CAJ | 학술논문

目的研究牡芍合剂对骨折术后疼痛模型大鼠丘脑中β-内啡肽(β-EP)及八肽胆囊收缩素(CCK-8)影响的时间依赖性,探讨牡芍合剂术后应用的时间.方法选取体质量250~300 g SPF级雄性Wistar大鼠随机分为:正常对照组(空白组)、模型组、牡芍合剂治疗组和曲马多对照组.采用Brennan术后痛模型,术后曲马多对照组、治疗组立即给药,给药后分别于1 h、24 h、48 h处死动物取脊髓,采用放射免疫法测定β-EP及CCK-8的含量.结果与模型组相比,牡芍合剂能显著升高术后疼痛模型大鼠丘脑中β-EP含量,且随着用药时间的增加其促进β-内啡肽释放的作用逐渐加强;同时它可降低CCK-8的含量,此作用大小与用药时间无关.结论牡芍合剂可以促进β-EP的分泌,且有时间依赖性;同时牡芍合剂可以抑制CCK-8的分泌,无时间依赖性.
목적 연구모작합제대골절술후동통모형대서구뇌중β-내배태(β-EP)급팔태담낭수축소(CCK-8)영향적시간의뢰성,탐토모작합제술후응용적시간.방법 선취체질량250~300 g SPF급웅성Wistar대서수궤분위:정상대조조(공백조)、모형조、모작합제치료조화곡마다대조조.채용Brennan술후통모형,술후곡마다대조조、치료조립즉급약,급약후분별우1 h、24 h、48 h처사동물취척수,채용방사면역법측정β-EP급CCK-8적함량.결과 여모형조상비,모작합제능현저승고술후동통모형대서구뇌중β-EP함량,차수착용약시간적증가기촉진β-내배태석방적작용축점가강;동시타가강저CCK-8적함량,차작용대소여용약시간무관.결론 모작합제가이촉진β-EP적분비,차유시간의뢰성;동시모작합제가이억제CCK-8적분비,무시간의뢰성.
Objective To study the chronergy of effect of Mushao composition on β-endorphin (β-EP) and cholecystokinin-octopeptide (CCK-8) in the thalamencephals of rat models of bone fracture with postoperative ache, and to explore the opportunity of Mushao composition administration after fracture operation.Methods Ninety-six healthy male Wistar rats of SPF level weighing 250-300 g were randomly divided into healthy control group (blank group), model group, therapy group and tramadol control group. The rat model of Brennan postoperative ache was developed. The corresponding agent was given immediately after operation in therapy group and tramadol control group. The animals were sacrificed so as to collect the spinal cord samples at 1 h, 24 h, 48 h timepoint after operation. The contents of β-EP and CCK-8 were measured by applying radioimmunoassay. Results Compared with model group, Mushao composition significantly increased the level of the β-EP in the thalamencephal, and the content of β-EP was increased with time; while it could decrease the level of CCK-8, which wasn′t associated with the time of medication.Conclusion Mushao composition can increase the content of β-EP in time-depedent manner, and while it can inhibit the secretion of CCK-8, which isn′t associated with the time of medication.