中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2010年
6期
548-551
,共4页
宋丹%刘庆淮%王桂云%付金玲%车松天%苏冠方%隋桂琴
宋丹%劉慶淮%王桂雲%付金玲%車鬆天%囌冠方%隋桂琴
송단%류경회%왕계운%부금령%차송천%소관방%수계금
糖尿病视网膜病变/并发症%青光眼,新生血管性/病理学%趋化因子CXCL12%血管内皮生长因子类
糖尿病視網膜病變/併髮癥%青光眼,新生血管性/病理學%趨化因子CXCL12%血管內皮生長因子類
당뇨병시망막병변/병발증%청광안,신생혈관성/병이학%추화인자CXCL12%혈관내피생장인자류
Diabetic retinopathy/complication%Glaucoma,neovascular/pathology%Chemokine CXCL12%Vascular endothelial growth factors
目的 观察基质细胞衍生因子-1α(SDF-1α)在增生性糖尿病视网膜病变(PDR)继发新生血管性青光眼(NVG)中的作用,并探讨其作用机制.方法 采集PDR患者25例31只眼的玻璃体标本.其中,继发NVG者13只眼作为实验组,无NVG者18只眼作为对照组.配制含10、100、1000 ng/ml SDF-1α和10 ng/m1血管内皮生长因子(VEGF)培养液,并以此分组;测量各浓度组与体外对照组人脐静脉内皮细胞(HUVEC)管腔样结构及毛细血管样结构全长.配制含10、100、1000 ng/ml SDF-1α和100 ng/ml血管内皮生长因子(VEGF)培养液,并以此分组;采用5'-溴2'-脱氧尿嘧啶(BrdU)掺入法行细胞增生检测,分析各浓度组与体外对照组吸光度[A,旧称光密度(OD)]值.采用酶联免疫吸附试验(ELISA)检测玻璃体标本实验组和玻璃体标本对照组患者玻璃体标本中VEGF和SDF-1α含量.结果 体外血管生成检测显示,10、100、1000 ng/ml SDF-1α和10 ng/ml VEGF组HUVEC管腔样和毛细血管样结构长度均较体外对照组长,差异均有统计学意义(P<0.05).细胞增生检测显示,10、100、1000 ng/ml SDF-1α和100 ng/ml VEGF 组A值均较体外对照组增高,差异有统计学意义(P<0.05).ELISA法检测显示,玻璃体标本实验组患者玻璃体标本中SDF-1α和VEGF含量均明显高于玻璃体标本对照组,差异有统计学意义(P<0.01).结论 SDF-1α参与了PDR继发NVG的形成过程,可能与SDF-1α促进血管内皮细胞增生而促进新生血管形成有关.
目的 觀察基質細胞衍生因子-1α(SDF-1α)在增生性糖尿病視網膜病變(PDR)繼髮新生血管性青光眼(NVG)中的作用,併探討其作用機製.方法 採集PDR患者25例31隻眼的玻璃體標本.其中,繼髮NVG者13隻眼作為實驗組,無NVG者18隻眼作為對照組.配製含10、100、1000 ng/ml SDF-1α和10 ng/m1血管內皮生長因子(VEGF)培養液,併以此分組;測量各濃度組與體外對照組人臍靜脈內皮細胞(HUVEC)管腔樣結構及毛細血管樣結構全長.配製含10、100、1000 ng/ml SDF-1α和100 ng/ml血管內皮生長因子(VEGF)培養液,併以此分組;採用5'-溴2'-脫氧尿嘧啶(BrdU)摻入法行細胞增生檢測,分析各濃度組與體外對照組吸光度[A,舊稱光密度(OD)]值.採用酶聯免疫吸附試驗(ELISA)檢測玻璃體標本實驗組和玻璃體標本對照組患者玻璃體標本中VEGF和SDF-1α含量.結果 體外血管生成檢測顯示,10、100、1000 ng/ml SDF-1α和10 ng/ml VEGF組HUVEC管腔樣和毛細血管樣結構長度均較體外對照組長,差異均有統計學意義(P<0.05).細胞增生檢測顯示,10、100、1000 ng/ml SDF-1α和100 ng/ml VEGF 組A值均較體外對照組增高,差異有統計學意義(P<0.05).ELISA法檢測顯示,玻璃體標本實驗組患者玻璃體標本中SDF-1α和VEGF含量均明顯高于玻璃體標本對照組,差異有統計學意義(P<0.01).結論 SDF-1α參與瞭PDR繼髮NVG的形成過程,可能與SDF-1α促進血管內皮細胞增生而促進新生血管形成有關.
