CAJ | 학술논문

介绍了一种利用金胶的选择性聚集实现信号扩增的超灵敏的电化学方法, 用于人类p53肿瘤抑制剂基因的检测. 在实验中, 根据p53基因的序列设计了能特异性检测p53肿瘤抑制剂基因的二段探针, 在一段探针上固定磁性颗粒以捕获并富集目标基因, 同时在另一段探针上标记金纳米颗粒作为检测信标. 另外, 通过硫代三聚氰酸和金纳米颗粒的自组装作用, 形成金纳米颗粒和硫代三聚氰酸的网状结构, 获得金纳米颗粒的选择性聚集, 实现信号扩增. 用此法检测目标p53野生型DNA, 最低检测限为2.24×10-17 mol/L, 同时进一步研究了该探针对p53野生型和一碱基错配的突变型的选择性.
개소료일충이용금효적선택성취집실현신호확증적초령민적전화학방법, 용우인류p53종류억제제기인적검측. 재실험중, 근거p53기인적서렬설계료능특이성검측p53종류억제제기인적이단탐침, 재일단탐침상고정자성과립이포획병부집목표기인, 동시재령일단탐침상표기금납미과립작위검측신표. 령외, 통과류대삼취청산화금납미과립적자조장작용, 형성금납미과립화류대삼취청산적망상결구, 획득금납미과립적선택성취집, 실현신호확증. 용차법검측목표p53야생형DNA, 최저검측한위2.24×10-17 mol/L, 동시진일보연구료해탐침대p53야생형화일감기착배적돌변형적선택성.
An ultrasensitive and high specific electrochemical assay for the detection of human tumor suppression p53 gene has been developed. Two DNA sequences were designed, and the combined sequences were complementary to that of the target p53 sequence, one was immobilized on the magnetic nanoparticles for capturing the target p53 sequence, the other was labeled with gold nanoparticles as probe. The ultrasensitive detection was achieved by the aggregation of gold nanoparticles with introducing thiocyanuric acid into the system to form the network like structure, and the detection limit could go down to 2.24×10~(-17) mol/L for the wild type p53 sequence. The discrimination between the mutant type p53 DNA and the wide type p53 DNA was studied with the proposed assay.