CAJ | 학술논문

于光镜下对大弹涂鱼性腺切片作了组织学观察,对大弹涂鱼卵细胞和精子发育规律进行研究.结果表明:大弹涂鱼在一个生殖季节中只能产卵1次,大弹涂鱼属于一次性产卵类型.大弹涂鱼3月卵母细胞进入大生长期发育阶段,4-6月为繁殖盛期,7-8月为繁殖末期.10月卵巢基本修整完毕,进入II'恢复期.卵细胞发育可分为6个时相:卵原细胞、卵母细胞单层滤泡、卵母细胞出现脂滴和卵黄、卵母细胞卵黄充满、卵母细胞核极化、卵母细胞退化时相.卵母细胞膜单层,由具有辐射纹的放射带构成,滤泡膜细胞分泌而成的次级卵膜成为成熟卵子的附着丝.大弹涂鱼2月精巢开始发育,5月GSI值达最高值,平均成熟系数达0.70%,排精量最旺盛,出现高峰.7-9月GSI值明显下降.11月至翌年2月GSI值波动于0.08%-0.20%之间,变辐小,此期间精巢处于静止发育状态.大弹涂鱼的精巢属于小叶型结构.精子发育分为6个时期:精原细胞期、精原细胞增殖期、精母细胞生长成熟期、精子细胞变态期、精子成熟期和退化吸收期.繁殖季节精小叶内充满精子,精小囊消失.
우광경하대대탄도어성선절편작료조직학관찰,대대탄도어란세포화정자발육규률진행연구.결과표명:대탄도어재일개생식계절중지능산란1차,대탄도어속우일차성산란류형.대탄도어3월란모세포진입대생장기발육계단,4-6월위번식성기,7-8월위번식말기.10월란소기본수정완필,진입II'회복기.란세포발육가분위6개시상:란원세포、란모세포단층려포、란모세포출현지적화란황、란모세포란황충만、란모세포핵겁화、란모세포퇴화시상.란모세포막단층,유구유복사문적방사대구성,려포막세포분비이성적차급란막성위성숙란자적부착사.대탄도어2월정소개시발육,5월GSI치체최고치,평균성숙계수체0.70%,배정량최왕성,출현고봉.7-9월GSI치명현하강.11월지익년2월GSI치파동우0.08%-0.20%지간,변복소,차기간정소처우정지발육상태.대탄도어적정소속우소협형결구.정자발육분위6개시기:정원세포기、정원세포증식기、정모세포생장성숙기、정자세포변태기、정자성숙기화퇴화흡수기.번식계절정소협내충만정자,정소낭소실.
Observation on the sexual gland of Boleophthalmus pectinirostris was taken. The morphological structures of ooeyte and sperm development of Boleophthalmus pectinirostris were observed by means of light and electron microscopy. According to the histological characteristics of ovary of the fish, The results showed that it spawned once in a reproductive season. The ovaries began to develop in March. Most of the ovaries reached stage IV and stage V respectively from April to June, which was the spawning season period of the fish with highest monthly average of mature coefficient. The highest mature coefficient of an individual reached the value of 11.2%. Spawning behaviour ended in September. In October, the ovary developed into stage II and remained in this stage from October to next February.The oocyte development could be divided into six phases of oogenesis, which were oogonia, oocyte with a single layer of follice cells, oocyte with lipid droplets and yolk globules, oocyte filled with yolk globules, oocyte with migratory nucleus and degenerative oocyte. There was only one layer of membrane surrounding the oocyte whose radiate lines began to appear in the early time of phase 4. The zona radiate grew to its thickest in the late time of phase 4. There was only one fertilization office cell on the membrane. The spermary began to develop in February. Most of the sperms reached maturation stage in May, The highest mature coefficient of an individual reached the value of 0.70%, exhausted sperms were most exuberant, the peak arose. Mature coefficient fell very obviously from July to next September. Mature coefficient (ranging from 0.08%-0.20%) changed very smally and remained in this stage from November to next February, in thie period, spermary was condition of rest. By the microscope, the spermary appeared to have a lobular-type structure, and the sperm development could be divided into six phases of spermatogenesis, which were spermatogonia period, the proliferation of spermatogonia, the growth and maturity of spermatocytes, the abnormality of spermatid, the maturity of sperm and degenerative sperm. There were many sperms in the lobular cavity in the breeding season. The spermatangium would disappear in that time.