CAJ | 학술논문

目的通过检测子痫前期患者β-纤维蛋白原-455基因,分析其基因多态性及子痫前期发生的关系.方法利用聚合酶链反应,限制型长度多态性对2007年1至12月在广东省妇幼保健院分娩的96例子痫前期患者和98例正常孕妇进行不同β-纤维蛋白原-455G/A基因型分析,并观察两组血浆纤维蛋白原情况.结果子痫前期患者血浆纤维蛋白原水平显著高于正常妊娠者(P＜0.05).B-FIB-455G/A基因多态性GA+AA等位基因携带者血浆纤维蛋白原水平明显高于GG等位基因者(P＜0.05),可见A等位基因与高纤维蛋白原水平相关.A等位基因频率及GA、AA基因型在子痫前期组和对照组间无明显区别(P＞0.05).结论 β-FIB-455G/A基因多态性A等位基因可能不是子痫前期的遗传易感基因,但与子痫前期患者血浆高纤维蛋白原水平相关.
목적 통과검측자간전기환자β-섬유단백원-455기인,분석기기인다태성급자간전기발생적관계.방법 이용취합매련반응,한제형장도다태성대2007년1지12월재광동성부유보건원분면적96례자간전기환자화98례정상잉부진행불동β-섬유단백원-455G/A기인형분석,병관찰량조혈장섬유단백원정황.결과 자간전기환자혈장섬유단백원수평현저고우정상임신자(P＜0.05).B-FIB-455G/A기인다태성GA+AA등위기인휴대자혈장섬유단백원수평명현고우GG등위기인자(P＜0.05),가견A등위기인여고섬유단백원수평상관.A등위기인빈솔급GA、AA기인형재자간전기조화대조조간무명현구별(P＞0.05).결론 β-FIB-455G/A기인다태성A등위기인가능불시자간전기적유전역감기인,단여자간전기환자혈장고섬유단백원수평상관.
Objective To evaluate the polymorphism of different alleles in β-455G/A gene,the distributive characteristics in pre-eclampsia patients and normal pregnant women,and the relationship of these genetic variants with plasma fibfinogen levels.Methods 96 pre-eclampsia women,and 98 normal pregnant women,their plasma levels of fibrinogen were determined.Polymorphisms of β-455G/A gene were measured by polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) and Hha Ⅲ endonuclease digestion and Polyacrylamide Gel Electrophoresis(PAGE).Results Comparing the plasma fibrinogen level,a significantly plasma fibrinogen concentration was founded in re-eclampsia group(P＜0.05).Comparing the plasma fibrinogen level between the different genotype group,a significantly higher plasma fibrinogen level in the β-FIB-455G/AGA+AA group was detected(P＜0.05).There was no significant difference in the distribution of the Bβ-FIB-455G/A gene between two groups.Conclusions Allele distribution of Bβ-FIB-455G/A was significantly associated with plasma fibrinogen level.The variation frequency of fibrinogen β-455G/A genotype was not associated with the occurrence of pre-eclampsia.