CAJ | 학술논문

目的探讨支气管哮喘(哮喘)患儿Eotaxin、IL-13及总IgE在哮喘发病中的作用及其相互关系.方法选择30例哮喘儿童及22例健康儿童为研究对象,酶联免疫吸附法检测血清Eotaxin、IL-13,荧光酶联免疫法检测总IgE水平.哮喘息儿同时做肺功能检测.结果 (1)哮喘患儿急性发作期与临床缓解期肺功能各项指标比较差异均有统计学意义(P值均＜0.001);(2)哮喘患儿急性发作期与临床缓解期及与健康对照组比较血清Eotaxin、IL-13、总IgE水平比较差异具有统计学意义(P值均＜0.001).(3)哮喘患儿急性发作期、临床缓解期Eotaxin与IL-13、Eotaxin与总IgE及IL-13与总IgE均呈正相关关系;(4)哮喘患儿血清Eotaxin及IL-13水平与同期肺功能第1秒用力肺活量(FEV1)及呼气高峰流量(PEF)均呈正相关关系(P值均＜0.05),总IgE水平与同期肺功能FEV1及PEF均无明显相关关系(P值均＞0.05).结论血清Eotaxin、IL-13与总IgE可能共同参与哮喘的病理生理改变,血清Eotaxin、IL-13可以作为评判哮喘病情严重程度的客观指标.
목적 탐토지기관효천(효천)환인Eotaxin、IL-13급총IgE재효천발병중적작용급기상호관계.방법 선택30례효천인동급22례건강인동위연구대상,매련면역흡부법검측혈청Eotaxin、IL-13,형광매련면역법검측총IgE수평.효천식인동시주폐공능검측.결과 (1)효천환인급성발작기여림상완해기폐공능각항지표비교차이균유통계학의의(P치균＜0.001);(2)효천환인급성발작기여림상완해기급여건강대조조비교혈청Eotaxin、IL-13、총IgE수평비교차이구유통계학의의(P치균＜0.001).(3)효천환인급성발작기、림상완해기Eotaxin여IL-13、Eotaxin여총IgE급IL-13여총IgE균정정상관관계;(4)효천환인혈청Eotaxin급IL-13수평여동기폐공능제1초용력폐활량(FEV1)급호기고봉류량(PEF)균정정상관관계(P치균＜0.05),총IgE수평여동기폐공능FEV1급PEF균무명현상관관계(P치균＞0.05).결론 혈청Eotaxin、IL-13여총IgE가능공동삼여효천적병리생리개변,혈청Eotaxin、IL-13가이작위평판효천병정엄중정도적객관지표.
Objective To study the nosogenesis and the relationship of different levels of serum Eotaxin,IL-13 and total IgE(TIgE)in asthmatic children. Methods Thirty children with asthma aging from 5 to 12 years were selected as study group (attack period and remission period), and 22 healthy children were selected as control group. Serum Eotaxin and IL-13 were measured with enzyme linked immunosorbent assay, and serum TIgE was determined with enzyme-linked fluoroimmune assay in the study group and control group, and pulmonary function was measured at the same time in the study group. Results (1) There were significant differences in pulmonary function between the attack period and the remission period (P ＜ 0. 001). (2) There were significant differences in the level of serum Eotaxin, IL-13 and IgE between the study group and the control group,especially for the attack period and the control group(P ＜0. 001). (3) There were significant positive correlations between the level of serum Eotaxin and IL-13, Eotaxin and TIgE, IL-13 and TIgE respectively in the study group (P ＜0. 05). (4) There were negative correlcations between the level of serum Eotaxin and FEV1 ,PEF,and serum IL-13 and FEV1, PEF in asthmatic children (P ＜ 0. 05), there were no correlations between the level of serum TIgE and FEV1, PEF in the study group (P ＞ 0. 05). Conclusion Serum Eotaxin, IL-13 and TIgE were participated in the nosogenesis of asthma, and the level of serum Eotaxin and IL-13 can be a assessment of asthmatic patients' condition.