CAJ | 학술논문

目的研究Beagle犬静脉注射乳化异氟醚的急性毒性.方法健康成年Beagle犬6只,雌雄各半,月龄6～8月,体重6～8 kg,设定乳化异氟醚注射液(120 mg/ml)剂量序列为1.2、1.5、1.8、2.1、2.4、2.7、3.0、3.3、3.6、3.9 ml/kg.用30%脂肪乳定容至4.0 ml/kg,注射时间60 s,以3.0 ml/kg为初始剂量,采用间隔剂量给药的方法,注射后犬死亡,则下一只犬间隔一个低剂量给药,注射后犬未死亡,则下一只犬间隔一个高剂量给药,确定最大耐受剂量(MTD)和最低致死剂量,然后用这两个剂量之间的剂量[近似致死剂量(ALD)]给1只犬注射.观察毒性反应,死亡犬作大体解剖,体积、颜色、纹理改变的器官作组织病理学检查.结果 Beagle犬静脉注射乳化异氟醚的ALD和MTD分别为252、216 mg/kg,其急性毒性反应主要表现为BP降低,呼吸变浅、变慢,HR降低,出现室颤.死亡犬的大体解剖和各脏器组织病理学检查结果均未见异常.结论 Beagle犬静脉注射乳化异氟醚的ALD和MTD分别为252、216 mg/kg,其急性毒性反应主要表现为呼吸和循环功能抑制.
목적 연구Beagle견정맥주사유화이불미적급성독성.방법 건강성년Beagle견6지,자웅각반,월령6～8월,체중6～8 kg,설정유화이불미주사액(120 mg/ml)제량서렬위1.2、1.5、1.8、2.1、2.4、2.7、3.0、3.3、3.6、3.9 ml/kg.용30%지방유정용지4.0 ml/kg,주사시간60 s,이3.0 ml/kg위초시제량,채용간격제량급약적방법,주사후견사망,칙하일지견간격일개저제량급약,주사후견미사망,칙하일지견간격일개고제량급약,학정최대내수제량(MTD)화최저치사제량,연후용저량개제량지간적제량[근사치사제량(ALD)]급1지견주사.관찰독성반응,사망견작대체해부,체적、안색、문리개변적기관작조직병이학검사.결과 Beagle견정맥주사유화이불미적ALD화MTD분별위252、216 mg/kg,기급성독성반응주요표현위BP강저,호흡변천、변만,HR강저,출현실전.사망견적대체해부화각장기조직병이학검사결과균미견이상.결론 Beagle견정맥주사유화이불미적ALD화MTD분별위252、216 mg/kg,기급성독성반응주요표현위호흡화순배공능억제.
Objective To investigate the acute toxicity of intravenous isoflurane in Beagles.Methods Six healthy adult Beagles of both sexes aged 6-8 months weighing 6-8 kg were used in this study.Isoflurane injectio (120 mg/ml) in 30% hpid emulsion was injected intravenously. Femoral artery was cannulated for direct BP monitoring.ECG was continuously monitored.The maximal tolerance dose (MTD) and approximate lethal dose (ALD) were determined by up-and-down technique. The initial dose was 3.0 ml/kg. The dose was decreased/increased by 0.3 ml/kg if the previous animal died/survived.The survived dogs were observed for 2 weeks.Autopsy and histopathological examination were performed on all dead Beagles.Results The ALD and MTD of intravenous isoflurane were 252 and 216 mg/kg. Autopsy and histopathological examination did not show any abnormality.Conclusion Cardiopulmonary depression is the main manifestation of the acute toxicity of intravenous isoflurane in Beagles.