中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2010年
4期
316-319
,共4页
王谦%展凤霞%单宁宁%侯明%杨晓静%陆楠%王洪春%张晓琳%冀学斌%纪春岩
王謙%展鳳霞%單寧寧%侯明%楊曉靜%陸楠%王洪春%張曉琳%冀學斌%紀春巖
왕겸%전봉하%단저저%후명%양효정%륙남%왕홍춘%장효림%기학빈%기춘암
转录因子%白细胞介素18%受体,自细胞介素18%免疫性血小板减少症
轉錄因子%白細胞介素18%受體,自細胞介素18%免疫性血小闆減少癥
전록인자%백세포개소18%수체,자세포개소18%면역성혈소판감소증
Transcription factor%Interleukin 18%Receptor,interleukin 18%Immune thrombocytopenia
目的 通过检测新诊断的免疫性血小板减少症(ITP)患者用药前外周血IL-18和CD_3~+细胞表面IL-18受体α链(IL-18Rα)的表达,探讨IL-18和IL-18Rα在ITP发病中的作用机制.方法 以我院门诊及住院治疗的18例新诊断的ITP为研究对象,应用ELISA检测血浆中IL-18的含量,采用流式细胞术分析CD_3~+细胞和总淋巴细胞表面IL-18Rα的表达,应用RT-PCR检测外周血单个核细胞(PBMCs)中IL-18 mRNA、转录因子T-bet mRNA和GATA-3 mRNA的表达.选择15例与试验组匹配的健康志愿者作为正常对照组.结果 新诊断的ITP患者血浆中IL-18的含量为(468.57±141.62)pg/ml,显著高于对照组(P<0.05);CD_3~+细胞表面及淋巴细胞表面IL-18Rα的表达分别为(8.50±3.16)%和(9.16±2.98)%,两者均显著高于对照组(P<0.05);ITP患者PBMCs中IL-18 mRNA、T-bet mRNA和GATA-3 mRNA的表达分别为0.12±0.02、0.07±0.02和0.0039±0.0014,IL-18mRNA和T-bet mRNA显著高于对照组(P<0.05),而GATA-3 mRNA显著低于对照组(P<0.05),T-bet/GATA-3比例显著增高.结论 IL-18与ITP的发生发展有关,本研究从蛋白和基因水平说明IL-18和IL-18Rα可能上调ITP患者Th1类细胞的表达,通过调整T-bet/GATA-3的比例,恢复Th1/Th2的平衡,有望为ITP的治疗提供一个新的治疗策略.
目的 通過檢測新診斷的免疫性血小闆減少癥(ITP)患者用藥前外週血IL-18和CD_3~+細胞錶麵IL-18受體α鏈(IL-18Rα)的錶達,探討IL-18和IL-18Rα在ITP髮病中的作用機製.方法 以我院門診及住院治療的18例新診斷的ITP為研究對象,應用ELISA檢測血漿中IL-18的含量,採用流式細胞術分析CD_3~+細胞和總淋巴細胞錶麵IL-18Rα的錶達,應用RT-PCR檢測外週血單箇覈細胞(PBMCs)中IL-18 mRNA、轉錄因子T-bet mRNA和GATA-3 mRNA的錶達.選擇15例與試驗組匹配的健康誌願者作為正常對照組.結果 新診斷的ITP患者血漿中IL-18的含量為(468.57±141.62)pg/ml,顯著高于對照組(P<0.05);CD_3~+細胞錶麵及淋巴細胞錶麵IL-18Rα的錶達分彆為(8.50±3.16)%和(9.16±2.98)%,兩者均顯著高于對照組(P<0.05);ITP患者PBMCs中IL-18 mRNA、T-bet mRNA和GATA-3 mRNA的錶達分彆為0.12±0.02、0.07±0.02和0.0039±0.0014,IL-18mRNA和T-bet mRNA顯著高于對照組(P<0.05),而GATA-3 mRNA顯著低于對照組(P<0.05),T-bet/GATA-3比例顯著增高.結論 IL-18與ITP的髮生髮展有關,本研究從蛋白和基因水平說明IL-18和IL-18Rα可能上調ITP患者Th1類細胞的錶達,通過調整T-bet/GATA-3的比例,恢複Th1/Th2的平衡,有望為ITP的治療提供一箇新的治療策略.