목적 관찰기질세포연생인자-1α(SDF-1α)재증생성당뇨병시망막병변(PDR)계발신생혈관성청광안(NVG)중적작용,병탐토기작용궤제.방법 채집PDR환자25례31지안적파리체표본.기중,계발NVG자13지안작위실험조,무NVG자18지안작위대조조.배제함10、100、1000 ng/ml SDF-1α화10 ng/m1혈관내피생장인자(VEGF)배양액,병이차분조;측량각농도조여체외대조조인제정맥내피세포(HUVEC)관강양결구급모세혈관양결구전장.배제함10、100、1000 ng/ml SDF-1α화100 ng/ml혈관내피생장인자(VEGF)배양액,병이차분조;채용5'-추2'-탈양뇨밀정(BrdU)참입법행세포증생검측,분석각농도조여체외대조조흡광도[A,구칭광밀도(OD)]치.채용매련면역흡부시험(ELISA)검측파리체표본실험조화파리체표본대조조환자파리체표본중VEGF화SDF-1α함량.결과 체외혈관생성검측현시,10、100、1000 ng/ml SDF-1α화10 ng/ml VEGF조HUVEC관강양화모세혈관양결구장도균교체외대조조장,차이균유통계학의의(P<0.05).세포증생검측현시,10、100、1000 ng/ml SDF-1α화100 ng/ml VEGF 조A치균교체외대조조증고,차이유통계학의의(P<0.05).ELISA법검측현시,파리체표본실험조환자파리체표본중SDF-1α화VEGF함량균명현고우파리체표본대조조,차이유통계학의의(P<0.01).결론 SDF-1α삼여료PDR계발NVG적형성과정,가능여SDF-1α촉진혈관내피세포증생이촉진신생혈관형성유관.
Objective To observe the effects of stromal cell-derived factor 1α (SDF-1α) in secondary neovascular glaucoma (NVG) of proliferative diabetic retinopathy (PDR). Methods The vitreous specimens from 25 PDR patients (31 eyes) were collected with 13 NVG eyes and non-NVG 18 eyes. The concentrations of SDF-1α and vascular endothelial growth factor (VEGF) in those specimens were measured by enzyme-linked immunosorbent assay (ELISA). Human umbilical vein endothelial cells (HUVEC) were treated by different concentrations of SDF-1αand vascular endothelial growth factor (VEGF) in vitro, and the formation of tube cavity-like structure, length of capillary-like structures and 5'-bromo-2'-deoxyuridine (BrdU) labeling of treated HUVEC were measured. Results The length of HUVEC tube-like and capillarylike structure formation in 10, 100, 1000 ng/ml SDF-1α and 10 ng/ml VEGF groups were longer than that in the control group, the differences were statistically significant (P<0. 01). The A value of BrdU labeling of 10, 100, 1000 ng/ml SDF-1α and 10 ng/ml VEGF groups were increased than that in the control group,the differences were statistically significant (P<0.01). The vitreous levels of SDF-1α and VEGF of NVG specimens were higher than those in the non-NVG group, the differences were statistically significant (P<0.01). Conclusions SDF-1α may promote the migration and proliferation of vascular endothelium cells, and participate in the neovascularization process in NVG patients with PDR.