CAJ | 학술논문

目的探讨儿童急性淋巴细胞白血病(ALL)免疫表型特征和诊断价值.方法应用直接免疫荧光标记以CD45/SSC双参数散点图设门法对111例初治儿童ALL行流式细胞术分析,并探讨形态学分型与免疫分型的关系.结果本组ALL免疫表型特点:①B系ALL 81例(73.0%),T系ALL 16例(14.4%),B/T-ALL 14例(12.6%);②伴髓系抗原表达者(My+-ALL)25例(22.5%);③FAB分型L1型59例(53.2%),L2型47例(42.3%),L3型2例(1.8%,Ⅰ型旱前B型和成熟B型各1例).急性粒细胞白血病(M2a)1例(0.9%,为Ⅰ型早期前B型ALL),形态学无法分类2例(均为Ⅱ型早前B型ALL).结论免疫分型有助于明确白血病性质,是儿童ALL诊断的必要手段.
목적 탐토인동급성림파세포백혈병(ALL)면역표형특정화진단개치.방법 응용직접면역형광표기이CD45/SSC쌍삼수산점도설문법대111례초치인동ALL행류식세포술분석,병탐토형태학분형여면역분형적관계.결과 본조ALL면역표형특점:①B계ALL 81례(73.0%),T계ALL 16례(14.4%),B/T-ALL 14례(12.6%);②반수계항원표체자(My+-ALL)25례(22.5%);③FAB분형L1형59례(53.2%),L2형47례(42.3%),L3형2례(1.8%,Ⅰ형한전B형화성숙B형각1례).급성립세포백혈병(M2a)1례(0.9%,위Ⅰ형조기전B형ALL),형태학무법분류2례(균위Ⅱ형조전B형ALL).결론 면역분형유조우명학백혈병성질,시인동ALL진단적필요수단.
Objective To study the characteristics of child acute lymphocytic leukemia (ALL) immuno-phenotype and evaluate its diagnosis value. Methods Direct immunofluorescence staining and CD45/Side Scatter (SSC) gating of flow cytometry were used for immunophenotyping in 111 cases of child ALL. The relation of mor-phology and immunology classification was analyzed. Results Three categories could be identified,including 81 ca-ses (73.0%) of B lineage ALL, 16 cases (14.4%) of T lineage ALL and 14 cases (12.6% ) of B/T lineage ALL. There were 25 cases (22.5% ) of ALL expressing myeloid-associated antigens. According to the FAB Morphology classification,59 cases (53.2%) of L1 type and 47 cases (42.3%) of L2 type were diagnosed. The two cases (1.8%) of L3 type were classified as one case of null-ALL and one case of B-ALL. One case (0.9%)of acute my-eloblastic leukemia (AML-M2a) was identified as null-ALL. The two cases that could not be diagnosed by FAB clas-sification were c-ALL. Conclusion The immunophenotyping helps to identify the character of leukemia with an im-portant value in diagnosis of child ALL.