绍兴文理学院学报
紹興文理學院學報
소흥문이학원학보
Journal of Shaoxing University
2009年
8期
72-77
,共6页
钱伟平%易明花%孙小红%王伟娣
錢偉平%易明花%孫小紅%王偉娣
전위평%역명화%손소홍%왕위제
麦冬%块根%须根%水培诱导根%显微结构
麥鼕%塊根%鬚根%水培誘導根%顯微結構
맥동%괴근%수근%수배유도근%현미결구
ophiopogon japonicus%root tuber%fibrous root%root cultured in water%cell and tissue structures in optical microscopy
采用组织切片技术,在显微镜下观察麦冬块根、须根和水培诱导根的形态和内部结构.研究麦冬块根、须根和水培诱导根的组织结构和细胞结构的差异.显微镜下的观察显示:在麦冬块根的皮层细胞中看到胶冻样特征性结构.麦冬须根的皮层薄壁组织之间排列着草酸钙针晶柱长链,须根的根尖区有密集的草酸钙针晶束结构;水培诱导根中没有发现草酸钙针晶束结构.水培诱导根的根毛与须根的根毛相比较,管腔内的无定型物质要比须根根毛少得多.研究结果表明:麦冬的块根从须根分化之后,在植物生理机理上与须根存在着差异性.正是这种机能差异,才形成了麦冬块根的药用有效成分的细胞生物学基础.草酸钙是由麦冬的须根根尖从土壤中吸收而来,或者是吸收了土壤中的钙离子,在根尖部位进行生化反应进行合成,再通过须根的皮层中央薄壁组织向上输送,途经块根有所残留.
採用組織切片技術,在顯微鏡下觀察麥鼕塊根、鬚根和水培誘導根的形態和內部結構.研究麥鼕塊根、鬚根和水培誘導根的組織結構和細胞結構的差異.顯微鏡下的觀察顯示:在麥鼕塊根的皮層細胞中看到膠凍樣特徵性結構.麥鼕鬚根的皮層薄壁組織之間排列著草痠鈣針晶柱長鏈,鬚根的根尖區有密集的草痠鈣針晶束結構;水培誘導根中沒有髮現草痠鈣針晶束結構.水培誘導根的根毛與鬚根的根毛相比較,管腔內的無定型物質要比鬚根根毛少得多.研究結果錶明:麥鼕的塊根從鬚根分化之後,在植物生理機理上與鬚根存在著差異性.正是這種機能差異,纔形成瞭麥鼕塊根的藥用有效成分的細胞生物學基礎.草痠鈣是由麥鼕的鬚根根尖從土壤中吸收而來,或者是吸收瞭土壤中的鈣離子,在根尖部位進行生化反應進行閤成,再通過鬚根的皮層中央薄壁組織嚮上輸送,途經塊根有所殘留.
채용조직절편기술,재현미경하관찰맥동괴근、수근화수배유도근적형태화내부결구.연구맥동괴근、수근화수배유도근적조직결구화세포결구적차이.현미경하적관찰현시:재맥동괴근적피층세포중간도효동양특정성결구.맥동수근적피층박벽조직지간배렬착초산개침정주장련,수근적근첨구유밀집적초산개침정속결구;수배유도근중몰유발현초산개침정속결구.수배유도근적근모여수근적근모상비교,관강내적무정형물질요비수근근모소득다.연구결과표명:맥동적괴근종수근분화지후,재식물생리궤리상여수근존재착차이성.정시저충궤능차이,재형성료맥동괴근적약용유효성분적세포생물학기출.초산개시유맥동적수근근첨종토양중흡수이래,혹자시흡수료토양중적개리자,재근첨부위진행생화반응진행합성,재통과수근적피층중앙박벽조직향상수송,도경괴근유소잔류.
The LEICA RM2128 slicer and Motic BA300 optical microscopy system has been used to observe the cell and tissue structures of root tubers,fibrous roots and the roots cultured in water of O.japonicus with a view to investigating the differences of the respective cell and tissue structures.The results show that there are many gelatum-like structures in the cortical cells of the root tuber.There are several chains which contain Calcium Oxalic acid in the cortical parenchyma of the fibrous root and much more serried at its tip.In the root cultured in water,little Calcium Oxalic acid is found.The comparison between the fibril of the root cultured in water and the fibrous root indicates that amorphous substances located in the cavity of the former is fewer that those of the latter.The paper arrives at the conclusion:When the fibrous root of O.japonicus turns into the root tuber,the function of cell and tissue mechanism has changed.This change acts on the cell level,resulting in the herb utilization of the fibrous root of O.japonicus.The Calcium Oxalic acid is absorbed from the soil or is synthesized at the tip of a fibrous root through a certain chemical reaction.Then it is transmitted along the parenchyma in the fibrous root and some of them is stored in the root tuber of O.japonicus.