CAJ | 학술논문

目的利用基因芯片技术研究肾性高血压大鼠脑梗死后期梗死灶边缘区皮层基因表达谱的改变及其意义.方法利用双肾双夹法复制肾性高血压大鼠模型,再采用线栓法复制成大脑中动脉闭塞模型,并设置假手术组,术后7 d取梗死灶边缘区域脑皮层,提取RNA,经过荧光标记后与含5705个基因的oligo芯片进行杂交、扫描,采集图像,经数据分析筛选出差异表达的基因.结果差异表达基因共197个(包括表达序列标签12个),其中表达上调174个,下调23个.12类功能分组的基因均有上调,下调的基因仅包括运输、转录调控、信号、应激反应、代谢和细胞粘附组的基因.12类功能分组中有17个差异表达基因尚未有文献报道与脑缺血/梗死相关.结论脑梗死后期基因表达仍然非常活跃,预示着损伤与修复的分子机制和可能的治疗靶点.
목적 이용기인심편기술연구신성고혈압대서뇌경사후기경사조변연구피층기인표체보적개변급기의의.방법 이용쌍신쌍협법복제신성고혈압대서모형,재채용선전법복제성대뇌중동맥폐새모형,병설치가수술조,술후7 d취경사조변연구역뇌피층,제취RNA,경과형광표기후여함5705개기인적oligo심편진행잡교、소묘,채집도상,경수거분석사선출차이표체적기인.결과 차이표체기인공197개(포괄표체서렬표첨12개),기중표체상조174개,하조23개.12류공능분조적기인균유상조,하조적기인부포괄운수、전록조공、신호、응격반응、대사화세포점부조적기인.12류공능분조중유17개차이표체기인상미유문헌보도여뇌결혈/경사상관.결론 뇌경사후기기인표체잉연비상활약,예시착손상여수복적분자궤제화가능적치료파점.
Objective To analyze the gene expression profiling in the periinfarct cortex in the late stage after stroke onset in renovascular hypertensive rats (RHRs) with gene chip technology.Methods RHRs were induced by the method of Goldblatt's 2k2c operation. Cerebral infarction was also induced by permanent middle cerebral artery occlusion (pMCAO) in RHRs. Sham-operated group was established as controls. Total RNA was extracted from the perinfarct cerebral cortex in injured hemisphere 7 d after pMCAO, and the RNA was performed fluorescence labeling, followed by hybridization with 5705 oligo chips. And then, scanning was performed; the data was collected and chosen for microarray analysis using oligonucleotide arrays. Resuits In total, 197 genes were expressed differentially, including 174 genes up-regulated expression and 23 genes down-regulated expression. The up-regulated genes were distributed among all 12 functional categories; the down-regulated genes were distributed only in the categories of transport, transcription regulator, signal,response to stress, metabolism and cell adhesion. Among the 12 functional categories, only 17differentially expressed genes were not previously reported to be associated with brain ischemia/infarction. Conelusion Active gene expression at late stage of cerebral infarction may imply the molecular mechanisms of injury or repair, being the targets of therapeutic intervention.