CAJ | 학술논문

目的应用计算机图像分析评价大鼠胰腺炎模型的病理变化,探讨数字化评价大鼠胰腺炎模型的可行性.方法用5%牛磺胆酸钠胆胰管内注射建模,分别于操作完成后3、6、12、24、48 h处死动物.用数字切片扫描系统及图像分析软件对胰腺的坏死、水肿、出血及炎细胞浸润进行分析及统计.结果处理后48h胰腺的病理变化[炎细胞:(67.00±49.49)个/200倍视野;坏死:(49.86±21.74)%;出血:(14 445.60±6940.35)μm2;水肿:9.58±0.81]较其他时段有显著的加重(P<0.05);图像分析的诊断一致性(k=0.7)优于人工方法(k=0.3).结论用计算机图像分析评价大鼠胰腺炎模型的病理改变具有客观性强、所得数据精准、不同实验方法间可比性强等优点.
목적 응용계산궤도상분석평개대서이선염모형적병리변화,탐토수자화평개대서이선염모형적가행성.방법 용5%우광담산납담이관내주사건모,분별우조작완성후3、6、12、24、48 h처사동물.용수자절편소묘계통급도상분석연건대이선적배사、수종、출혈급염세포침윤진행분석급통계.결과 처리후48h이선적병리변화[염세포:(67.00±49.49)개/200배시야;배사:(49.86±21.74)%;출혈:(14 445.60±6940.35)μm2;수종:9.58±0.81]교기타시단유현저적가중(P<0.05);도상분석적진단일치성(k=0.7)우우인공방법(k=0.3).결론 용계산궤도상분석평개대서이선염모형적병리개변구유객관성강、소득수거정준、불동실험방법간가비성강등우점.
Objective To apply computer digital image analysis to evaluate pathologic changes in rat model with acute pancreatitis, and explore digitalization of the evaluating process. Methods The model was established by injection of 5% sodium taurocholate into the pancreatic duct of Sprague-Dawley rats. Rats were killed 3, 6, 12, 24, and 48 h after the surgery respectively. Paraffin-embedded sections were made and scanned by Virtual Microscopy system. Digital image analysis was applied to analyze necrosis, edema,hemorrhage and infiltration of inflammatory cells in pancreatic tissues. Results As compared with other groups, the pathologic changes of pancreatitis were significantly more severe in 48-h group [inflammatory cell number: (67.00 ±49.49)/200 × field;necrosis: (49.86±21.74)%;hemorrhage: (14 445. 60 ±6940. 35) μm2;edema: 9.58 ±0. 81;P<0. 05];Cohen's kappa statistics showed that digital image analysis had better inter-rater agreement than Schmidt' s evaluation (Kappa coefficient;0. 7vs0. 3). Conclusion Digital image analysis could improve the objectivity, accuracy, and inter-rater agreement in evaluating pathologic changes in rat model with acute pancreatitis.