CAJ | 학술논문

建立白芍商品药材指纹图谱,并测定芍药苷含量评价药材质量.采用高效液相色谱分析,Hypersil ODS C_(18)柱(4.6mm×250mm,5μm),乙腈-0.08%磷酸溶液梯度洗脱,柱温30℃.测定了市售17批白芍药材的HPLC指纹图谱,建立了白芍药材的总共有模式,计算得到各批药材的相似度,其中8批药材的相似度介于0.902-0.985.以相似度0.90为界,大于0.90认定为合格药材,小于0.90为不合格药材.本评价方法的结果与根据药典标准的划分结果一致,但更直观,可操作性更强,尤其对白芍药材中硫化物进行有效监控,还可对熏硫药材的质量控制起到示范作用.
건립백작상품약재지문도보,병측정작약감함량평개약재질량.채용고효액상색보분석,Hypersil ODS C_(18)주(4.6mm×250mm,5μm),을정-0.08%린산용액제도세탈,주온30℃.측정료시수17비백작약재적HPLC지문도보,건립료백작약재적총공유모식,계산득도각비약재적상사도,기중8비약재적상사도개우0.902-0.985.이상사도0.90위계,대우0.90인정위합격약재,소우0.90위불합격약재.본평개방법적결과여근거약전표준적화분결과일치,단경직관,가조작성경강,우기대백작약재중류화물진행유효감공,환가대훈류약재적질량공제기도시범작용.
A HPLC-fingerprint of Radix Paeoniae Alba was established and the drug's quality was evaluated by determining the content of paeoniflorin.Analysis was performed using a Hypersil ODS C_(18) column (4.6mm×250mm, 5μm) with the mixture of acetonitrile-0.08% phosphoric acid solution as mobile phase in gradient mode, and the column temperature was at 30℃.Seventeen samples of the crude drugs of Radix Paeoniae Alba were determined.The mutual model was established and the similarity degrees were calculated.Similarity degrees of 8 samples were between 0.902-0.985 respectively.The limit of similarity degree for Radix Paeoniae Alba was set at 0.90.If the similarity degree was greater than 0.90,it was qualified crude drug,otherwise it was unqualified.The evaluation results from this method were the same as the results from the method published in the Chinese Pharmacopoeia (2010ed.) and it can be used to control the sulfide contended in Radix Paeoniae Alba.