中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
6期
560-563
,共4页
朱小霞%程浩%张行%朱可建%周强%陈大方%王琦
硃小霞%程浩%張行%硃可建%週彊%陳大方%王琦
주소하%정호%장행%주가건%주강%진대방%왕기
荧光定量PCR%尖锐湿疣%人乳头瘤病毒%Toll样受体
熒光定量PCR%尖銳濕疣%人乳頭瘤病毒%Toll樣受體
형광정량PCR%첨예습우%인유두류병독%Toll양수체
Quantitative fluorescent PCR%Condyloma acuminatum%HPV%TLR
目的 检测尖锐湿疣皮损中Toll样受体(TLRs)的mRNA表达水平,探讨TLRs在人乳头瘤病毒(HPV)感染免疫中的可能作用.方法 提取40例尖锐湿疣患者皮损和35例HPV阴性的慢性宫颈炎患者宫颈刮落细胞的总RNA,逆转录为cDNA后用实时荧光定量PCR法检测TLR1～10的mRNA表达水平,并检测40例尖锐湿疣的HPV分型.结果 40例尖锐湿疣患者以低危型HPV6和11型为主(77.5%和55%).55%尖锐湿疣患者感染两种以上HPV亚型,其中35%合并高危型HPV感染.尖锐湿疣组TLR3、7、8的mRNA表达水平高于其他TLRs,TLR9的mRNA表达水平低于其他TLRs(P均<0.05).尖锐湿疣皮损TLR1～10的mRNA表达水平在HPV6型和HPV11型感染间以及在低危型与合并高危型HPV感染间差异无统计学意义(P均>0.05).尖锐湿疣组TLR1～3、TLR5～8和TLR10的mRNA表达水平均高于HPV阴性的慢性宫颈炎组(P均<0.05),其中TLR2、7、8的mRNA表达水平升高更显著(P均<0.01).而TLR4、TLR9的mRNA表达水平与对照组差异无统计学意义(P>0.05).结论 尖锐湿疣皮损中部分TLRs(3、7、8)表达水平较高,但TLR9表达水平较低.相对于HPV阴性的慢性宫颈炎,尖锐湿疣皮损中TLR1~3、TLR5～8和TLR10的mRNA表达上调.不同HPV型别感染的尖锐湿疣皮损中TLRs表达谱差异无统计学意义.推测尖锐湿疣TLRs表达谱的改变可能与HPV感染有一定关系,是否与HPV感染的免疫逃逸机制及持续感染有关尚有待进一步明确.
目的 檢測尖銳濕疣皮損中Toll樣受體(TLRs)的mRNA錶達水平,探討TLRs在人乳頭瘤病毒(HPV)感染免疫中的可能作用.方法 提取40例尖銳濕疣患者皮損和35例HPV陰性的慢性宮頸炎患者宮頸颳落細胞的總RNA,逆轉錄為cDNA後用實時熒光定量PCR法檢測TLR1～10的mRNA錶達水平,併檢測40例尖銳濕疣的HPV分型.結果 40例尖銳濕疣患者以低危型HPV6和11型為主(77.5%和55%).55%尖銳濕疣患者感染兩種以上HPV亞型,其中35%閤併高危型HPV感染.尖銳濕疣組TLR3、7、8的mRNA錶達水平高于其他TLRs,TLR9的mRNA錶達水平低于其他TLRs(P均<0.05).尖銳濕疣皮損TLR1～10的mRNA錶達水平在HPV6型和HPV11型感染間以及在低危型與閤併高危型HPV感染間差異無統計學意義(P均>0.05).尖銳濕疣組TLR1～3、TLR5～8和TLR10的mRNA錶達水平均高于HPV陰性的慢性宮頸炎組(P均<0.05),其中TLR2、7、8的mRNA錶達水平升高更顯著(P均<0.01).而TLR4、TLR9的mRNA錶達水平與對照組差異無統計學意義(P>0.05).結論 尖銳濕疣皮損中部分TLRs(3、7、8)錶達水平較高,但TLR9錶達水平較低.相對于HPV陰性的慢性宮頸炎,尖銳濕疣皮損中TLR1~3、TLR5～8和TLR10的mRNA錶達上調.不同HPV型彆感染的尖銳濕疣皮損中TLRs錶達譜差異無統計學意義.推測尖銳濕疣TLRs錶達譜的改變可能與HPV感染有一定關繫,是否與HPV感染的免疫逃逸機製及持續感染有關尚有待進一步明確.
