中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2012年
4期
256-260
,共5页
何彦安%雷正明%叶明新%唐华明%付文广%向鑫
何彥安%雷正明%葉明新%唐華明%付文廣%嚮鑫
하언안%뢰정명%협명신%당화명%부문엄%향흠
胆囊%胆结石%胆汁%受体,G-蛋白偶联
膽囊%膽結石%膽汁%受體,G-蛋白偶聯
담낭%담결석%담즙%수체,G-단백우련
Gallbladder%Cholelithiasis%Bile%Receptors,G-protein-coupled
目的 研究胆囊结石患者胆囊黏膜G蛋白偶联胆汁酸受体1 (GPBAR1)表达与致石胆汁形成的关系.方法 收集34例胆囊结石病和15例无胆石对照患者的胆囊黏膜、囊壁、胆汁、静脉血.HE常规染色病理检查胆囊壁,免疫组化染色法检测胆囊壁GPBAR1、黏蛋白1(MUC1)和黏蛋白5AC(MUC5AC)的表达水平；RT-PCR反应检测胆囊黏膜GPBAR1、MUC1和MUC5AC的基因表达水平；检测术前血清和胆囊胆汁的主要脂质成分等.结果 胆囊结石组胆囊HE染色均呈慢性炎症表现；胆囊结石组GPBAR1、MUC5AC的蛋白和mRNA表达均较对照组明显升高(61.34±8.06比43.05±7.83,P＜0.01; 52.11±9.62比45.05±9.27,P＜0.05;0.87±0.07比0.80±0.09,P＜0.05；1.04±0.22比0.8±0.17,P＜0.01).胆囊结石组血清总胆固醇水平和胆汁总胆固醇浓度、总胆固醇摩尔百分比、胆固醇饱和指数、黏蛋白浓度均较对照组明显升高(5.07±1.64比3.62±1.42,P＜0.01;17.23±3.67比12.47±2.31,P＜0.01;7.47±0.65比5.05±0.24,P＜0.01;1.03±0.58比0.69±0.38,P＜0.01；92.02±20.89比76.36±19.71,P＜0.05)；而胆汁总胆汁酸浓度、胆汁酸摩尔百分比较对照组明显降低(162.68±20.19比180.21±26.05,P＜0.05; 71.28±1.84比73.29±0.96,P＜0.01).胆囊结石组胆囊GPBAR1 mRNA表达与胆汁总胆汁酸水平负相关(γ=-0.341,P＜0.05),GPBAR1表达与胆汁总胆汁酸水平和总脂质水平均负相关(γ=-0.365,P＜0.05;γ=-0.403,P＜0.05).结论 GPBAR1在胆囊结石患者胆囊黏膜表达增强,介导胆汁酸吸收,可能与致石胆汁形成有关.
目的 研究膽囊結石患者膽囊黏膜G蛋白偶聯膽汁痠受體1 (GPBAR1)錶達與緻石膽汁形成的關繫.方法 收集34例膽囊結石病和15例無膽石對照患者的膽囊黏膜、囊壁、膽汁、靜脈血.HE常規染色病理檢查膽囊壁,免疫組化染色法檢測膽囊壁GPBAR1、黏蛋白1(MUC1)和黏蛋白5AC(MUC5AC)的錶達水平；RT-PCR反應檢測膽囊黏膜GPBAR1、MUC1和MUC5AC的基因錶達水平；檢測術前血清和膽囊膽汁的主要脂質成分等.結果 膽囊結石組膽囊HE染色均呈慢性炎癥錶現；膽囊結石組GPBAR1、MUC5AC的蛋白和mRNA錶達均較對照組明顯升高(61.34±8.06比43.05±7.83,P＜0.01; 52.11±9.62比45.05±9.27,P＜0.05;0.87±0.07比0.80±0.09,P＜0.05；1.04±0.22比0.8±0.17,P＜0.01).膽囊結石組血清總膽固醇水平和膽汁總膽固醇濃度、總膽固醇摩爾百分比、膽固醇飽和指數、黏蛋白濃度均較對照組明顯升高(5.07±1.64比3.62±1.42,P＜0.01;17.23±3.67比12.47±2.31,P＜0.01;7.47±0.65比5.05±0.24,P＜0.01;1.03±0.58比0.69±0.38,P＜0.01；92.02±20.89比76.36±19.71,P＜0.05)；而膽汁總膽汁痠濃度、膽汁痠摩爾百分比較對照組明顯降低(162.68±20.19比180.21±26.05,P＜0.05; 71.28±1.84比73.29±0.96,P＜0.01).膽囊結石組膽囊GPBAR1 mRNA錶達與膽汁總膽汁痠水平負相關(γ=-0.341,P＜0.05),GPBAR1錶達與膽汁總膽汁痠水平和總脂質水平均負相關(γ=-0.365,P＜0.05;γ=-0.403,P＜0.05).結論 GPBAR1在膽囊結石患者膽囊黏膜錶達增彊,介導膽汁痠吸收,可能與緻石膽汁形成有關.
