中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2012年
1期
33-38
,共6页
张银丽%郭小荣%沈丹华%成夜霞%粱旭东%陈云新%王颖
張銀麗%郭小榮%瀋丹華%成夜霞%粱旭東%陳雲新%王穎
장은려%곽소영%침단화%성야하%량욱동%진운신%왕영
卵巢肿瘤%肿瘤抑制蛋白质p53%寡核苷酸序列分析%免疫组织化学%甲基化
卵巢腫瘤%腫瘤抑製蛋白質p53%寡覈苷痠序列分析%免疫組織化學%甲基化
란소종류%종류억제단백질p53%과핵감산서렬분석%면역조직화학%갑기화
Ovarian neoplasms%Tumor suppressor protein p53%Oligonucleotide array sequence analysis%Immunohistochemistry%Methylation
目的 探讨卵巢上皮性肿瘤中p73蛋白的表达和基因启动子的甲基化情况,并观察其与临床病理学特征的关系.方法 制备包括68例卵巢癌、37例卵巢交界性肿瘤和21例卵巢良性肿瘤的组织芯片,用免疫组织化学EnVision法检测上述组织中p73蛋白表达情况,用亚硫酸氧盐修饰后测序法检测13例新鲜卵巢癌组织及5例新鲜卵巢交界性肿瘤组织的p73基因启动子甲基化情况.结果 92.6％ (63/68)的卵巢癌表达p73,p73蛋白总体表达率均值为32％(p73表达率指p73阳性细胞数所占的百分比),其中浆液性癌( 26/26)的表达率均值为40％,高于其他组织类型的癌(P=0.006).按照卵巢癌发病模式区分,Ⅱ型卵巢癌p73表达率均值(40％)高于Ⅰ型卵巢癌(24％),P=0.010.卵巢癌中p73的表达与临床分期及组织学分级无相关性(均P＞0.05).卵巢交界性肿瘤组(30/37)和良性肿瘤组(12/21)p73的总体表达率均值分别为16％和15％,该两组肿瘤中浆液性肿瘤表达率均值均高于黏液性肿瘤(P-0.003,P=0.026).卵巢癌组的p73阳性表达率均值明显高于交界性肿瘤组和良性肿瘤组(均P ＜0.05),交界性肿瘤组与良性肿瘤组比较差异无统计学意义(P＞0.05).浆液性肿瘤( 49/53)中,卵巢癌组(26/26) p73阳性表达率均值明显高于交界性肿瘤组(12/14)和良性肿瘤组(11/13；P =0.024和P=0.002),而卵巢交界性肿瘤组和良性肿瘤组比较差异无统计学意义(P=0.428).黏液性肿瘤(15/27)中,卵巢癌组(6/7)p73阳性表达率均值高于良性肿瘤组( 1/8；p=0.032),而卵巢癌组与卵巢交界性肿瘤组(8/12)、交界性肿瘤组与良性肿瘤组比较,差异均无统计学意义(P=0.234和P=0.201).p73启动子的甲基化结果显示,13例卵巢癌有8例发生甲基化,但每例样本甲基化频率有所不同,总体甲基化频率均值为8.0％.5例交界性肿瘤有2例发生甲基化,总体甲基化频率均值为9.0％,两组比较差异无统计学意义(P＞0.05).卵巢癌组p73甲基化额率与组织类型、发病模式、组织学分级及临床分期均无相关性(均P＞0.05).结论 卵巢上皮性肿瘤多数表达p73,卵巢癌p73的表达率均值明显高于交界性肿瘤和良性肿瘤,浆液性肿瘤高于其他组织类型；p73蛋白表达率与p73基因甲基化程度不存在简单线性相关关系.
