中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
6期
426-428
,共3页
吴蓓玲%孙洁%杨晓红%汤国根%刘改荣%陈琼%王虹%程立峰%戴文静
吳蓓玲%孫潔%楊曉紅%湯國根%劉改榮%陳瓊%王虹%程立峰%戴文靜
오배령%손길%양효홍%탕국근%류개영%진경%왕홍%정립봉%대문정
白癜风%卡介菌多糖核酸%中草药%T-淋巴细胞亚群
白癜風%卡介菌多糖覈痠%中草藥%T-淋巴細胞亞群
백전풍%잡개균다당핵산%중초약%T-림파세포아군
Vitiligo%BCG-PSN%Drugs,Chinese herbal%T-Lymphocyte subsets
目的 探讨卡介菌多糖核酸(BCG-PsN)联合中药白癜风颗粒治疗白癜风临床疗效及对免疫的调节作用.方法 流式细胞仪和ELISA法检测BCG-PSN联合白癜风颗粒治疗白癜风前后T细胞亚群及外周血淋巴细胞Fas及FasL的表达水平,并与BCG-PSN及白癜风颗粒单一治疗对照组进行比较.结果 BCG-PSN联合白癜风颗粒治疗组总有效率达82.86%,明显高于BCG-PSN对照组的40.63%(P<0.01).各组治疗前外周血CD3+、CD3+CD4+、CD3+CD8+均低于健康对照组(P<0.01),CD4+/CD8+比值升高(P<0.01),Fas表达明显降低(P<0.01).治疗后CD3+、CD3+CD4+、CDTCD8+治疗前升高(P<0.05),CD4+/CD8+比值下降(P<0.05),Fas明显上升(P<0.01).结论 BCG-PSN可通过逆转外周血淋巴细胞的Fas/FasL表达异常,诱导淋巴细胞的正常凋亡,与中药合用起协同作用.
目的 探討卡介菌多糖覈痠(BCG-PsN)聯閤中藥白癜風顆粒治療白癜風臨床療效及對免疫的調節作用.方法 流式細胞儀和ELISA法檢測BCG-PSN聯閤白癜風顆粒治療白癜風前後T細胞亞群及外週血淋巴細胞Fas及FasL的錶達水平,併與BCG-PSN及白癜風顆粒單一治療對照組進行比較.結果 BCG-PSN聯閤白癜風顆粒治療組總有效率達82.86%,明顯高于BCG-PSN對照組的40.63%(P<0.01).各組治療前外週血CD3+、CD3+CD4+、CD3+CD8+均低于健康對照組(P<0.01),CD4+/CD8+比值升高(P<0.01),Fas錶達明顯降低(P<0.01).治療後CD3+、CD3+CD4+、CDTCD8+治療前升高(P<0.05),CD4+/CD8+比值下降(P<0.05),Fas明顯上升(P<0.01).結論 BCG-PSN可通過逆轉外週血淋巴細胞的Fas/FasL錶達異常,誘導淋巴細胞的正常凋亡,與中藥閤用起協同作用.
목적 탐토잡개균다당핵산(BCG-PsN)연합중약백전풍과립치료백전풍림상료효급대면역적조절작용.방법 류식세포의화ELISA법검측BCG-PSN연합백전풍과립치료백전풍전후T세포아군급외주혈림파세포Fas급FasL적표체수평,병여BCG-PSN급백전풍과립단일치료대조조진행비교.결과 BCG-PSN연합백전풍과립치료조총유효솔체82.86%,명현고우BCG-PSN대조조적40.63%(P<0.01).각조치료전외주혈CD3+、CD3+CD4+、CD3+CD8+균저우건강대조조(P<0.01),CD4+/CD8+비치승고(P<0.01),Fas표체명현강저(P<0.01).치료후CD3+、CD3+CD4+、CDTCD8+치료전승고(P<0.05),CD4+/CD8+비치하강(P<0.05),Fas명현상승(P<0.01).결론 BCG-PSN가통과역전외주혈림파세포적Fas/FasL표체이상,유도림파세포적정상조망,여중약합용기협동작용.
Objective To estimate the clinical efficacy and immunomodulatory effect of polysaccharide nucleic acid fraction of bacillus calmette guerin (BCG-PSN) combined with a traditional Chinese medicine in patients with vitiligo.Methods This study recruited 99 patients with vitiligo aged from 13 to 65 (35,6 ± 5.8) years.The patients were classified into 3 groups to be treated with BCG-PSN and a traditional Chinese medicine (Baidianfeng granules) alone or in combination.BCG-PSN was intramuscularly injected at a dose of 2 ml every other day and baidianfeng granules were given orally thrice a day,for 3 months.Peripheral blood samples were obtained from the patients at the baseline and after the end of treatment and from 30 healthy controls.Flow cytometry and enzyme linked immunosorbent assay (ELISA) were performed to detect T cell subsets and expression level of Fas and Fas ligand (FasL),respectively.Data were analyzed by t test and chi-square test.Results The response rate was significantly higher in patients treated with BCG-PSN combined with Baidianfeng granules than in those with BCG-PSN alone (82.86% vs.40.63%,P < 0.01 ).Before the treatment,patients showed a lower percentage of CD3+ cells,CD3+CD4+ cells and CD3+CD8+ cells in peripheral blood (all P <0.01 ),weaker expression of Fas (P < 0.01 ),but a higher CD4/CD8 ratio (P < 0.01 ) compared with the controls.The treatment with BCG-PSN and Baidianfeng granules alone or in combinatiou all induced an increase in the percentage of CD3+ cells,CD3+CD4+ cells and CD3+CD8+ cells in peripheral blood (P < 0.05) and in the expression of Fas (P < 0.01),but a decrease in CD4/CD8 ratio (P < 0.05).Conclusions BCG-PSN may induce the normal apoptosis in lymphocytes via reversing the abnormality in the expression of Fas/FasL by peripheral blood lymphocytes,and the effect of BCG-PSN may be enhanced by a traditional Chinese medicine,Baidianfeng granules.