목적 연구담낭결석환자담낭점막G단백우련담즙산수체1 (GPBAR1)표체여치석담즙형성적관계.방법 수집34례담낭결석병화15례무담석대조환자적담낭점막、낭벽、담즙、정맥혈.HE상규염색병리검사담낭벽,면역조화염색법검측담낭벽GPBAR1、점단백1(MUC1)화점단백5AC(MUC5AC)적표체수평；RT-PCR반응검측담낭점막GPBAR1、MUC1화MUC5AC적기인표체수평；검측술전혈청화담낭담즙적주요지질성분등.결과 담낭결석조담낭HE염색균정만성염증표현；담낭결석조GPBAR1、MUC5AC적단백화mRNA표체균교대조조명현승고(61.34±8.06비43.05±7.83,P＜0.01; 52.11±9.62비45.05±9.27,P＜0.05;0.87±0.07비0.80±0.09,P＜0.05；1.04±0.22비0.8±0.17,P＜0.01).담낭결석조혈청총담고순수평화담즙총담고순농도、총담고순마이백분비、담고순포화지수、점단백농도균교대조조명현승고(5.07±1.64비3.62±1.42,P＜0.01;17.23±3.67비12.47±2.31,P＜0.01;7.47±0.65비5.05±0.24,P＜0.01;1.03±0.58비0.69±0.38,P＜0.01；92.02±20.89비76.36±19.71,P＜0.05)；이담즙총담즙산농도、담즙산마이백분비교대조조명현강저(162.68±20.19비180.21±26.05,P＜0.05; 71.28±1.84비73.29±0.96,P＜0.01).담낭결석조담낭GPBAR1 mRNA표체여담즙총담즙산수평부상관(γ=-0.341,P＜0.05),GPBAR1표체여담즙총담즙산수평화총지질수평균부상관(γ=-0.365,P＜0.05;γ=-0.403,P＜0.05).결론 GPBAR1재담낭결석환자담낭점막표체증강,개도담즙산흡수,가능여치석담즙형성유관.
Objective To study the relationship between expression of G-protein-coupled bile acid receptor 1 (GPBAR1) in gallbladder mucosa and formation of lithogenic bile in patients with gallstones.Methods Gallbladder mucosa,gallbladder wall,bile and plasma were collected from 34 patients with gallstone (GS) and 15 individuals who were gallstone free (GSF).The gallbladder wall was stained with hematoxylin-eosin (HE) and immunohistochemistry to detect pathologic changes and expressions of GPBAR1,mucin 1 (MUC1) and mucin 5AC (MUC5AC).Reverse-transcription polymerase chain reaction (RT-PCR) was used to test mRNA expressions of GPBAR1,MUC1 and MUC5AC in the gallbladder mucosa.The contents of total cholesterol (TC),total bile acid (TBA),triglyceride (TG),low density lipoprotein (LDL) and high density lipoprotein (HDL) in plasma and cholesterol,TBA,phospholipid (PL) and mucin in the bile of gallbladder were measured.Results The gallbladder mucosa in all GS patients showed chronic inflammation on hematoxylin-eosin staining.The expressions of GPBAR1 and MUC5AC were more markedly increased in the GS group than in the GSFgroup (61.34±8.06 vs.43.05±7.83,P＜0.01; 52.11±9.62 vs.45.05±9.27,P＜0.05).The mRNA expressions of GPBAR1 and MUC5AC in the GS group were also more markedly increased than in the GSR group (0.87±0.07 vs.0.80±0.09,P＜0.05; 1.04±0.22 vs.0.8±0.17,P＜0.01).Serum cholesterol,as well as biliary cholesterol,cholesterol mol percentage,cholesterol saturation index and mucin in the GS group were more significantly higher than in the GSF group (5.07±1.64 vs.3.62±1.42,P＜0.01; 17.23±3.67 vs.12.47±2.31,P＜0.01; 7.47±0.65 vs.5.05±0.24,P＜0.01; 1.03±0.58 vs.0.69±0.38,P＜0.01; 92.02±20.89 vs.76.36±19.71,P＜0.05).Biliary total bile acids and bile acids mol percentage were lower in the GS group than in the GSF group (162.68±20.19 vs.180.21±26.05,P＜0.05; 71.28±1.84 vs. 73.29±0.96,P＜0.01). In the GS group,there were negative correlations between the mRNA expression of GPBAR1 and biliary TBA (γ=-0.341,P＜0.05).There were negative correlations in the GS group between the GPBAR1 expression and the level of biliary TBA (γ=- 0.403,P＜0.05),and between the GPBAR1 expression and the level of biliary total lipid (γ=-0.365,P＜0.05).Conclusions This study shows an increase in expression of GPBAR1 in gallbladder mucosa in patients with GS.It is suggested that GPBAR1 may accelerate formation of lithogenic bile by inducing re-absorption of bile acid.
