中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2011年
11期
1060-1065
,共6页
卢遥%王静成%顾加祥%颜连启%冯新民%戴善和%王强%虞海流%解慧琪%李秀群%杨志明
盧遙%王靜成%顧加祥%顏連啟%馮新民%戴善和%王彊%虞海流%解慧琪%李秀群%楊誌明
로요%왕정성%고가상%안련계%풍신민%대선화%왕강%우해류%해혜기%리수군%양지명
间质干细胞%胎盘%骨髓%组织工程%兔
間質榦細胞%胎盤%骨髓%組織工程%兔
간질간세포%태반%골수%조직공정%토
Mesenchymal stem cells%Placenta%Bone marrow%Tissue engineering%Rabbit
目的 探讨兔胎盘来源间充质干细胞(PMSCs)构建的组织工程化骨修复兔桡骨节段性骨缺损的能力.方法 取24只大耳白兔,于兔双侧桡骨中下段制造1.5cm的节段性骨缺损,左侧骨缺损用PMSCs构建的组织工程化骨桥接(实验组),右侧用骨髓基质干细胞(BMSCs)构建的组织工程化骨桥接(对照组).实验动物双侧于术后2、4、8、12周摄X线片和取材,对双侧X线片进行影像学评分；对取材行大体、组织学检查、生物力学性能测定和扫描电镜观察.结果 术后2、4、8、12周实验组与对照组X线评分平均分别为(3.72±0.24)和(3.49±0.29)分、(6.24 ±0.41)和(5.91±0.45)分、(8.15±0.67)和(8.87±0.59)分、(1139±0.86)和(12.04±0.93)分,两组比较差异均无统计学意义(P＞0.05).组织学观察显示膜内化骨和软骨内化骨均参与了双侧骨缺损愈合的成骨过程,但以软骨内化骨为主.术后2、4、8、12周实验组与对照组最大载荷平均分别为(48.76±6.03)和(56.17±4.10)N、(65.64±4.49)和(74.67±7.69)N、(101.04±3.35)和(90.77±6.91)N、(131.35±6.91)和(150.61±21.41)N,两组比较差异均无统计学意义(P＞0.05).结论 两种细胞构建的组织工程化骨都可以较快地修复长骨临界大小节段性骨缺损,PMSCs与BMSCs具有相似的生物学特性和成骨特性,可作为骨组织工程的另一成体干细胞来源.
目的 探討兔胎盤來源間充質榦細胞(PMSCs)構建的組織工程化骨脩複兔橈骨節段性骨缺損的能力.方法 取24隻大耳白兔,于兔雙側橈骨中下段製造1.5cm的節段性骨缺損,左側骨缺損用PMSCs構建的組織工程化骨橋接(實驗組),右側用骨髓基質榦細胞(BMSCs)構建的組織工程化骨橋接(對照組).實驗動物雙側于術後2、4、8、12週攝X線片和取材,對雙側X線片進行影像學評分；對取材行大體、組織學檢查、生物力學性能測定和掃描電鏡觀察.結果 術後2、4、8、12週實驗組與對照組X線評分平均分彆為(3.72±0.24)和(3.49±0.29)分、(6.24 ±0.41)和(5.91±0.45)分、(8.15±0.67)和(8.87±0.59)分、(1139±0.86)和(12.04±0.93)分,兩組比較差異均無統計學意義(P＞0.05).組織學觀察顯示膜內化骨和軟骨內化骨均參與瞭雙側骨缺損愈閤的成骨過程,但以軟骨內化骨為主.術後2、4、8、12週實驗組與對照組最大載荷平均分彆為(48.76±6.03)和(56.17±4.10)N、(65.64±4.49)和(74.67±7.69)N、(101.04±3.35)和(90.77±6.91)N、(131.35±6.91)和(150.61±21.41)N,兩組比較差異均無統計學意義(P＞0.05).結論 兩種細胞構建的組織工程化骨都可以較快地脩複長骨臨界大小節段性骨缺損,PMSCs與BMSCs具有相似的生物學特性和成骨特性,可作為骨組織工程的另一成體榦細胞來源.
목적 탐토토태반래원간충질간세포(PMSCs)구건적조직공정화골수복토뇨골절단성골결손적능력.방법 취24지대이백토,우토쌍측뇨골중하단제조1.5cm적절단성골결손,좌측골결손용PMSCs구건적조직공정화골교접(실험조),우측용골수기질간세포(BMSCs)구건적조직공정화골교접(대조조).실험동물쌍측우술후2、4、8、12주섭X선편화취재,대쌍측X선편진행영상학평분；대취재행대체、조직학검사、생물역학성능측정화소묘전경관찰.결과 술후2、4、8、12주실험조여대조조X선평분평균분별위(3.72±0.24)화(3.49±0.29)분、(6.24 ±0.41)화(5.91±0.45)분、(8.15±0.67)화(8.87±0.59)분、(1139±0.86)화(12.04±0.93)분,량조비교차이균무통계학의의(P＞0.05).조직학관찰현시막내화골화연골내화골균삼여료쌍측골결손유합적성골과정,단이연골내화골위주.술후2、4、8、12주실험조여대조조최대재하평균분별위(48.76±6.03)화(56.17±4.10)N、(65.64±4.49)화(74.67±7.69)N、(101.04±3.35)화(90.77±6.91)N、(131.35±6.91)화(150.61±21.41)N,량조비교차이균무통계학의의(P＞0.05).결론 량충세포구건적조직공정화골도가이교쾌지수복장골림계대소절단성골결손,PMSCs여BMSCs구유상사적생물학특성화성골특성,가작위골조직공정적령일성체간세포래원.
Objective To compare osteogenic capacities of allogeneic bone marrow stromal cells (BMSCs)and planceta marrow stronal cells(PMSCs)to repair critical-sized segmental bone defects in rabbits.Methods Segmental osteoperiosteal defects of 1.5 cm were created surgically at bilateral radii in 24 rabbits.The defects at the left side were bridged with tissue engineered bone constructed by PMSCs (experimental group)while those at the right side were bridged with tissue engineered bone constructed by BMSCs(control group).The rabbits were observed generally after the operation.At 2,4,8,12 weeks postoperatively,X-rays of the bilateral radii were taken for radiographic assessments and the animals were sacrificed to harvest the involved radii.After macroscopic examination,the defects and their adjacent bone were prepared for histological and scanning electron microscopy observations as well as for biomechanical tests.Results The radiographic scores at 2,4,8,12 weeks postoperatively for the experimental and control groups were respectively 3.72 ±0.24 versus 3.49 ±0.29,6.24 ±0.41 versus 5.91 ±0.45,8.15 ±O.67 versus 8.87 ± O.59,and 11.39 ± 0.86 versus 12.04 ± 0.93,with no significant differences(P ＞0.05).Both intramembranous and endochondral ossifications were involved in the osteogenic processes of the bone defect repair,but the latter doninated the process.The maximum loads at 2,4,8,12 weeks postoperatively for the experimental and control groups were respectively 48.76 ± 6.03 N versus 56.17 ±4.10 N,65.64±4.49 N versus 74.67±7.69 N,101.04±3.35 N versus 90.77±6.91 N,and 131.35±6.91 N versus 150.61 ± 21.41 N,with no significant differences(P ＞ 0.05).Conclusions Tissue engineered bone constructed with either allogeneic PMSCs or BMSCs is capable of healing critical-sized segmental bone defects in rabbits.Since PMSCs have similar biological characteristics and osteogenic capacity compared with BMSCs,they can be used as a new source of seeding cells in bone tissue engineering.
