中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2012年
6期
421-425
,共5页
周思超%李涛%周海涵%程璇%张乾%谭杰%李承晏%段晏文
週思超%李濤%週海涵%程璇%張乾%譚傑%李承晏%段晏文
주사초%리도%주해함%정선%장건%담걸%리승안%단안문
电刺激%脑梗死%尼氏体%生长相关蛋白-43
電刺激%腦梗死%尼氏體%生長相關蛋白-43
전자격%뇌경사%니씨체%생장상관단백-43
Electrical stimulation%Cerebral infarction%Nissl bodies%Growth-associated protein 43
目的 观察植入式皮质电刺激对脑梗死后尼氏体和生长相关蛋白-43(GAP-43)表达的影响,探讨植入式皮质电刺激治疗脑梗死的机制.方法 制作雄性SD大鼠大脑中动脉梗死(MCAO)模型,选取磁共振成像(MRI)示皮质梗死的大鼠23只,按随机数字表法分为皮质电刺激组(13只)和无电刺激组(共10只).皮质电刺激组植入电刺激器后连续刺激14d,无电刺激组植入电刺激器但不进行电刺激.6周后,取皮质电刺激组梗死灶周边电刺激部位(无电刺激组取相同部位)进行尼氏染色和GAP-43的免疫组织化学染色,用image-Pro Plus 6.0版图像系统进行分析,测量尼氏体占图片总面积的比例,以及GAP-43的阳性表达面积占图片总面积的比例.结果 在梗死灶周围,皮质电刺激组尼氏体着色较深,其尼氏体所占比例为(9.7±2.3)％,高于无电刺激组的(5.9±2.0)％,差异有统计学意义(P＜0.01)；皮质电刺激组GAP-43的阳性表达区域着色较深,其GAP-43的阳性区域比例位(19.3±2.9)％,明显高于无电刺激组的(15.8±3.4)％,差异有统计学意义(P＜0.05).结论 植入式皮质电刺激可增加脑梗死灶周边尼氏体和GAP-43的表达,提示植入式皮质电刺激可通过促进脑梗死灶周围神经元内尼氏体功能的恢复,从而促进轴突生长,重建神经网络,并改善神经缺损功能.
目的 觀察植入式皮質電刺激對腦梗死後尼氏體和生長相關蛋白-43(GAP-43)錶達的影響,探討植入式皮質電刺激治療腦梗死的機製.方法 製作雄性SD大鼠大腦中動脈梗死(MCAO)模型,選取磁共振成像(MRI)示皮質梗死的大鼠23隻,按隨機數字錶法分為皮質電刺激組(13隻)和無電刺激組(共10隻).皮質電刺激組植入電刺激器後連續刺激14d,無電刺激組植入電刺激器但不進行電刺激.6週後,取皮質電刺激組梗死竈週邊電刺激部位(無電刺激組取相同部位)進行尼氏染色和GAP-43的免疫組織化學染色,用image-Pro Plus 6.0版圖像繫統進行分析,測量尼氏體佔圖片總麵積的比例,以及GAP-43的暘性錶達麵積佔圖片總麵積的比例.結果 在梗死竈週圍,皮質電刺激組尼氏體著色較深,其尼氏體所佔比例為(9.7±2.3)％,高于無電刺激組的(5.9±2.0)％,差異有統計學意義(P＜0.01)；皮質電刺激組GAP-43的暘性錶達區域著色較深,其GAP-43的暘性區域比例位(19.3±2.9)％,明顯高于無電刺激組的(15.8±3.4)％,差異有統計學意義(P＜0.05).結論 植入式皮質電刺激可增加腦梗死竈週邊尼氏體和GAP-43的錶達,提示植入式皮質電刺激可通過促進腦梗死竈週圍神經元內尼氏體功能的恢複,從而促進軸突生長,重建神經網絡,併改善神經缺損功能.
목적 관찰식입식피질전자격대뇌경사후니씨체화생장상관단백-43(GAP-43)표체적영향,탐토식입식피질전자격치료뇌경사적궤제.방법 제작웅성SD대서대뇌중동맥경사(MCAO)모형,선취자공진성상(MRI)시피질경사적대서23지,안수궤수자표법분위피질전자격조(13지)화무전자격조(공10지).피질전자격조식입전자격기후련속자격14d,무전자격조식입전자격기단불진행전자격.6주후,취피질전자격조경사조주변전자격부위(무전자격조취상동부위)진행니씨염색화GAP-43적면역조직화학염색,용image-Pro Plus 6.0판도상계통진행분석,측량니씨체점도편총면적적비례,이급GAP-43적양성표체면적점도편총면적적비례.결과 재경사조주위,피질전자격조니씨체착색교심,기니씨체소점비례위(9.7±2.3)％,고우무전자격조적(5.9±2.0)％,차이유통계학의의(P＜0.01)；피질전자격조GAP-43적양성표체구역착색교심,기GAP-43적양성구역비례위(19.3±2.9)％,명현고우무전자격조적(15.8±3.4)％,차이유통계학의의(P＜0.05).결론 식입식피질전자격가증가뇌경사조주변니씨체화GAP-43적표체,제시식입식피질전자격가통과촉진뇌경사조주위신경원내니씨체공능적회복,종이촉진축돌생장,중건신경망락,병개선신경결손공능.
Objective To investigate the effect of implanted cortical electrical stimulation (CES) on the expression of Nissl bodies and growth-associated protein 43 (GAP-43) in the brain after ischemic injury,and its mechanism.Methods Models of middle cerebral artery occlusion (MCAO) were established in 23 male Sprague-Dawley rats.They were randomly divided into a CES group (CES,n=13) and a no stimulation group (NS,n=10) and electrical stimulators were implanted in both groups.CES was applied for 14 d in the CES group but not in the NS group.The expression of Nissl bodies and GAP-43 around the infarct were quantified using version 6.0 of the ImagePro Plus system.Results In the CES group the Nissl bodies had a deep color,and their percentage of area was higher than that in the NS group.The GAP-43 positive expression area also had a relatively deep color,and the average percentage of positive expression area was also higher than that in the NS group.Conclusions CES can enhance the expression of Nissl bodies and GAP-43 after cerebral infarction.This suggests that CES can promote axon growth and the formation of new neural circuits.
