CAJ | 학술논문

[目的]研究与紫萍半叶状体衰老相关的内肽酶及其性质.[方法]采用明胶-SDS-聚丙烯酰胺凝胶电泳技术分析与衰老相关的内肽酶同工酶,并用蛋白酶抑制剂分析内肽酶类型.[结果]对紫萍衰老期间内肽酶的分析表明,共有6种内肽酶出现,金属型蛋白酶HEP1和半胱氨酸型内肽酶HEP2、HEP4、HEP6是与衰老相关的内肽酶. [结论] 在紫萍半叶状体衰老早期金属型内肽酶活性较强,而在衰老后期半胱氨酸型内肽酶大量出现并具有较高的活性.
[목적]연구여자평반협상체쇠로상관적내태매급기성질.[방법]채용명효-SDS-취병희선알응효전영기술분석여쇠로상관적내태매동공매,병용단백매억제제분석내태매류형.[결과]대자평쇠로기간내태매적분석표명,공유6충내태매출현,금속형단백매HEP1화반광안산형내태매HEP2、HEP4、HEP6시여쇠로상관적내태매. [결론] 재자평반협상체쇠로조기금속형내태매활성교강,이재쇠로후기반광안산형내태매대량출현병구유교고적활성.
[Objective] The aim was to study endopeptidases in Spirodela polyrrhiza half-fronds during senescence. Furthermore, the characters of senescence-related endopeptidase were also investigated. [Method] Changes in endopeptidase isoenzymes of Spirodela polyrrhiza half-fronds during senescence were investigated by gelatin-SDS-PAGE electrophoresis and protease inhibitors. [Result]The results showed that six endopeptidases were detected in half-fronds. HEP1,HEP2, HEP4, HEP6 were senescence-related endopeptidases. [Conclusion]The metallo-endopeptidase play significant roles in early stage and the cysteine endopeptidase were the most abundant endopeptidase during the late stage of senescence.