目的 探討卵巢上皮性腫瘤中p73蛋白的錶達和基因啟動子的甲基化情況,併觀察其與臨床病理學特徵的關繫.方法 製備包括68例卵巢癌、37例卵巢交界性腫瘤和21例卵巢良性腫瘤的組織芯片,用免疫組織化學EnVision法檢測上述組織中p73蛋白錶達情況,用亞硫痠氧鹽脩飾後測序法檢測13例新鮮卵巢癌組織及5例新鮮卵巢交界性腫瘤組織的p73基因啟動子甲基化情況.結果 92.6％ (63/68)的卵巢癌錶達p73,p73蛋白總體錶達率均值為32％(p73錶達率指p73暘性細胞數所佔的百分比),其中漿液性癌( 26/26)的錶達率均值為40％,高于其他組織類型的癌(P=0.006).按照卵巢癌髮病模式區分,Ⅱ型卵巢癌p73錶達率均值(40％)高于Ⅰ型卵巢癌(24％),P=0.010.卵巢癌中p73的錶達與臨床分期及組織學分級無相關性(均P＞0.05).卵巢交界性腫瘤組(30/37)和良性腫瘤組(12/21)p73的總體錶達率均值分彆為16％和15％,該兩組腫瘤中漿液性腫瘤錶達率均值均高于黏液性腫瘤(P-0.003,P=0.026).卵巢癌組的p73暘性錶達率均值明顯高于交界性腫瘤組和良性腫瘤組(均P ＜0.05),交界性腫瘤組與良性腫瘤組比較差異無統計學意義(P＞0.05).漿液性腫瘤( 49/53)中,卵巢癌組(26/26) p73暘性錶達率均值明顯高于交界性腫瘤組(12/14)和良性腫瘤組(11/13；P =0.024和P=0.002),而卵巢交界性腫瘤組和良性腫瘤組比較差異無統計學意義(P=0.428).黏液性腫瘤(15/27)中,卵巢癌組(6/7)p73暘性錶達率均值高于良性腫瘤組( 1/8；p=0.032),而卵巢癌組與卵巢交界性腫瘤組(8/12)、交界性腫瘤組與良性腫瘤組比較,差異均無統計學意義(P=0.234和P=0.201).p73啟動子的甲基化結果顯示,13例卵巢癌有8例髮生甲基化,但每例樣本甲基化頻率有所不同,總體甲基化頻率均值為8.0％.5例交界性腫瘤有2例髮生甲基化,總體甲基化頻率均值為9.0％,兩組比較差異無統計學意義(P＞0.05).卵巢癌組p73甲基化額率與組織類型、髮病模式、組織學分級及臨床分期均無相關性(均P＞0.05).結論 卵巢上皮性腫瘤多數錶達p73,卵巢癌p73的錶達率均值明顯高于交界性腫瘤和良性腫瘤,漿液性腫瘤高于其他組織類型；p73蛋白錶達率與p73基因甲基化程度不存在簡單線性相關關繫.
목적 탐토란소상피성종류중p73단백적표체화기인계동자적갑기화정황,병관찰기여림상병이학특정적관계.방법 제비포괄68례란소암、37례란소교계성종류화21례란소량성종류적조직심편,용면역조직화학EnVision법검측상술조직중p73단백표체정황,용아류산양염수식후측서법검측13례신선란소암조직급5례신선란소교계성종류조직적p73기인계동자갑기화정황.결과 92.6％ (63/68)적란소암표체p73,p73단백총체표체솔균치위32％(p73표체솔지p73양성세포수소점적백분비),기중장액성암( 26/26)적표체솔균치위40％,고우기타조직류형적암(P=0.006).안조란소암발병모식구분,Ⅱ형란소암p73표체솔균치(40％)고우Ⅰ형란소암(24％),P=0.010.란소암중p73적표체여림상분기급조직학분급무상관성(균P＞0.05).란소교계성종류조(30/37)화량성종류조(12/21)p73적총체표체솔균치분별위16％화15％,해량조종류중장액성종류표체솔균치균고우점액성종류(P-0.003,P=0.026).란소암조적p73양성표체솔균치명현고우교계성종류조화량성종류조(균P ＜0.05),교계성종류조여량성종류조비교차이무통계학의의(P＞0.05).장액성종류( 49/53)중,란소암조(26/26) p73양성표체솔균치명현고우교계성종류조(12/14)화량성종류조(11/13；P =0.024화P=0.002),이란소교계성종류조화량성종류조비교차이무통계학의의(P=0.428).점액성종류(15/27)중,란소암조(6/7)p73양성표체솔균치고우량성종류조( 1/8；p=0.032),이란소암조여란소교계성종류조(8/12)、교계성종류조여량성종류조비교,차이균무통계학의의(P=0.234화P=0.201).p73계동자적갑기화결과현시,13례란소암유8례발생갑기화,단매례양본갑기화빈솔유소불동,총체갑기화빈솔균치위8.0％.5례교계성종류유2례발생갑기화,총체갑기화빈솔균치위9.0％,량조비교차이무통계학의의(P＞0.05).란소암조p73갑기화액솔여조직류형、발병모식、조직학분급급림상분기균무상관성(균P＞0.05).결론 란소상피성종류다수표체p73,란소암p73적표체솔균치명현고우교계성종류화량성종류,장액성종류고우기타조직류형；p73단백표체솔여p73기인갑기화정도불존재간단선성상관관계.
