CAJ | 학술논문

目的探讨超顺磁性氧化铁颗粒(SPIO)对于骨髓间充质干细胞(BMSCs)的标记能力以及合适的标记条件.方法采集成年犬的BMSCs,进行体外培养扩增后,行SPIO标记,观察不同条件下BMSCs的SPIO标记情况及SPIO对BMSCs活性的影响.结果 4.5μg/ml及9.0μg/ml标记组细胞内含有大量的SPIO颗粒,细胞活性未受影响.细胞内铁颗粒的数量随着标记物浓度的增加而增加.18.0μg/ml标记组细胞内SPIO聚集成团,部分细胞崩解.标记了SPIO的BMSCs达到一定细胞数量(5.0×104)时,可以在体外磁共振显像(MRI).随着标记细胞的培养时间延长,细胞内SPIO颗粒逐渐减少(标记1、2、4、8周BMCSs台盼蓝拒染率分别为92.04±1.02、91.08±1.09、90.03±2.03和89.93±1.02),细胞死亡率变化没有统计学意义(P＞0.05).结论 SPIO可以作为一种很好的细胞示踪剂应用于体外细胞示踪.
목적 탐토초순자성양화철과립(SPIO)대우골수간충질간세포(BMSCs)적표기능력이급합괄적표기조건.방법 채집성년견적BMSCs,진행체외배양확증후,행SPIO표기,관찰불동조건하BMSCs적SPIO표기정황급SPIO대BMSCs활성적영향.결과 4.5μg/ml급9.0μg/ml표기조세포내함유대량적SPIO과립,세포활성미수영향.세포내철과립적수량수착표기물농도적증가이증가.18.0μg/ml표기조세포내SPIO취집성단,부분세포붕해.표기료SPIO적BMSCs체도일정세포수량(5.0×104)시,가이재체외자공진현상(MRI).수착표기세포적배양시간연장,세포내SPIO과립축점감소(표기1、2、4、8주BMCSs태반람거염솔분별위92.04±1.02、91.08±1.09、90.03±2.03화89.93±1.02),세포사망솔변화몰유통계학의의(P＞0.05).결론 SPIO가이작위일충흔호적세포시종제응용우체외세포시종.
Objective To explore labeling efficiency and appropriate conditions of Superpara magnetic iron exide nanopaticles (SPIO) nanoparticles for Bone marrow stromal cells(BMSCs). Methods BMSCs were aquired from skeletally mature dogs via iliac crest aspiration and separated by adherent cell cytopheresis.BMSCs were cultured and incubated with SPIO at different concentrations in vitro. The labeling efficiency of BMSCs with different labeling concentrations SPIO nanoparticles as well as detection of characteristics and signal attenuation rules were evaluated by MRI at 1.5T in vitro. Results BMSCs were efficiently labeled by SPIOin vitro and has no alterations to viability and proliferation profiles at this labeling concentration. BMSCs loaded with SPIO can be detected by MRI at certainly cell quantity in vitro(5 × 104). The quantity of SPIO in cells gradually reduced as cell culture time prolonged, with no statistically significant changes in cell death(P＞ 0.05). Conclusion The results demonstrated the potential application of SPIO as a wonderful cell tracer in vitro.