中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
6期
411-414
,共4页
西兰%张小婷%赵莹%蔡泽明%张斌%巩毓刚%章星琪
西蘭%張小婷%趙瑩%蔡澤明%張斌%鞏毓剛%章星琪
서란%장소정%조형%채택명%장빈%공육강%장성기
斑秃%丝聚蛋白%免疫
斑禿%絲聚蛋白%免疫
반독%사취단백%면역
Alopecia areata%Filaggrin%Immunity
目的 探讨丝聚蛋白表达水平与斑秃患者特应性素质和疾病严重程度的关系.方法 分析37例斑秃患者的临床资料及实验室资料,取斑秃皮损和正常对照的头皮进行免疫组化染色,用荧光半定量RT-PCR检测22例斑秃皮损和正常对照的头皮丝聚蛋白在蛋白质和信使RNA的表达水平.结果 斑秃皮损丝聚蛋白和其mRNA的表达水平较正常对照明显降低(P< 0.05或0.01),而且这种降低在脱发面积较大、病程较长和有指甲改变的斑秃患者中更明显,但降低水平与是否伴发特应性疾病无关.斑秃患者伴特应性疾病组与不伴特应性疾病组之间性别、发病年龄、病程、脱发面积、家族史、甲改变、血清IgE和嗜酸粒细胞升高的发生率等方面差异均无统计学意义.结论 斑秃患者其皮损丝聚蛋白及其mRNA的表达水平均降低,提示丝聚蛋白可能参与斑秃的发病,并和疾病的严重程度有关.
目的 探討絲聚蛋白錶達水平與斑禿患者特應性素質和疾病嚴重程度的關繫.方法 分析37例斑禿患者的臨床資料及實驗室資料,取斑禿皮損和正常對照的頭皮進行免疫組化染色,用熒光半定量RT-PCR檢測22例斑禿皮損和正常對照的頭皮絲聚蛋白在蛋白質和信使RNA的錶達水平.結果 斑禿皮損絲聚蛋白和其mRNA的錶達水平較正常對照明顯降低(P< 0.05或0.01),而且這種降低在脫髮麵積較大、病程較長和有指甲改變的斑禿患者中更明顯,但降低水平與是否伴髮特應性疾病無關.斑禿患者伴特應性疾病組與不伴特應性疾病組之間性彆、髮病年齡、病程、脫髮麵積、傢族史、甲改變、血清IgE和嗜痠粒細胞升高的髮生率等方麵差異均無統計學意義.結論 斑禿患者其皮損絲聚蛋白及其mRNA的錶達水平均降低,提示絲聚蛋白可能參與斑禿的髮病,併和疾病的嚴重程度有關.
목적 탐토사취단백표체수평여반독환자특응성소질화질병엄중정도적관계.방법 분석37례반독환자적림상자료급실험실자료,취반독피손화정상대조적두피진행면역조화염색,용형광반정량RT-PCR검측22례반독피손화정상대조적두피사취단백재단백질화신사RNA적표체수평.결과 반독피손사취단백화기mRNA적표체수평교정상대조명현강저(P< 0.05혹0.01),이차저충강저재탈발면적교대、병정교장화유지갑개변적반독환자중경명현,단강저수평여시부반발특응성질병무관.반독환자반특응성질병조여불반특응성질병조지간성별、발병년령、병정、탈발면적、가족사、갑개변、혈청IgE화기산립세포승고적발생솔등방면차이균무통계학의의.결론 반독환자기피손사취단백급기mRNA적표체수평균강저,제시사취단백가능삼여반독적발병,병화질병적엄중정도유관.
Objective To assess the relationship of filaggrin expression with atopic diathesis and disease severity in patients with alopecia areata (AA).Methods Thirty-seven patients with AA aged (26.3 ± 10.6) years were enrolled in this study.Atopic diseases were noted in 8 of these patients.Clinical data and laboratory test resuhs were reviewed.Immunohistochemical staining was performed to quantify the expression of filaggrin protein in scalp biopsy specimens from all of the 37 patients with AA and from 10 human controls,and fluorescence-based semiquantitative reverse transcription-PCR to detect the expression of filaggrin mRNA in scalp biopsy specimens from 22 patients with AA and 13 healthy controls.Data were statistically analyzed by Mann Whitney U test,chi-square test,and Spearman's rank correlation test.Results The expressions of filaggrin protein and mRNA were significantly lower in patients with AA than in the controls (P < 0.05 or 0.01 ),and the decrease seemed more obvious in patients with large areas of lesions,long duration of disease,and nail abnormalities,but the degree of decrease was unrelated to the complication with atopic diseases.No significant differences were observed in sex ratio,age at onset,disease duration,area of hair loss,the prevalence of family history or incidence of nail abnormalities and increase in serum IgE and eosinophils,between patients with atopic diseases and those without.Conclusions The expressions of filaggrin protein and mRNA are decreased in patients with AA,suggesting that filaggrin may participate in the development of AA and is correlated with the severity of AA.