT intraperitoneal injection group and pinealectomy +15mg·kg~(-1)·d~(-1) MLT intraperitoneal injection group, and their thymus were taken out at 4th week and 8th after operation. Double-immunofluorescent labeling for trkB and ectodermal dysplasia 1(ED1) antibody was used to observe and analyze the changes of double positive cells in thymus after Px and MLT supplementation. Results: Compared to the normal and sham group, the expression of thymic trkB~+ ED1~+ macrophages was weakened at 4th week after Px. MLT supplementation made no difference in the number of double positive cells and positive areas at the same time. But trkB~+ ED1~+ macrophage showed no significant change compared to the normal and sham group at the 8th week after Px, and the expression of macrophages increased significantly in the low dose group at the 8th week, and returned to normal in the high dose group at the 8th week. The average gray scale of double positive cells showed no significant difference among all groups. Conclusion: Macrophage trkB expression of thymic is also influenced by pineal gland and MLT, which participate in the differentiation and development of thymocytes with trkB expression. The adjustment of thymus neuroendocine microenvironment is one of the gateways in maintaining the thymocytes by pineal gland and MLT.