中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2010年
9期
778-781
,共4页
对氧磷脂酶%载脂蛋白E%糖尿病,2型%多态性,基因%氧化型低密度脂蛋白
對氧燐脂酶%載脂蛋白E%糖尿病,2型%多態性,基因%氧化型低密度脂蛋白
대양린지매%재지단백E%당뇨병,2형%다태성,기인%양화형저밀도지단백
Paraoxonase%Apolipoprotein E%Diabetes mellitus,type 2%Polymorphism,gene%Oxidized low-density lipoprotein
采用限制性片段长度多态性(RFLP)检测福建地区192例2型糖尿病组、116例单纯动脉粥样硬化(AS)患者和105名正常人对氧磷脂酶1(PON1)、载脂蛋白E(ApoE)基因多态性,血清氧化型低密度脂蛋白(Ox-LDL)水平采用酶联免疫吸附法检测.结果 显示,2型糖尿组Ox-LDL高于单纯AS组及正常对照组[(754.2±279.9对526.1±186.2和421.1±163.2)μg/L,P<0.01].在2型糖尿患者中,PON1基因QQ型Ox-LDL水平高于QR和RR型[(846.6±147.5对763.4±126.7和713.2±132.4)μg/L,P<0.01];ApoE基因ε3/4+ε4/4型Ox-LDL水平高于ε3/3和ε2/2+ε2/3型[(824.3±173.5对741.6±182.5和718.3±167.5)μg/L,P<0.05],而在单纯AS患者中未发现这种差别.多元同归分析显示,病程、PON1、ApoE是2型糖尿患者血浆Ox-LDL增高的危险因素.
採用限製性片段長度多態性(RFLP)檢測福建地區192例2型糖尿病組、116例單純動脈粥樣硬化(AS)患者和105名正常人對氧燐脂酶1(PON1)、載脂蛋白E(ApoE)基因多態性,血清氧化型低密度脂蛋白(Ox-LDL)水平採用酶聯免疫吸附法檢測.結果 顯示,2型糖尿組Ox-LDL高于單純AS組及正常對照組[(754.2±279.9對526.1±186.2和421.1±163.2)μg/L,P<0.01].在2型糖尿患者中,PON1基因QQ型Ox-LDL水平高于QR和RR型[(846.6±147.5對763.4±126.7和713.2±132.4)μg/L,P<0.01];ApoE基因ε3/4+ε4/4型Ox-LDL水平高于ε3/3和ε2/2+ε2/3型[(824.3±173.5對741.6±182.5和718.3±167.5)μg/L,P<0.05],而在單純AS患者中未髮現這種差彆.多元同歸分析顯示,病程、PON1、ApoE是2型糖尿患者血漿Ox-LDL增高的危險因素.
채용한제성편단장도다태성(RFLP)검측복건지구192례2형당뇨병조、116례단순동맥죽양경화(AS)환자화105명정상인대양린지매1(PON1)、재지단백E(ApoE)기인다태성,혈청양화형저밀도지단백(Ox-LDL)수평채용매련면역흡부법검측.결과 현시,2형당뇨조Ox-LDL고우단순AS조급정상대조조[(754.2±279.9대526.1±186.2화421.1±163.2)μg/L,P<0.01].재2형당뇨환자중,PON1기인QQ형Ox-LDL수평고우QR화RR형[(846.6±147.5대763.4±126.7화713.2±132.4)μg/L,P<0.01];ApoE기인ε3/4+ε4/4형Ox-LDL수평고우ε3/3화ε2/2+ε2/3형[(824.3±173.5대741.6±182.5화718.3±167.5)μg/L,P<0.05],이재단순AS환자중미발현저충차별.다원동귀분석현시,병정、PON1、ApoE시2형당뇨환자혈장Ox-LDL증고적위험인소.
The polymorphisms of paraoxonase 1 ( PON 1 ) and ApoE gene in 192 patients with type 2diabetes mellitus (DM group) , 116 cases of atherosclerosis (AS) without DM (AS group), and 105 normal subjects (NC group ) were detected by means of PCR-restriction fragment length polymorphism. The serum concentration of Ox-LDL was determined by enzyme linked immunosorbent assay. The results showed that plasma Ox-LDL level in DM group was higher than those in AS and NC groups[(754.2±279.9 vs 526. 1±186.2 and 421.1 ± 163.2 )μg/L, P<0.01]. Ox-LDL level in the patients with QQ genotype of PON 1 was higher than those in QR and RR genotypes in type 2 DM[(846.6±147.5 vs 763.4±126. 7 and 7 1 3.2 ±132.4 ) μg/L, P<0. 0l],and Ox-LDL level in the patients with ε3/4+ ε4/4 genotype of ApoE was also higher than those in ε3/3 and ε2/2 + ε2/3 geneotypes in type 2 DM[( 824.3 ± 173.5 vs 741.6± 182. 5 and 718.3 ± 167.5 ) μg/L, P<0.05], but the difference was not found in atherosclerosis without DM. Multiple regression analysis showed that disease duration, PON1 and ApoE genotypes were the independent predictors of plasma Ox-LDL level in patients with type 2 DM.
