CAJ | 학술논문

目的研究中药淫羊藿的黄酮类成分(FE)预防绝经后骨质疏松的作用是否通过促进肠钙吸收途径.方法 12月龄雌性Wistar大鼠45只,随机分为假手术组和卵巢切除组,即OVX组(卵巢切除+溶媒)、FE组(卵巢切除+淫羊藿黄酮类成分)、CS组(卵巢切除+钙剂+溶媒)和FE+CS组(卵巢切除+淫羊藿黄酮类成分+钙剂).卵巢切除4 d后,给药组开始灌喂,FE和(或)CS,连续用药12周.处死动物留取尿液和血清分别测定肠钙吸收指标,钙代谢自稳调节激素和骨转换标志物.截取左侧股骨近端,分别测定骨强度、骨材料-结构参数(包括肢体定量CT测定的体积骨密度、骨截面分布和显微CT定量测定的骨小梁三维结构)以及肢体定量CT定义的骨表面参数.结果 FE能显著预防大鼠去势引起的骨强度和材料结构的退化,对去势后子宫重量的改变没有影响,OVX组为(572±23)g,FE组为564 g,P＞0.05.钙剂(CS组)不能预防去势导致的骨强度的下降.FE和CS之间的析因分析结果表明两者联合应用能够分别在促进肠钙吸收和抑制骨吸收方面产生协同增强作用,但在抑制甲状旁腺激素方面没有协同效应;FE和CS在骨形成参数和骨材料-结构-强度参数方面也并没有体现协同增强作用.结论中药淫羊霍的黄酮类成分能有效地预防去势大鼠骨质疏松,且不刺激子宫增生,其抑制骨吸收和促进骨形成的作用机制并非依赖肠钙吸收途径.
목적 연구중약음양곽적황동류성분(FE)예방절경후골질소송적작용시부통과촉진장개흡수도경.방법 12월령자성Wistar대서45지,수궤분위가수술조화란소절제조,즉OVX조(란소절제+용매)、FE조(란소절제+음양곽황동류성분)、CS조(란소절제+개제+용매)화FE+CS조(란소절제+음양곽황동류성분+개제).란소절제4 d후,급약조개시관위,FE화(혹)CS,련속용약12주.처사동물류취뇨액화혈청분별측정장개흡수지표,개대사자은조절격소화골전환표지물.절취좌측고골근단,분별측정골강도、골재료-결구삼수(포괄지체정량CT측정적체적골밀도、골절면분포화현미CT정량측정적골소량삼유결구)이급지체정량CT정의적골표면삼수.결과 FE능현저예방대서거세인기적골강도화재료결구적퇴화,대거세후자궁중량적개변몰유영향,OVX조위(572±23)g,FE조위564 g,P＞0.05.개제(CS조)불능예방거세도치적골강도적하강.FE화CS지간적석인분석결과표명량자연합응용능구분별재촉진장개흡수화억제골흡수방면산생협동증강작용,단재억제갑상방선격소방면몰유협동효응;FE화CS재골형성삼수화골재료-결구-강도삼수방면야병몰유체현협동증강작용.결론 중약음양곽적황동류성분능유효지예방거세대서골질소송,차불자격자궁증생,기억제골흡수화촉진골형성적작용궤제병비의뢰장개흡수도경.
Objective To test our hypothesis whether a group of flavonoids (FE) derived from herbal Epimedium exerted its prevention of estrogen-deficiency-induced osteoporosis mainly through an enhancement in intestinal calcium absorption. Methods Forty-five 12-month-old female Wistar rats were randomly assigned into one sham-operated group and four ovariectomy (OVX) subgroups, i.e. OVX with vehicle (OVX group), OVX with FE (FE group), OVX with calcium supplement (CS group), and OVX with FE and CS. Daily oral administration of FE (10 mg·kg-1·d-1) and/or CS (56 mg·kg-1·d-1)started on day 4 after OVX for 12 weeks. Before sacrificing the animals, urine and serum samples were collected for assaying indicators for intestinal calcium absorption, regulator of calcium homeostasis and markers for bone turnover. Then, the left proximal femur was dissected for the primary-end-point index(failure force) , and the second-end-point indexes (pQCT-calculated densitometry, geometry and micro-CT-quantified 3-D trabecula mictroarchitecture), as well as pQCT-defined cross-sectional envelope. Results FE prevented OVX-induced deterioration in failure force as well as the second-end-point indexes with no increase in uterus weight. CS had no prevention effect on OVX-induced reduction in failure force. Two-way factorial interaction analysis between FE and CS showed that the un-enhanced suppression of parathyroid hormone for calcium homeostasis provided no link between the enhanced intestinal calcium absorption and the enhanced inhibition of bone resorption in the present study. Furthermore, discrepancy between the enhanced intestinal calcium absorption and the un-enhanced primary / second-end-point indexes as well as anabolic effect was also found by the interaction analysis. Conclusion Independent of intestinal calcium absorption,FE inhibited bone resorption, stimulated bone formation and accordingly prevented osteoporosis without hyperplastic effect on uterus in the OVX rat model.