中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2008年
11期
830-834
,共5页
毛敏%张建国%陶爱林%严小玲%欧阳顺林%袁旭平%丘理子%谢洋%邹泽红%卢洁贞%李青
毛敏%張建國%陶愛林%嚴小玲%歐暘順林%袁旭平%丘理子%謝洋%鄒澤紅%盧潔貞%李青
모민%장건국%도애림%엄소령%구양순림%원욱평%구리자%사양%추택홍%로길정%리청
老年性聋%模型,动物%诱发电位,听觉,脑干%多态性,扩增性片段长度
老年性聾%模型,動物%誘髮電位,聽覺,腦榦%多態性,擴增性片段長度
노년성롱%모형,동물%유발전위,은각,뇌간%다태성,확증성편단장도
Presbycusis%Models,animal%Evoked potentials,auditory,brain stem%Polymorphism,amplified fragment length
目的 建立D-半乳糖致豚鼠老化模型,应用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)分析检测与听力损失相关的分子生物学标记,寻求老年性聋发病的分子机制.方法 51只豚鼠随机分为三组:A组(模型实验组)21只,5%D-半乳糖腹腔注射(200 mg·kd-1·d-1),共6周;B组(模型对照组)15只,仅给予生理盐水注射.A、B两组注射前后分别进行听性脑干反应(ABR)检测,将两组豚鼠置于噪声环境中7 d,每天噪声暴露8 h,结束后再次检测ABR阈值.C组(空白对照组)15只,不加任何处理,仅予以同步检测ABR阈值.用比色法检测三组肝和脑组织的超氧化物歧化酶(superoxide dismutase,SOD)及丙二醛(maleic dialdehyde,MDA)含量.提取三组豚鼠内耳组织DNA,应用AFLP技术筛选差异位点.结果 注射后A组豚鼠ABR阈值较B组提高,但两组阈移之间差异无统计学意义(t=1.14,P>0.05),噪声暴露后,A组ABR阈值(峰等效声压级)平均提高(22.97±10.56)dB,B组提高(14.16±7.36)dB,组间差异具有统计学意义(t=2.78,P<0.05).A组肝和脑组织中SOD活性明显低于B组,MDA含最则明显高于B组,其差异具有统计学意义(P值均<0.01).AFLP分析发现有与耳聋一致的多态性标记.结论 D-半乳糖可以诱导豚鼠衰老,此模型豚鼠听反应阈虽无明显提高,但对噪声的敏感性增加,AFLP检测到的差异位点可能与其对噪声敏感有关.
目的 建立D-半乳糖緻豚鼠老化模型,應用擴增片段長度多態性(amplified fragment length polymorphism,AFLP)分析檢測與聽力損失相關的分子生物學標記,尋求老年性聾髮病的分子機製.方法 51隻豚鼠隨機分為三組:A組(模型實驗組)21隻,5%D-半乳糖腹腔註射(200 mg·kd-1·d-1),共6週;B組(模型對照組)15隻,僅給予生理鹽水註射.A、B兩組註射前後分彆進行聽性腦榦反應(ABR)檢測,將兩組豚鼠置于譟聲環境中7 d,每天譟聲暴露8 h,結束後再次檢測ABR閾值.C組(空白對照組)15隻,不加任何處理,僅予以同步檢測ABR閾值.用比色法檢測三組肝和腦組織的超氧化物歧化酶(superoxide dismutase,SOD)及丙二醛(maleic dialdehyde,MDA)含量.提取三組豚鼠內耳組織DNA,應用AFLP技術篩選差異位點.結果 註射後A組豚鼠ABR閾值較B組提高,但兩組閾移之間差異無統計學意義(t=1.14,P>0.05),譟聲暴露後,A組ABR閾值(峰等效聲壓級)平均提高(22.97±10.56)dB,B組提高(14.16±7.36)dB,組間差異具有統計學意義(t=2.78,P<0.05).A組肝和腦組織中SOD活性明顯低于B組,MDA含最則明顯高于B組,其差異具有統計學意義(P值均<0.01).AFLP分析髮現有與耳聾一緻的多態性標記.結論 D-半乳糖可以誘導豚鼠衰老,此模型豚鼠聽反應閾雖無明顯提高,但對譟聲的敏感性增加,AFLP檢測到的差異位點可能與其對譟聲敏感有關.
