上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2010年
2期
200-203,207
,共5页
牛陵川%邹文兵%张炼%朱辉%向理科%王智彪
牛陵川%鄒文兵%張煉%硃輝%嚮理科%王智彪
우릉천%추문병%장련%주휘%향이과%왕지표
高强度聚焦超声%肿瘤%消融%增殖细胞核抗原%乳腺癌
高彊度聚焦超聲%腫瘤%消融%增殖細胞覈抗原%乳腺癌
고강도취초초성%종류%소융%증식세포핵항원%유선암
high intensity focused ultrasound%tumor%ablation%proliferating cell nuclear antigen%breast cancer
目的 探讨兔乳腺种植瘤经高强度聚焦超声(HIFU)消融后残留肿瘤增殖能力的变化.方法 兔鳞癌细胞株VX＿2肿瘤组织块接种于新西兰兔乳腺,建立兔乳腺种植瘤模型,2周后行HIFU治疗,通过控制探针侧温度,造成肿瘤残留.56只兔子随机分为对照组(n=16)和HIFU组(n=40),经电子显微镜和琥珀酸脱氢酶(SDH)组织化学染色检查确认消融效果和残留肿瘤组织消融后不同时间点检测增殖细胞核抗原(PCNA)的表达情况,同时观察动物生存时间和肿瘤脏器转移的情况.结果 HIFU组消融后,残留肿瘤的PCNA表达呈-过性降低,21 d后恢复至消融前水平.HIFU组的生存时间明显长于对照组,而肺部及腹腔脏器出现转移的时间明显晚于对照组(P<0.05).结论 即使HIFU未能一次完全消融肿瘤,在短时间内也能有效抑制残留肿瘤的生长和转移,延长机体的生存时间.
目的 探討兔乳腺種植瘤經高彊度聚焦超聲(HIFU)消融後殘留腫瘤增殖能力的變化.方法 兔鱗癌細胞株VX＿2腫瘤組織塊接種于新西蘭兔乳腺,建立兔乳腺種植瘤模型,2週後行HIFU治療,通過控製探針側溫度,造成腫瘤殘留.56隻兔子隨機分為對照組(n=16)和HIFU組(n=40),經電子顯微鏡和琥珀痠脫氫酶(SDH)組織化學染色檢查確認消融效果和殘留腫瘤組織消融後不同時間點檢測增殖細胞覈抗原(PCNA)的錶達情況,同時觀察動物生存時間和腫瘤髒器轉移的情況.結果 HIFU組消融後,殘留腫瘤的PCNA錶達呈-過性降低,21 d後恢複至消融前水平.HIFU組的生存時間明顯長于對照組,而肺部及腹腔髒器齣現轉移的時間明顯晚于對照組(P<0.05).結論 即使HIFU未能一次完全消融腫瘤,在短時間內也能有效抑製殘留腫瘤的生長和轉移,延長機體的生存時間.
목적 탐토토유선충식류경고강도취초초성(HIFU)소융후잔류종류증식능력적변화.방법 토린암세포주VX＿2종류조직괴접충우신서란토유선,건립토유선충식류모형,2주후행HIFU치료,통과공제탐침측온도,조성종류잔류.56지토자수궤분위대조조(n=16)화HIFU조(n=40),경전자현미경화호박산탈경매(SDH)조직화학염색검사학인소융효과화잔류종류조직소융후불동시간점검측증식세포핵항원(PCNA)적표체정황,동시관찰동물생존시간화종류장기전이적정황.결과 HIFU조소융후,잔류종류적PCNA표체정-과성강저,21 d후회복지소융전수평.HIFU조적생존시간명현장우대조조,이폐부급복강장기출현전이적시간명현만우대조조(P<0.05).결론 즉사HIFU미능일차완전소융종류,재단시간내야능유효억제잔류종류적생장화전이,연장궤체적생존시간.
Objective To observe the changes in proliferative capability of remnant tumors implanted in rabbit breast after ablation with high intensity focused ultrasound(HIFU). Methods Rabbit implanted models were established by implanting VX_2 tumor mass into the breast of New Zealand rabbits.HIFU was applied to the tumors,and the remnant tumors were harvested by controlling temperature around probe of tumors.Fifty-six rabbits were randomly divided into control group (n=10)and HIFU group(n=40).The expression of proliferating cell nuclear antigen(PCNA)was detected by immunohistochemistry at different time points after ablation,and the survival time and organ metastasis status were observed after ablation. Results After ablation in HIFU group,the positive expression of PCNA was decreased shortly in remnant tumors,and restored to the level before ablation 21 days after treatment.The survival time in HIFU group was much longer than that in control group,and the time of lung and abdominal viscera metastasis in HIFU group appeared significantly later than that in control group(P<0.05). Conclusion Though HIFU ablation can not completely eradicate tumor after one treatment,the growth and metastasis of remnant tumors can be effectively inhibited for a short time,and HIFU ablation can prolong the survival time.
