CAJ | 학술논문

目的:探讨在缺氧性损害下肼屈嗪对视网膜色素上皮(ARPE-19)细胞的抗氧化效果以及活性氧(ROS)在此效果中的作用.方法:用人视网膜色素上皮细胞研究肼屈嗪对氧化应激的作用,包括特丁基氢过氧化物(t-BHP)、过氧化氢(H2O2)、叠氮化钠(NaN3),以及缺氧引起的细胞坏死.用MTT检验测试细胞活性.结果:用ROS诱导的氧化应激治疗ARPE-19细胞,肼屈嗪在抵抗t-BHP、H2O2、缺氧引起的细胞坏死中表现出浓度依赖性,但对NaN3不具有浓度依赖性.这一作用中不涉及到一氧化氮(NO).结论:肼屈嗪在ARPE-19细胞中表现出抗氧化应激诱导的破坏,这一作用可能是因为对ROS的净化剂作用.所以肼屈嗪可能用于治疗老年黄斑变性.
목적:탐토재결양성손해하정굴진대시망막색소상피(ARPE-19)세포적항양화효과이급활성양(ROS)재차효과중적작용.방법:용인시망막색소상피세포연구정굴진대양화응격적작용,포괄특정기경과양화물(t-BHP)、과양화경(H2O2)、첩담화납(NaN3),이급결양인기적세포배사.용MTT검험측시세포활성.결과:용ROS유도적양화응격치료ARPE-19세포,정굴진재저항t-BHP、H2O2、결양인기적세포배사중표현출농도의뢰성,단대NaN3불구유농도의뢰성.저일작용중불섭급도일양화담(NO).결론:정굴진재ARPE-19세포중표현출항양화응격유도적파배,저일작용가능시인위대ROS적정화제작용.소이정굴진가능용우치료노년황반변성.
AIM: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect.METHODS: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay.RESULTS: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect.CONCLUSION: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).