CAJ | 학술논문

背景:转化生长因子β在肌腱愈合与粘连形成中具有重要的作用,抑制转化生长因子β及其受体表达可起到一定的防止肌腱术后粘连作用.目的:探讨转化生长因子β天然抑制剂6-磷酸果糖对兔屈趾肌腱腱鞘、腱外膜和腱内膜细胞转化生长因子β及其受体的影响.方法:取兔屈趾肌腱分离培养腱鞘、腱外膜和腱内膜细胞.3种细胞分别加入6-磷酸果糖(实验组)或不加6-磷酸果糖(对照组)培养.采用酶联免疫吸附实验定量检测转化生长因子β及其受体的表达,原位杂交和免疫组织化学观察观测转化生长因子β1的表达. 结果与结论:实验组细胞转化生长因子β及其受体的表达均较对照组下降(P < 0.05).实验组3种细胞阳性转化生长因子β1 mRNA表达率及细胞内转化生长因子β1 mRNA表达强度均较对照组明显降低(P < 0.05).免疫组化显示加入6-磷酸果糖培养后,3种细胞转化生长因子β1表达均明显降低.
배경:전화생장인자β재기건유합여점련형성중구유중요적작용,억제전화생장인자β급기수체표체가기도일정적방지기건술후점련작용.목적:탐토전화생장인자β천연억제제6-린산과당대토굴지기건건초、건외막화건내막세포전화생장인자β급기수체적영향.방법:취토굴지기건분리배양건초、건외막화건내막세포.3충세포분별가입6-린산과당(실험조)혹불가6-린산과당(대조조)배양.채용매련면역흡부실험정량검측전화생장인자β급기수체적표체,원위잡교화면역조직화학관찰관측전화생장인자β1적표체. 결과여결론:실험조세포전화생장인자β급기수체적표체균교대조조하강(P < 0.05).실험조3충세포양성전화생장인자β1 mRNA표체솔급세포내전화생장인자β1 mRNA표체강도균교대조조명현강저(P < 0.05).면역조화현시가입6-린산과당배양후,3충세포전화생장인자β1표체균명현강저.
BACKGROUND: Transforming growth factor beta (TGF-β) has an important role in tendon healing and adhesion formation.Inhibiting TGF-β and its receptor expression may prevent adhesions after tendon open.OBJECTIVE: To study the effects of mannose-6-phosphate, a natural inhibitor of TGF-β, on TGF-β and its receptor production in tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes of rabbit flexor toes.METHODS: Tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes were isolated from rabbit flexor tendon and cultured separately. All these cells were divided into 2 groups at random, experiment group supplemented with mannose-6-phosphate and control group without mannose-6-phosphate. The expression of TGF-β and TGF-β receptor was quantified with enzyme-linked immunosorbent assay. The expression of TGF-β1 was also assessed with in situ hybridization and immunohistochemistry.RESULTS AND CONCLUSION: The expression of TGF-β and TGF-β receptor in experiment group was significantly lower than that in control group (P ＜ 0.05). In experimental group, the positive expression of TGF-β1 mRNA and the expression level of intracellular TGF-β1 mRNA in all tendon cells demonstrated significantly lower than those in the control group (P ＜ 0.05).Immunohistochemical staining showed expression of TGF-β1 were significantly lower in all three types of tendon cell cultured with mannose-6-phosphate.