CAJ | 학술논문

目的探讨CD2相关蛋白(CD2AP)及肾质(nephrin)在成人多房囊性肾细胞癌中的表达及意义.方法收集多房囊性肾细胞癌标本28例、肾透明细胞癌标本32例和单纯性肾囊肿标本13例,采用免疫组化方法检测CD2AP在上述组织中的表达.从上述3组组织蜡块中每组各随机抽取5例共15例,重新切片,用CD2AP和肾质,做免疫组化染色对照实验.结果在多房囊性肾细胞癌的肿瘤细胞中CD2AP表达较强,尤其在囊壁的内层上皮表达强度明显增加.CD2AP在肾透明细胞癌中有弱表达,在单纯性肾囊肿中没有表达.肾质和CD2AP在3种组织的对照实验结果中染色强度和部位完全一致.结论 CD2AP在多房囊性肾细胞癌中的表达具有特异性,其表达与肾透明细胞癌和单纯性肾囊肿有显著差异,对于多房囊性肾细胞癌的鉴别诊断有重要意义.CD2AP和肾质在多房囊性肾细胞癌中分布一致的特点,以及上述两种蛋白的足细胞特异性对于多房囊性肾细胞癌的形成原因具有重要提示.
목적 탐토CD2상관단백(CD2AP)급신질(nephrin)재성인다방낭성신세포암중적표체급의의.방법 수집다방낭성신세포암표본28례、신투명세포암표본32례화단순성신낭종표본13례,채용면역조화방법 검측CD2AP재상술조직중적표체.종상술3조조직사괴중매조각수궤추취5례공15례,중신절편,용CD2AP화신질,주면역조화염색대조실험.결과 재다방낭성신세포암적종류세포중CD2AP표체교강,우기재낭벽적내층상피표체강도명현증가.CD2AP재신투명세포암중유약표체,재단순성신낭종중몰유표체.신질화CD2AP재3충조직적대조실험결과 중염색강도화부위완전일치.결론 CD2AP재다방낭성신세포암중적표체구유특이성,기표체여신투명세포암화단순성신낭종유현저차이,대우다방낭성신세포암적감별진단유중요의의.CD2AP화신질재다방낭성신세포암중분포일치적특점,이급상술량충단백적족세포특이성대우다방낭성신세포암적형성원인구유중요제시.
Objective To investigate the role of CD2-associated protein(cD2AP)and nephrin expression in the muhilocular cystic renal cell carcinoma(MCRCC)Methods Seventy four specimens of renal lesions.including 29 cases of MCRCC,32 case of clear cell renal cell carcinoma(CCRCC),and 13 cases of simple renal cyst(SRC),were collected during operation.Immunohistochemistry was used to detect tlle CD2AP expression.Five wax lumps from each of the 3 groups of specimen,totally 15,were randomly selected to undergo re-sectioning and immunohistochemistry so as to compare the levels of staining intensity and location of expression of CD2AP and nephrin in the same sections.Results The expression of CD2AP in the MCRCC specimens was stronger than that in the CCRCC that was in turn stronger tllan that than that in the simple renal cyst(SRC)specimens.The epithelium lining the cysts in MCRCC was strongly positive in CD2AP compared to other parts.CCRCC showed a weak expression of CD2AP,while CD2AP Was negative in the SRC specimens.The staining intensity and location of expression were completely identieal for nephrin and CD2AP among the three types of tissue specimens.ConclusiOIlS The CD2AP expression iS much stronger in MCRCC than in CCRCC and SRC.CD2AP may be used as a biomarker in differential diagnosis of MCRCC.CCRCC.and SRC.The identical expression of CD2AP and nephrin in MCRCC and their specific expression in podocytes are important hints for the mechanism of MCRCC.