中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2012年
2期
156-160
,共5页
李玲%李振宇%程欢莲%严谨%胡凯%王俊杰%邓晓兰%叶啟发%欧阳冬生
李玲%李振宇%程歡蓮%嚴謹%鬍凱%王俊傑%鄧曉蘭%葉啟髮%歐暘鼕生
리령%리진우%정환련%엄근%호개%왕준걸%산효란%협계발%구양동생
醛糖还原酶%基因多态性%原发性高血压
醛糖還原酶%基因多態性%原髮性高血壓
철당환원매%기인다태성%원발성고혈압
aldose reductase%polymorphism%essential hypertension
目的:研究中国汉族人群中醛糖还原(AR)C-106T基因多态性的分布情况,比较其频率分布是否存在种族差异,并探讨该多态性与原发性高血压易感性的相关性.方法:应用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)的分析方法,在148位原发性高血压病人和137位正常受试者中,对AR基因C-106T多态性进行基因分型.频数分布的比较采用卡方检验,基因型与高血压的关联程度采用95％置信区间评估.结果:对照人群中AR C-106T等位的频率为13.9％ (95％ CI:11.2％～16.6％),远低于日本人群(n=712,18.4％,P=0.0063),澳大利亚人群(n=240,37.9％,P＜0.0001)和巴西人群(n=62,34.7％,P＜0.0001).高血压病例和对照人群中AR-C106T等位基因的频率分别为15.9％(95％ CI:11.6％～20.0％)和11.7％ (95％ CI:7.9％～15.5％),两组间基因型分布差异无统计学意义( P=0.147).结论:醛糖还原酶AR基因C-106T多态性的频率分布具有明显的种族差异,该多态性与中国人群原发性高血压的发病风险不相关.
目的:研究中國漢族人群中醛糖還原(AR)C-106T基因多態性的分佈情況,比較其頻率分佈是否存在種族差異,併探討該多態性與原髮性高血壓易感性的相關性.方法:應用聚閤酶鏈反應-限製性片段長度多態性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)的分析方法,在148位原髮性高血壓病人和137位正常受試者中,對AR基因C-106T多態性進行基因分型.頻數分佈的比較採用卡方檢驗,基因型與高血壓的關聯程度採用95％置信區間評估.結果:對照人群中AR C-106T等位的頻率為13.9％ (95％ CI:11.2％～16.6％),遠低于日本人群(n=712,18.4％,P=0.0063),澳大利亞人群(n=240,37.9％,P＜0.0001)和巴西人群(n=62,34.7％,P＜0.0001).高血壓病例和對照人群中AR-C106T等位基因的頻率分彆為15.9％(95％ CI:11.6％～20.0％)和11.7％ (95％ CI:7.9％～15.5％),兩組間基因型分佈差異無統計學意義( P=0.147).結論:醛糖還原酶AR基因C-106T多態性的頻率分佈具有明顯的種族差異,該多態性與中國人群原髮性高血壓的髮病風險不相關.
목적:연구중국한족인군중철당환원(AR)C-106T기인다태성적분포정황,비교기빈솔분포시부존재충족차이,병탐토해다태성여원발성고혈압역감성적상관성.방법:응용취합매련반응-한제성편단장도다태성(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)적분석방법,재148위원발성고혈압병인화137위정상수시자중,대AR기인C-106T다태성진행기인분형.빈수분포적비교채용잡방검험,기인형여고혈압적관련정도채용95％치신구간평고.결과:대조인군중AR C-106T등위적빈솔위13.9％ (95％ CI:11.2％～16.6％),원저우일본인군(n=712,18.4％,P=0.0063),오대리아인군(n=240,37.9％,P＜0.0001)화파서인군(n=62,34.7％,P＜0.0001).고혈압병례화대조인군중AR-C106T등위기인적빈솔분별위15.9％(95％ CI:11.6％～20.0％)화11.7％ (95％ CI:7.9％～15.5％),량조간기인형분포차이무통계학의의( P=0.147).결론:철당환원매AR기인C-106T다태성적빈솔분포구유명현적충족차이,해다태성여중국인군원발성고혈압적발병풍험불상관.
Objective:To investigate the distribution of aldose reductase (AR) C-106T genetic polymorphism in Chinese Han population and its association with the risk for essential hypertension (EH).Methods:The AR C-106T polymorphism was genotyped in 148 Chinese EH patients and 137controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The genotype distribution between groups was contrasted by x2- test and the degree of genetic association was evaluated by 95％ confidence interval (CI).Results:Frequency of the variant AR C-106T allele was 13.9％ (95％ CI:11.2％-16.6％) in the controls,which was significantly lower than that in the Japanese (18.4％ in 712 individuals,P=0.0063),the Australians (37.9％ in 240 individuals,P＜0.0001) and the Brazilians (34.7％ in 62individuals,P＜ 0.0001).The frequency ofAR C-106T allele was 11.7％ (95％ CI:7.9％-15.5％)in the EH patients.No significant difference in the allele frequency was observed between the EH patients and the controls (P=0.147).Conclusion:There is obvious racial difference in the distribution of AR C-106T polymorphism.The polymorphism is not associated with the risk for EH.
