CAJ | 학술논문

建立了一种快速、有效分离测定茜草科植物匙鄂木中3种蒽醌类化合物的微乳液毛细管电动色谱新方法.研究了硼砂浓度、微乳液浓度、乙腈浓度以及电压对分离行为的影响.实验结果表明:最佳缓冲体系组成为10 mmol/L硼砂-12%微乳储备液-5%乙腈,最佳运行电压为25 kv,检测波长为264nm.分析物浓度与峰面积之间的线性关系良好,相关系数在0.9992～0.9995之间.该方法用于对匙鄂木中蒽醌类化合物的含量测定,回收率为97.3%～106.2%.
건립료일충쾌속、유효분리측정천초과식물시악목중3충은곤류화합물적미유액모세관전동색보신방법.연구료붕사농도、미유액농도、을정농도이급전압대분리행위적영향.실험결과표명:최가완충체계조성위10 mmol/L붕사-12%미유저비액-5%을정,최가운행전압위25 kv,검측파장위264nm.분석물농도여봉면적지간적선성관계량호,상관계수재0.9992～0.9995지간.해방법용우대시악목중은곤류화합물적함량측정,회수솔위97.3%～106.2%.
A rapid and effective microemulsion electrokinetic chromatography method was established for simultaneous determination of three bioactive components (anthraquinones) extract of Chinese herb Paedicalyx attopevensis Pierre ex Pitard for the first time.After optimization of separation conditions,baseline separation was obtained for the three analytes within 8 rain using a running buffer composed of 10 mmol/L borate solution+12% microemulsion stock solution [sodium dodecyl sulphate (SDS)+lbutanol+n-heptane+water=16.55%+33.05%+4.05%+46.35% (w/w)]+5% acetonitrile.Regression equations revealed linear relationships (correlation coefficients 0.999 2～0.999 5) between the peak area of each analyte and the concentration.The relative standard deviations of the migration times and the peak areas of the three constituents were less than 0.53% and 3.2%.The recoveries of three constituents ranged from 97.3% to 106.2%.