CAJ | 학술논문

目的观察表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒(EPI-PBCA-MNPS)对裸鼠移植性肝癌的作用效果.方法建立Bel-7402裸鼠人肝癌模型,当肿瘤体积长至20～30mm3时,将其随机分成5组:生理盐水组(NS组)、表阿霉素组(EPI组)、表阿霉素聚氰基丙烯酸正丁酯纳米粒组(EPI-PBCA-NPS组)、表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒组(EPI-PBCA-MNPS组)、表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒组加磁场组(EPI-PBCA-MNPS+MF组),每组10只,除生理盐水组外,其余各组按照0.002mg/g体质量或相当于40 μg/只的表阿霉素静脉注射给药,治疗2周后处死裸鼠,准确测量肿瘤大小并称重,计算瘤体积抑制率及瘤重抑制率,所有肿瘤均编号病理切片,比较治疗前后各组肿瘤细胞坏死的程度.结果治疗后肿瘤体积抑制率、瘤重抑制率除EPI-PBCA-NPS组与EPI-PBCA-MNPS组差异无统计学意义(P>0.05)外,其余各组间差异有统计学意义(P<0.05),其中EPI-PBCA-MNP+MF组(94.26%、80.82%)显著高于其余组,差异有统计学意义(P<0.01).病理切片显示,EPI-PBCA-MNP+MF组肿瘤细胞坏死程度最重.结论 EPI-PBCA-MNPS具有良好的靶向性,在外加磁场作用下对裸鼠人肝癌模型有显著抑瘤作用.
목적 관찰표아매소취청기병희산정정지자성납미립(EPI-PBCA-MNPS)대라서이식성간암적작용효과.방법 건립Bel-7402라서인간암모형,당종류체적장지20～30mm3시,장기수궤분성5조:생리염수조(NS조)、표아매소조(EPI조)、표아매소취청기병희산정정지납미립조(EPI-PBCA-NPS조)、표아매소취청기병희산정정지자성납미립조(EPI-PBCA-MNPS조)、표아매소취청기병희산정정지자성납미립조가자장조(EPI-PBCA-MNPS+MF조),매조10지,제생리염수조외,기여각조안조0.002mg/g체질량혹상당우40 μg/지적표아매소정맥주사급약,치료2주후처사라서,준학측량종류대소병칭중,계산류체적억제솔급류중억제솔,소유종류균편호병리절편,비교치료전후각조종류세포배사적정도.결과 치료후종류체적억제솔、류중억제솔제EPI-PBCA-NPS조여EPI-PBCA-MNPS조차이무통계학의의(P>0.05)외,기여각조간차이유통계학의의(P<0.05),기중EPI-PBCA-MNP+MF조(94.26%、80.82%)현저고우기여조,차이유통계학의의(P<0.01).병리절편현시,EPI-PBCA-MNP+MF조종류세포배사정도최중.결론 EPI-PBCA-MNPS구유량호적파향성,재외가자장작용하대라서인간암모형유현저억류작용.
Objective To observe the effect of epirubicin polybutylcyanoacrylate magnetic nanoparticles (EPI-PBCA-MNPS) on the transplanted hepatoma in nude mice.Methods Human hepatoma cell line Bel-7402 nude mice models were established and divided into 5 groups with each of 10 once gross tumor volume was up to 20-30 mm3:normal saline group (NS),epirubicin group (EPI),epirubicin polybutylcyanoacrylate nanoparticles group (EPI-PBCA-NPS),epirubicin polybutylcyanoacrylate magnetic nanoparticles group (EPI-PBCA-MNPS) and epirubicin polybutylcyanoacrylate magnetic nanoparticles+magnetic field group(EPI-PBCA-MNPS+MF).Except for NS group, the dosage of epirubicin was 0.002 mg/g body weight or 40 μg for intravenous injection.After two-weeks therapy,all nude mice models were executed and the tumor volume and weight were measured so as to calculate the volume inhibition ratio and the weight inhibition ratio.Every tumor specimen was made into pathological section and labelled with serial number for comparing neorobiosis degree in different groups before and after therapy.Results There were statistically difference among the other groups (P<0.05) in the volume inhibition ratio and the weight inhibition ratio after therapy except for EPI-PBCA-NPS and EPI-PBCA-MNPS group (P>0.05).The two ratios in the EPI-PBCA-MNP+MF group (94.26%,80.82%) were significantly more than those in other groups (P<0.01).The pathological sections demonstrated the most severe tumour neorobiosis in the EPI-PBCA-MNP+MF group.Conclusion With the additional magnetic field,EPI-PBCA-MNPS has significant anticancer effect on human hepatoma nude mice models due to its favourable magnetic targeting.