中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2012年
8期
825-829
,共5页
王占青%马玉英%尹彩星%卫肖肖
王佔青%馬玉英%尹綵星%衛肖肖
왕점청%마옥영%윤채성%위초초
思密达%百草枯%血浆浓度%病理变化%治疗作用
思密達%百草枯%血漿濃度%病理變化%治療作用
사밀체%백초고%혈장농도%병리변화%치료작용
Smecta%Paraquat%Plasma concentration%Pathological change%Therapeutic effect
目的 探讨思密达(smecta)对百草枯(paraquat,PQ)中毒大鼠血浆质量浓度的影响及对肺、胃、空肠损伤的防治作用,并分析其作用机制.方法 健康的SD大鼠76只随机(随机数字法)分为健康对照组(A组)6只、PQ (50 mg/kg)灌胃染毒组(B组)30只、思密达( 400 mg/kg)干预组(C组)30只.B组、C组中毒后2、6、24、48、72 h分批处死存活的大鼠,每次6只,测定血浆PQ浓度,取肺、胃、空肠行HE染色及病理学观察并评分.数据通过正态分布和方差齐性检验,浓度比较采用两样本t检验；病理评分采用完全随机设计单因素方差分析,并采用LSD -t检验进行两组之间比较；以P＜0.05为差异具有统计学意义.结果 所有大鼠均无自然死亡,B组PQ血浆浓度(ng/ml)[(4320.6150±413.9471),(440.3144±49.7762)]；肺组织损伤逐渐加重,肺血管扩张充血,灶状出血,肺间隔增厚、大量炎性细胞浸润,局部肺实变；胃组织黏膜明充血,绒毛剥脱,炎症细胞浸润:空肠黏膜出血坏死,局部绒毛脱落,腺体减少,伴大量炎细胞.C组与B组在同一时间点相比,质量浓度明显降低(P＜0.01)肺、胃、空肠病理变化均有不同程度减轻(P＜0.05,P＜0.05或P＜0.01).结论 思密达不仅降低血浆PQ质量浓度,而且减轻百草枯中毒大鼠的病理变化.
目的 探討思密達(smecta)對百草枯(paraquat,PQ)中毒大鼠血漿質量濃度的影響及對肺、胃、空腸損傷的防治作用,併分析其作用機製.方法 健康的SD大鼠76隻隨機(隨機數字法)分為健康對照組(A組)6隻、PQ (50 mg/kg)灌胃染毒組(B組)30隻、思密達( 400 mg/kg)榦預組(C組)30隻.B組、C組中毒後2、6、24、48、72 h分批處死存活的大鼠,每次6隻,測定血漿PQ濃度,取肺、胃、空腸行HE染色及病理學觀察併評分.數據通過正態分佈和方差齊性檢驗,濃度比較採用兩樣本t檢驗；病理評分採用完全隨機設計單因素方差分析,併採用LSD -t檢驗進行兩組之間比較；以P＜0.05為差異具有統計學意義.結果 所有大鼠均無自然死亡,B組PQ血漿濃度(ng/ml)[(4320.6150±413.9471),(440.3144±49.7762)]；肺組織損傷逐漸加重,肺血管擴張充血,竈狀齣血,肺間隔增厚、大量炎性細胞浸潤,跼部肺實變；胃組織黏膜明充血,絨毛剝脫,炎癥細胞浸潤:空腸黏膜齣血壞死,跼部絨毛脫落,腺體減少,伴大量炎細胞.C組與B組在同一時間點相比,質量濃度明顯降低(P＜0.01)肺、胃、空腸病理變化均有不同程度減輕(P＜0.05,P＜0.05或P＜0.01).結論 思密達不僅降低血漿PQ質量濃度,而且減輕百草枯中毒大鼠的病理變化.
목적 탐토사밀체(smecta)대백초고(paraquat,PQ)중독대서혈장질량농도적영향급대폐、위、공장손상적방치작용,병분석기작용궤제.방법 건강적SD대서76지수궤(수궤수자법)분위건강대조조(A조)6지、PQ (50 mg/kg)관위염독조(B조)30지、사밀체( 400 mg/kg)간예조(C조)30지.B조、C조중독후2、6、24、48、72 h분비처사존활적대서,매차6지,측정혈장PQ농도,취폐、위、공장행HE염색급병이학관찰병평분.수거통과정태분포화방차제성검험,농도비교채용량양본t검험；병리평분채용완전수궤설계단인소방차분석,병채용LSD -t검험진행량조지간비교；이P＜0.05위차이구유통계학의의.결과 소유대서균무자연사망,B조PQ혈장농도(ng/ml)[(4320.6150±413.9471),(440.3144±49.7762)]；폐조직손상축점가중,폐혈관확장충혈,조상출혈,폐간격증후、대량염성세포침윤,국부폐실변；위조직점막명충혈,융모박탈,염증세포침윤:공장점막출혈배사,국부융모탈락,선체감소,반대량염세포.C조여B조재동일시간점상비,질량농도명현강저(P＜0.01)폐、위、공장병리변화균유불동정도감경(P＜0.05,P＜0.05혹P＜0.01).결론 사밀체불부강저혈장PQ질량농도,이차감경백초고중독대서적병리변화.
Objective To evaluate therapeutic effect and the possible mechanism of smecta on paraqual plasma concentrations and multiorgans injury induced by paraquat intoxication in rats. Methods A total of 76 healthy adult SD rats were randomly ( random number) divided into group A (control group n =6),group B ( poisoned group n =30 ),group C (smecta-treated group n=30).Rats in groups B and C were treated intragastrically with PQ at 50 mg/kg,the rats in the group C were given with smecta at 50 mg/kg,while the rats in the other two groups were only intragastrically adminstered with saline.Live rats in groups B and C were sacrificed at 2,6,24,48,72 h after administration of PQ for the determination of paraquat plasma concentrations and for HE staining of lung,stomach and jejunum.The rats were executed at the end of trial by the same way in group A.All measurement data were expressed as means + standard deviation ((x) ±s).The data of pathological score were compared with Independent-samples T test and the data of PQ concentration compared with analysis of variance (ANOVA) followed by LSD-t multiple comparison test.P-values of less than 0.05 were considered statistically significant.Results The paraquat plasma concentration ( ng/ml ) was 440.314 ± 49.776 to 4320.6150 ± 413.947.There were different pathological changes of lung,stomach and jejunum in group B. Lung injuries gradually deteriorated,congestion,edema,leukocyte infiltration,incrassated septa and lung consolidation were observed.The pathological changes were obvious such as abruption of mucosa,hyperemic gastric mucosa and leukocyte infiltration in stomach.Haemorrhage of jejunum mucosa,abruption of villus,gland damage and inflammatory cell infiltration were found. Compared with group B,all the pathological changes mentioned above were obviously alleviated in group C ( P ＜ 0.05 ),and the concentrations reduced ( P ＜ 0.01 ).Conclusions Smecta reduced paraquat plasma concentrations and alleviated pathologic injury of rats with PQ poisoning.
