细胞与分子免疫学杂志
細胞與分子免疫學雜誌
세포여분자면역학잡지
2009年
10期
927-928,931
,共3页
慢性淋巴细胞性甲状腺炎%辅助性T细胞17%流式细胞术
慢性淋巴細胞性甲狀腺炎%輔助性T細胞17%流式細胞術
만성림파세포성갑상선염%보조성T세포17%류식세포술
chronic lymphocytic thyroiditis%T help 17 cells%flow cytometry
目的:探讨慢性淋巴细胞性甲状腺炎(CLT)患者外周血辅助性T细胞17(Th17)的水平及意义.方法:收集甲状腺功能正常组CLT患者15例,甲状腺功能减退组CLT患者30例及20例健康对照组研究者的外周血样.采用胞内细胞因子染色流式细胞术(FCM)分析法,检测各组外周血Th17细胞占CD4~+T细胞比值.应用电化学发光法检测CLT患者及健康对照组血清中抗甲状腺过氧化物酶抗体(TPO-Ab)和抗甲状球蛋白抗体(TG-Ab)水平.结果:与健康对照组比较:甲状腺功能正常组及甲状腺功能减退组的CLT患者外周血Th17/CD4~+T细胞比值分别为(3.64±1.96)%、(3.13±1.56)%,均高于健康对照组(0.50±0.32%,P<0.01);两组CLT患者血清TPO-Ab和TG-Ab水平分别为(398.99±222.88;1456.02±1054.97)IU/L和(423.26±167.21;1587.94±1210.36)IU/L,均明显高于健康对照组(15.23±10.36;36.32±26.99)IU/L;差异有统计学意义(P<0.01).CLT患者外周血Th17/CD4+T细胞比值改变与血清TPO-Ab及TG-Ab阳性表达呈显著正相关(分别为r=0.50,r=0.43;P<0.01).结论:两组CLT患者外周血Th17细胞比率增加,且与甲状腺自身抗体水平存在显著正相关,Th17细胞可能参与CLT的发生发展.
目的:探討慢性淋巴細胞性甲狀腺炎(CLT)患者外週血輔助性T細胞17(Th17)的水平及意義.方法:收集甲狀腺功能正常組CLT患者15例,甲狀腺功能減退組CLT患者30例及20例健康對照組研究者的外週血樣.採用胞內細胞因子染色流式細胞術(FCM)分析法,檢測各組外週血Th17細胞佔CD4~+T細胞比值.應用電化學髮光法檢測CLT患者及健康對照組血清中抗甲狀腺過氧化物酶抗體(TPO-Ab)和抗甲狀毬蛋白抗體(TG-Ab)水平.結果:與健康對照組比較:甲狀腺功能正常組及甲狀腺功能減退組的CLT患者外週血Th17/CD4~+T細胞比值分彆為(3.64±1.96)%、(3.13±1.56)%,均高于健康對照組(0.50±0.32%,P<0.01);兩組CLT患者血清TPO-Ab和TG-Ab水平分彆為(398.99±222.88;1456.02±1054.97)IU/L和(423.26±167.21;1587.94±1210.36)IU/L,均明顯高于健康對照組(15.23±10.36;36.32±26.99)IU/L;差異有統計學意義(P<0.01).CLT患者外週血Th17/CD4+T細胞比值改變與血清TPO-Ab及TG-Ab暘性錶達呈顯著正相關(分彆為r=0.50,r=0.43;P<0.01).結論:兩組CLT患者外週血Th17細胞比率增加,且與甲狀腺自身抗體水平存在顯著正相關,Th17細胞可能參與CLT的髮生髮展.
목적:탐토만성림파세포성갑상선염(CLT)환자외주혈보조성T세포17(Th17)적수평급의의.방법:수집갑상선공능정상조CLT환자15례,갑상선공능감퇴조CLT환자30례급20례건강대조조연구자적외주혈양.채용포내세포인자염색류식세포술(FCM)분석법,검측각조외주혈Th17세포점CD4~+T세포비치.응용전화학발광법검측CLT환자급건강대조조혈청중항갑상선과양화물매항체(TPO-Ab)화항갑상구단백항체(TG-Ab)수평.결과:여건강대조조비교:갑상선공능정상조급갑상선공능감퇴조적CLT환자외주혈Th17/CD4~+T세포비치분별위(3.64±1.96)%、(3.13±1.56)%,균고우건강대조조(0.50±0.32%,P<0.01);량조CLT환자혈청TPO-Ab화TG-Ab수평분별위(398.99±222.88;1456.02±1054.97)IU/L화(423.26±167.21;1587.94±1210.36)IU/L,균명현고우건강대조조(15.23±10.36;36.32±26.99)IU/L;차이유통계학의의(P<0.01).CLT환자외주혈Th17/CD4+T세포비치개변여혈청TPO-Ab급TG-Ab양성표체정현저정상관(분별위r=0.50,r=0.43;P<0.01).결론:량조CLT환자외주혈Th17세포비솔증가,차여갑상선자신항체수평존재현저정상관,Th17세포가능삼여CLT적발생발전.
AIM: To investigate the alteration and its significance of T help 17 cells(Th17) in patients with chronic lymphocytic thyroiditis(CLT). METHODS: Patients were divided into 3 groups: CLT patients with euthyroidism (n = 15), CLT patients with hypothyroidism (n = 30) and healthy control group(n = 20). The ratio of Th17 lymphocytes subpopulations in the preipheral blood were evaluated by technique of flow cytometry. Production of thyroid autoantibody (TPO-Ab, TG-Ab) were measured by electro-chemiluminescence immunoassay (ECLIA). RESULTS: Compared with the healthy control group, in CLT group: The frequencies of Th17 in peripheral blood were found to be significantly higher in patients with CLT than healthy control group (P < 0.01); Production of TPO-Ab and TG-Ab markedly increased in CLT patients than healthy control group (P < 0.01). There was significant correlation between the positive expression of thyroid autoantibody and the changes of Th17 subpopulations (r=0.50, r=0.43 respectively; P < 0.01). CONCLUSION: The frequencies of Th17 cell increased in patients with CLT which may suggest a potential role for Th17 in the progression and happen of CLT.
