中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
5期
267-271
,共5页
林静%赵今%努尔比亚木·麦麦提依明%李新尚
林靜%趙今%努爾比亞木·麥麥提依明%李新尚
림정%조금%노이비아목·맥맥제의명%리신상
维吾尔族%链球菌,变异%基因型%随机引物聚合酶链反应
維吾爾族%鏈毬菌,變異%基因型%隨機引物聚閤酶鏈反應
유오이족%련구균,변이%기인형%수궤인물취합매련반응
Uygur nationality%Streptococcus mutans%Genotype%Arbitrarily primed polymerase chsin reaction
目的 探讨新疆维吾尔族不同龋敏感儿童口腔中变形链球菌幕冈多态性与乳才龋发生的关系,以期为新疆维吾尔族儿童乳牙龋危险性预测和防治提供依据.方法 选取新疆乌鲁小齐市3～5岁维吾尔族高龋(龋失补牙数≥5)和无龋(龋失补牙数=0)儿童各17名为研究对象,收集唾液样本,轻唾一杆菌肽琼脂培养基分离培养变形链球菌.典型菌落行革兰染色、生化鉴定并细菌保种,提取基因组DNA,采用OPA02、OPA05和OPA13三个随机引物对变形链球菌临床分离株行随机引物聚合酶链反应基因分型及聚类分析.结果 分别从高龋和无龋儿童口腔中分离鉴定变形链球菌81和62株,共得到40种不同的基因型.17例高龋儿童中9例携带1种基因型,5例携带2种基因型,3例携带3种基因型;17名无龋儿童中14名携带1种基因型,3名携带2种以上基因型.Spearman相关结果 显示基因多态性与龋敏感相关(r=0.342,P<0.05).结论 维吾尔族儿童口腔中变形链球菌菌株间存在明显的基因多态性,高龋儿童口腔中定植的变形链球菌有多种基因型,个体携带基因型的种类数与其致龋能力相关.
目的 探討新疆維吾爾族不同齲敏感兒童口腔中變形鏈毬菌幕岡多態性與乳纔齲髮生的關繫,以期為新疆維吾爾族兒童乳牙齲危險性預測和防治提供依據.方法 選取新疆烏魯小齊市3～5歲維吾爾族高齲(齲失補牙數≥5)和無齲(齲失補牙數=0)兒童各17名為研究對象,收集唾液樣本,輕唾一桿菌肽瓊脂培養基分離培養變形鏈毬菌.典型菌落行革蘭染色、生化鑒定併細菌保種,提取基因組DNA,採用OPA02、OPA05和OPA13三箇隨機引物對變形鏈毬菌臨床分離株行隨機引物聚閤酶鏈反應基因分型及聚類分析.結果 分彆從高齲和無齲兒童口腔中分離鑒定變形鏈毬菌81和62株,共得到40種不同的基因型.17例高齲兒童中9例攜帶1種基因型,5例攜帶2種基因型,3例攜帶3種基因型;17名無齲兒童中14名攜帶1種基因型,3名攜帶2種以上基因型.Spearman相關結果 顯示基因多態性與齲敏感相關(r=0.342,P<0.05).結論 維吾爾族兒童口腔中變形鏈毬菌菌株間存在明顯的基因多態性,高齲兒童口腔中定植的變形鏈毬菌有多種基因型,箇體攜帶基因型的種類數與其緻齲能力相關.
목적 탐토신강유오이족불동우민감인동구강중변형련구균막강다태성여유재우발생적관계,이기위신강유오이족인동유아우위험성예측화방치제공의거.방법 선취신강오로소제시3～5세유오이족고우(우실보아수≥5)화무우(우실보아수=0)인동각17명위연구대상,수집타액양본,경타일간균태경지배양기분리배양변형련구균.전형균락행혁란염색、생화감정병세균보충,제취기인조DNA,채용OPA02、OPA05화OPA13삼개수궤인물대변형련구균림상분리주행수궤인물취합매련반응기인분형급취류분석.결과 분별종고우화무우인동구강중분리감정변형련구균81화62주,공득도40충불동적기인형.17례고우인동중9례휴대1충기인형,5례휴대2충기인형,3례휴대3충기인형;17명무우인동중14명휴대1충기인형,3명휴대2충이상기인형.Spearman상관결과 현시기인다태성여우민감상관(r=0.342,P<0.05).결론 유오이족인동구강중변형련구균균주간존재명현적기인다태성,고우인동구강중정식적변형련구균유다충기인형,개체휴대기인형적충류수여기치우능력상관.
Objective To investigate the relationship between genotypic diversity of Streptococcus mutans(Sm)and deciduous teeth caries in Xinjiang Uygur autonomous region.Methods Isolates of Sm were obtained from 17 caries-free and 17 caries-active Uygur preschool children aged from 3 to 5 years.A total of 143 isolates were subcultured,biochemically characterised and identified by polymerase chain reaction(PCR)as Sm and Streptococcus sobrinus(Ss).Then the Sm isolates were genotyped by arbitrarily primed-PCR(AP-PCR).Results A total of 81 Sm isolates from caries-active subjects and 62 isolates from caries-free subjects were identified by PCR and forty distinct genotypes identified from 143 clinicalisolates.In seventeen caries-active group,9 children had only 1 genotype,5 children had 2 genotypes,3 children had 3 genotypes.In seventeen caries-free group,14 children had only 1 genotype,3 children had more than 2 genotypes.The Spearman correlation test showed a strong associatiou between genotypic diversity and caries experience(r=0.342.P<0.05).Conclusions Isolates of Sm in caries-active Uygur preschool children show apparent more genetic diversity than those in caries-free children.The genotypes of isolates might be related to differences in caries susceptibility.
