CAJ | 학술논문

目的:观察瘦素对体外培养兔关节软骨细胞分泌一氧化氮(NO)和基质金属蛋白酶-13(MMP-13)的影响.方法:获取2月龄兔原代软骨细胞,培养、鉴定、传代.将第3代软骨细胞按实验要求种板培养,饥饿12 h后,以不同浓度瘦素单独或与 TNF-α联合干预48或96 h,测定各组细胞上清液中NO及MMP-13的浓度.结果:不同浓度瘦素(5,10,15和20 μg/mL)组NO浓度与空白对照组差异无统计学意义(P＞0.05);而不同浓度的瘦素与TNF-α(10 ng/mL)联合应用后,与TNF-α组差异有统计学意义(P＜0.05).空白对照组并未测出MMP-13的存在,不同瘦素浓度(10,50和100 ng/mL)组测得MMP-13浓度在剂量主效应和时间主效应均有统计学意义(P＜0.05).结论:瘦素在体外能协同TNF-α促进兔关节软骨细胞分泌NO和MMP-13.
목적:관찰수소대체외배양토관절연골세포분비일양화담(NO)화기질금속단백매-13(MMP-13)적영향.방법:획취2월령토원대연골세포,배양、감정、전대.장제3대연골세포안실험요구충판배양,기아12 h후,이불동농도수소단독혹여 TNF-α연합간예48혹96 h,측정각조세포상청액중NO급MMP-13적농도.결과:불동농도수소(5,10,15화20 μg/mL)조NO농도여공백대조조차이무통계학의의(P＞0.05);이불동농도적수소여TNF-α(10 ng/mL)연합응용후,여TNF-α조차이유통계학의의(P＜0.05).공백대조조병미측출MMP-13적존재,불동수소농도(10,50화100 ng/mL)조측득MMP-13농도재제량주효응화시간주효응균유통계학의의(P＜0.05).결론:수소재체외능협동TNF-α촉진토관절연골세포분비NO화MMP-13.
Objective To observe the in vitro effect of leptin, alone or in combination with tumor necrosis factor-alpha (TNF-α) on inducible nitric oxide (NO) and on inducible matrix metallo-proteinase-1 3 (MMP-13) in rabbit articular chondrocytes. Methods The chondrocytes from the articular cartilage of 2-month-old rabbits were cultivated and identified, and the second filial generation chondrocytes were cocultured on plates with different concentrations of leptin alone or in combination with TNF-α for 48 h or 96 h after 12 h starvation. The concentration of NO and MMP-13 was measured in the chondrocytes culture supernatant fluid. The results were statistically analyzed. Results There was no significant difference in the concentrations of NO between the different concentrations of leptin alone groups and the blank control group (P ＞ 0. 05). In combination with the same concentration of TNF-α (10 ng/mL), leptin could dose-dependently increase the concentration of NO in the chondrocytes culture supernatant fluid in vitro. There was significant value in average concentration of MMP-13 on the main effect of both time and dose (P ＜0. 05) . No MMP-13 was detected in the blank control group. Conclusion Leptin can induce MMP-13 and have synergistic induction effect on NO with TNF-α in rabbit articular chondrocytes in vitro.