CAJ | 학술논문

目的评价贝伐单抗(avastin)局部应用对小鼠角膜新生血管(CNV)的抑制作用.方法通过碱烧伤建立CNV模型,将30只Balb/c小鼠随机分成5组,A组贝伐单抗 1mg/Ml每日点眼2次;B组贝伐单抗 3mg/Ml每日点眼2次;C组贝伐单抗 5mg/Ml每日点眼2次;D组0.1%地塞米松每日点眼2次;E组生理盐水每日点眼2次.分别于术后3、7、14d观察CNV情况并拍照.术后第14天,处死全部小鼠,行CNV内皮细胞荧光标记,计算CNV所占全角膜面积的比例.结果各组CNV面积为A组(37.11±3.17)%、B组(29.75±3.56)%、C组(18.76±2.55)%、D组(20.91±2.75)%,E组(41.65±2.11)%.各组小鼠CNV面积依次为C组＜D组＜B组＜A组＜E组,C组同A、B、E组比较差异均有统计学意义(P＜0.01),C组与D组比较差异无统计学意义(P=0.694).结论局部应用贝伐单抗对小鼠角膜化学烧伤后的CNV有抑制作用.
목적 평개패벌단항(avastin)국부응용대소서각막신생혈관(CNV)적억제작용.방법 통과감소상건립CNV모형,장30지Balb/c소서수궤분성5조,A조패벌단항 1mg/Ml매일점안2차;B조패벌단항 3mg/Ml매일점안2차;C조패벌단항 5mg/Ml매일점안2차;D조0.1%지새미송매일점안2차;E조생리염수매일점안2차.분별우술후3、7、14d관찰CNV정황병박조.술후제14천,처사전부소서,행CNV내피세포형광표기,계산CNV소점전각막면적적비례.결과 각조CNV면적위A조(37.11±3.17)%、B조(29.75±3.56)%、C조(18.76±2.55)%、D조(20.91±2.75)%,E조(41.65±2.11)%.각조소서CNV면적의차위C조＜D조＜B조＜A조＜E조,C조동A、B、E조비교차이균유통계학의의(P＜0.01),C조여D조비교차이무통계학의의(P=0.694).결론 국부응용패벌단항대소서각막화학소상후적CNV유억제작용.
Objective The inhibitory effects of avastin on new blood vessels in nonproliferation diabetic retinopathy, age-related macular degeneration and neovascular glaucoma have been demonstrated. But only seldom report of avastin on corneal neovascularization(CNV) was seen. Present study was to evaluate the effect of topical bevacizumab (avastin) on experimental corneal neovascularization in mice. Methods Thirty eyes of 30 Balb/c mice were chemically cauterized by applying a 2 mm-diameter filter paper soaked 1 mol/L NaOH solution at the central cornea for 40 s. All animals were randomly assigned to five groups, including 1 mg/mL, 3 mg/mL and 5 mg/mL bevacizumab eye drops group respectively, 1 mg/mL dexamethasone sodium phosphate eye drops group (positive control) and normal saline solution group (negative control) . The drug was topically utilized twice per day. CNV was examined under the slim lamp on the 3rd, 7th and 14th day after alkali burn. Animals were killed on the 14th day after alkali burn. Area of CNV was calculated in terms of pixels on digital photographs. The use of animals followed the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results No significant difference was found in the grade of corneal injury among five groups (F = 0. 201, P = 0. 935). The area of neovascularization at the cornea surface was (37.11 ±3.17)% in 1 mg/mL bevacizumab group, (29.75 ±3.56)% in 3 mg/mL bevacizumab group, (18. 76 ± 2. 55) % in 5 mg/mL bevacizumab group, (20. 91 ± 2. 75) % in dexamethasone group and (41. 65 ±2. 11)% in normal saline group, showing a significant difference among groups(F = 71. 687, P =0. 000) with the further comparative decline in 5 mg/mL bevacizumab group compared with other groups (P ＜ 0. 01) . Conclusion The topical use of bevacizumab (avastin) inhibits alkali burn-induced CNV in mice.