Objective To investigate the expression and promoter methylation status of p73 gene in ovarian epithelial tumors and their clinicopathological correlations.Methods Tissue microarrays ( TMA )consisting of 68 ovarian cancers,37 ovarian borderline tumors and 21 ovarian benign tumors were constructed.p73 expression was detected by immunohistochemistry (EnVision method).Fresh-frozen tissue samples from 13 cases of ovarian carcinomas and 5 cases of borderline tumors were evaluated for the presence of p73 promoter methylation using bisulfite sequencing.Results Overall,92.6％ (63/68) ovarian carcinomas expressed p73,with a mean value of 32％ (percentage of p73 positive cells in the tumor).The mean value of p73 expression rate (40％) in serous carcinoma ( 26/26 ) was higher than those of other cancer types (P =0.006),The mean value of p73 expression rate (40％) in type Ⅱ ovarian carcinoma was significantly higher than that in type Ⅰ ovarian carcinoma (24％,P =0.010).The expression of p73 was not associated with FIGO stage and histological grade ( both P ＞ 0.05 ).The mean values of p73 expression in ovarian borderline tumor (30/37) and benign tumor ( 12/21 ) were 16％ and 15％,respectively.Of the two groups,the mean value of p73 expression rate in serous type was higher than that in mucous type (P =0.003,P=0.026).Ovarian carcinomas had a higher level of p73 expression than borderline tumors and benign tumors ( both P ＜ 0.05 ),while that between ovarian borderline tumors and benign tumors had no statistical difference ( P ＞ 0.05 ).Among serous tumors (49/53),the mean value of p73 expression in the carcinoma group (26/26) was significantly higher than those in the borderline tumor group (12/14) and benign tumor group ( 11/13 ; P =0.024 and P =0.002,respectively),while that between borderline tumor group and benign tumor group had no statistical difference (P =0.428).Among mucous tumors ( 15/27 ),the mean value of p73 expression in carcinoma group (6/7) was higher than that in benign tumor group ( 1/8 ; P =0.032).No statistical difference of p73 expression was seen between the carcinoma group and ovarian borderline tumor group ( 8/12 ) and between the borderline tumor group and benign tumor group (P =0.234,P =0.201,respectively).p73 promotor methylation was found in 8 of 13 cases of carcinomas but at different methylation levels with a mean value of 8.0％.Two of 5 ovarian borderline tumors showed detectable p73 promotor methylation with a mean value of 9.0％.Compared with the borderline tumors,ovarian carcinomas showed a similar p73 methylation level (P ＞ 0.05).The p73 methylation level in ovarian carcinomas was not associated with histological type,pathogenetic type,histological grade and FIGO stage ( all P ＞ 0.05 ).Conclusions Most of ovarian epithelial tumors express p73 protein with mean values higher in ovarian carcinomas than those in the borderline and benign tumors.Ovarian serous carcinomas have the highest expression level of p73. A simple linear correlation does not exist between the promoter methylation and protein expression of p73.
