CAJ | 학술논문

目的探测儿茶酚氧位甲基转移酶(COMT)基因、载脂蛋白E(Apo-E)基因及五羟色胺受体2A(5-HT2a)基因多态性与情感障碍之关系,搜寻中国汉族人情感障碍易患性基因.方法应用聚合酶链反应(PCR)和限制性片段长度多态性(RFLP)方法对5个情感障碍高发家系进行初步研究.结果用改进的传递/不平衡(TDT)检测结果表明,在5个情感障碍高发家系中,COMT基因与情感障碍相关联(P＜0.05),而Apo-E基因和5-HT2a基因不与情感障碍关联(P＞0.05).结论在我们研究的家系中,22号染色体和长臂(22q11.2)可能存在情感障碍易患性基因.
목적탐측인다분양위갑기전이매(COMT)기인、재지단백E(Apo-E)기인급오간색알수체2A(5-HT2a)기인다태성여정감장애지관계,수심중국한족인정감장애역환성기인.방법응용취합매련반응(PCR)화한제성편단장도다태성(RFLP)방법대5개정감장애고발가계진행초보연구.결과용개진적전체/불평형(TDT)검측결과표명,재5개정감장애고발가계중,COMT기인여정감장애상관련(P＜0.05),이Apo-E기인화5-HT2a기인불여정감장애관련(P＞0.05).결론재아문연구적가계중,22호염색체화장비(22q11.2)가능존재정감장애역환성기인.
Objective To explore the relationship between polymorphism of COMT gene and Apo-E gene and 5-HT2a gene with affective disorder. Search for a gene predisposing to affective disorder in the Han nationality of Chinese. Methods Five pedigrees with high incidence of affective disorder were preliminary studied by polymerize chain reaction(PCR) and restriction fragment length polymorphism(RFLP) technique. Results Analysis results of statistics of the transmission/disequilibrium test (TDT) handing showed that the COMT gene is association with affective disorder (P<0.05),the Apo-E gene and the 5-HT2a gene are not association for affective disorder(P>0.05) in the five pedigree with high incidence affective disorder.Conclusion Our study results suggest that maybe a affective disorder liability gene on 22chromosome (22q11.2) in our studied pedigrees.