목적 통과검측신진단적면역성혈소판감소증(ITP)환자용약전외주혈IL-18화CD_3~+세포표면IL-18수체α련(IL-18Rα)적표체,탐토IL-18화IL-18Rα재ITP발병중적작용궤제.방법 이아원문진급주원치료적18례신진단적ITP위연구대상,응용ELISA검측혈장중IL-18적함량,채용류식세포술분석CD_3~+세포화총림파세포표면IL-18Rα적표체,응용RT-PCR검측외주혈단개핵세포(PBMCs)중IL-18 mRNA、전록인자T-bet mRNA화GATA-3 mRNA적표체.선택15례여시험조필배적건강지원자작위정상대조조.결과 신진단적ITP환자혈장중IL-18적함량위(468.57±141.62)pg/ml,현저고우대조조(P<0.05);CD_3~+세포표면급림파세포표면IL-18Rα적표체분별위(8.50±3.16)%화(9.16±2.98)%,량자균현저고우대조조(P<0.05);ITP환자PBMCs중IL-18 mRNA、T-bet mRNA화GATA-3 mRNA적표체분별위0.12±0.02、0.07±0.02화0.0039±0.0014,IL-18mRNA화T-bet mRNA현저고우대조조(P<0.05),이GATA-3 mRNA현저저우대조조(P<0.05),T-bet/GATA-3비례현저증고.결론 IL-18여ITP적발생발전유관,본연구종단백화기인수평설명IL-18화IL-18Rα가능상조ITP환자Th1류세포적표체,통과조정T-bet/GATA-3적비례,회복Th1/Th2적평형,유망위ITP적치료제공일개신적치료책략.
Objective To detect the expression of interleukin (IL)-18 of the peripheral blood cells and IL-18 receptor α chain(IL-18Rα) on the surface of CD_3~+ cells in patients newly diagnosed as immune thrombocytopenia (ITP) before medication and to explore the roles of IL-18 and IL-18Rα in the development of ITP. Methods Eighteen out-patients or inpatients with acute ITP accepting treatment in Qilu Hospital were enrolled in this study and 15 matching healthy subjects were taken as control. Plasma IL-18 level was detected with enzyme linked immunosorbent assay (ELISA), the expression of IL-18Rα on CD_3~+ lymphocytes and total lymphoeytes were measured with flow cytometry; T-bet and GATA-3 mRNA were measured with reverse transcriptase polymcrase chain reaction (RT-PCR). Results The expression of IL-18 in acute ITP plasma was (468. 57 ± 141.62) ng/L and IL-18Rα on the surface of CD_3~+ cells and lymphocytes were (8.50 ±3. 16)% and (9. 16±2.98)% respectively. The levels of IL-18 and IL-18Rα were increased in active ITP patients as compared with those in the controls (P <0. 05). The levels of IL-18 mRNA (0. 12 ±0. 02) and T-bet mRNA (0. 07 ±0. 02) were significantly increased in patients with active ITP as compared with those in the controls (P <0.05), while GATA-3 mRNA (0.0039±0.0014) were significantly decreased in patients with active ITP (P < 0. 05). The balance between T-bet and GATA-3 was significantly disturbed in ITP. Conclusions Through the variation of the levels of gene and protein, our study showed that IL-18 and IL-18Rα might upregulate the expression of Th1-cytokines in ITP patients. It is also suggested that IL-18 has potential association with the development of ITP. Especially, it may provide a new treatment method for ITP by regulating the ratio of T-bet and GATA-3 and resuming the balance of Th1/ Th2.