목적 검측첨예습우피손중Toll양수체(TLRs)적mRNA표체수평,탐토TLRs재인유두류병독(HPV)감염면역중적가능작용.방법 제취40례첨예습우환자피손화35례HPV음성적만성궁경염환자궁경괄락세포적총RNA,역전록위cDNA후용실시형광정량PCR법검측TLR1～10적mRNA표체수평,병검측40례첨예습우적HPV분형.결과 40례첨예습우환자이저위형HPV6화11형위주(77.5%화55%).55%첨예습우환자감염량충이상HPV아형,기중35%합병고위형HPV감염.첨예습우조TLR3、7、8적mRNA표체수평고우기타TLRs,TLR9적mRNA표체수평저우기타TLRs(P균<0.05).첨예습우피손TLR1～10적mRNA표체수평재HPV6형화HPV11형감염간이급재저위형여합병고위형HPV감염간차이무통계학의의(P균>0.05).첨예습우조TLR1～3、TLR5～8화TLR10적mRNA표체수평균고우HPV음성적만성궁경염조(P균<0.05),기중TLR2、7、8적mRNA표체수평승고경현저(P균<0.01).이TLR4、TLR9적mRNA표체수평여대조조차이무통계학의의(P>0.05).결론 첨예습우피손중부분TLRs(3、7、8)표체수평교고,단TLR9표체수평교저.상대우HPV음성적만성궁경염,첨예습우피손중TLR1~3、TLR5～8화TLR10적mRNA표체상조.불동HPV형별감염적첨예습우피손중TLRs표체보차이무통계학의의.추측첨예습우TLRs표체보적개변가능여HPV감염유일정관계,시부여HPV감염적면역도일궤제급지속감염유관상유대진일보명학.
Objective To investigate the expression of Toll-like receptors(TLRs) in condyloma acuminatum(CA) lesions and their possible roles in the pathogenesis of CA. Methods The expressions of TLR1-10 mRNA level in the lesions of CA and in the cervix scrape cells from the patients with human papillomavirus(HPV) negative chronic cervicitis were detected by real-time quantitative fluorescent PCR. HPV typing was detected by HPV GenoArray test kit. Results Low-risk HPV type 6 and type 11 were the most prevalent types in the forty CA cases with positive rate of 77.5% and 55% respectively. 55% CA patients were found infected with more than two types of HPV. 35% CA patients were concurrently infected with high-risk HPV. The expressions of TLR3, 7, 8 mRNA were higher than other TLRs and the expression of TLR9 mRNA was lower than others in the lesions of CA. No significant differences of the TLR1-10 mRNA levels were found between HPV6 and HPV11 positive CA lesions, so did it between low-risk and high-risk HPV concurrent infected CA lesions. The expressions of TLR1-3, TLR5-8, TLR10 mRNA, especially TLR2, TLR7 and TLR8 in the lesions of CA were significantly higher than that in cervix scrape cells of HPV negative chronic cervicitis. There were no significant differences of TLR4 and TLR9 mRNA levels between the two groups. Conclusion There were higher expressions of some TLRs (3, 7, 8) and lower expression of TLR9 in the lesions of CA. Compared with HPV negative chronic cervicitis, the expressions of TLR1-3, TLR5-8, TLR10 mRNA in the lesions of CA were up-regulated. The expression profile of TLRs in different type of HPV infected CA lesions had no significant differences. Our results suggested that the expression profile of TLRs in CA may be associated with the HPV infection. Whether it was associated with the immune escape mechanism and persistent infection of HPV need further demonstration.