목적 건립D-반유당치돈서노화모형,응용확증편단장도다태성(amplified fragment length polymorphism,AFLP)분석검측여은력손실상관적분자생물학표기,심구노년성롱발병적분자궤제.방법 51지돈서수궤분위삼조:A조(모형실험조)21지,5%D-반유당복강주사(200 mg·kd-1·d-1),공6주;B조(모형대조조)15지,부급여생리염수주사.A、B량조주사전후분별진행은성뇌간반응(ABR)검측,장량조돈서치우조성배경중7 d,매천조성폭로8 h,결속후재차검측ABR역치.C조(공백대조조)15지,불가임하처리,부여이동보검측ABR역치.용비색법검측삼조간화뇌조직적초양화물기화매(superoxide dismutase,SOD)급병이철(maleic dialdehyde,MDA)함량.제취삼조돈서내이조직DNA,응용AFLP기술사선차이위점.결과 주사후A조돈서ABR역치교B조제고,단량조역이지간차이무통계학의의(t=1.14,P>0.05),조성폭로후,A조ABR역치(봉등효성압급)평균제고(22.97±10.56)dB,B조제고(14.16±7.36)dB,조간차이구유통계학의의(t=2.78,P<0.05).A조간화뇌조직중SOD활성명현저우B조,MDA함최칙명현고우B조,기차이구유통계학의의(P치균<0.01).AFLP분석발현유여이롱일치적다태성표기.결론 D-반유당가이유도돈서쇠로,차모형돈서은반응역수무명현제고,단대조성적민감성증가,AFLP검측도적차이위점가능여기대조성민감유관.
Objective To explore the establishment of the mimetic aging effect in guinea pigs induced by D-galactose, and to detect the biological indicatrix associated with hearing loss and provide a new tool for molecular pathogenesis of hearing loss.Methods Total of 51 guinea pigs were randomly divided into three groups: group A ( model aging group , n = 25) , which were injected with D-galactose (200 mg ·kg-1·d-1 ) by intra peritoneum for 6 weeks, group B ( model control group , n = 18), which were given the same amount of saline only, and group C(vacant group, n = 15) were not treated.Then, The guinea pigs in group A and B were exposed in noise for 8 days, 8 hours once a day.Auditory brainstem response (ABR) was used to test the hearing threshold of guinea pigs thrice, first before the drug administered, then after 6 weeks the drug used, third after noise exposure.And colorimetry was used to analyze the activity of superoxide dismutase (SOD) and malon dialdehyde (MDA) in brain and liver tissue.The DNA of inner ear tissue was harvested and amplified fragment length polymorphism (AFLP) was used to detect the differential polymorphic markers.Results After injection, there was no significant difference in elevation of ABR threshold between the group A and group B ( t = 1.14, P>0.05 ).However, exposure of noise later, elevation in ABR threshold of ( 22.97±10.56) dB PeSPL was observed in group A, and ( 14.16±7.36) dB peSPL in group B.The was significant difference in variation of hearing threshold between group A and group B ( t = 2.78 ,P<0.05 ).The activity of SOD in brain and liver tissue in group A was lower than that in group B.the level of MDA was opposite between group A and group B.The difference between group A and group B was significant ( P<0.01 ) .A differential polymorphic marker was observed by AFLP. Conclusions The mimetic aging effect of the guinea pigs can be induced by D-galactose, and this model can not directly induce the hearing loss.The differential polymorphic marker possibly act as a predisposing factor which can greatly enhance the sensitivity of the ear to the noise.
