中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
3期
539-540
,共2页
潘延凤%娄海山%余祖江%张贤强%冯磊%梁红霞
潘延鳳%婁海山%餘祖江%張賢彊%馮磊%樑紅霞
반연봉%루해산%여조강%장현강%풍뢰%량홍하
肝细胞癌%长链非编码RNA%UC001kfo%肿瘤转移
肝細胞癌%長鏈非編碼RNA%UC001kfo%腫瘤轉移
간세포암%장련비편마RNA%UC001kfo%종류전이
Hepatocellular carcinoma%Long non-coding RNA%UC001kfo%Tumor metastasis
目的 探讨新的长链非编码RNA(lncRNA) UC001 kfo在肝癌中的表达及与肝癌侵袭、转移的关系.方法 收集肝脏组织60例,分为肝硬化组、肝癌组、癌旁组、门静脉转移肝癌组.原位杂交检测UC001 kfo和ACTA2(α-平滑肌肌动蛋白的基因)在肝癌组织的表达.结果 UC001kfo在肝硬化组不表达或低表达,在其他3组均为阳性表达,以上4组的表达值分别为113.30±11.79、137.59±6.23、148.78±8.23、160.28 ±9.47,方差分析显示UC001kfo表达差异有统计学意义(P<0.01),4组间的差异有统计学意义(P<0.01),分别为肝硬化组<癌旁组织<肝癌组<门脉癌栓组.ACTA2的表达分别为109.89±9.74、125.22±32.16、149.06±8.43、156.57±8.86,ACTA2表达差异有统计学意义(P<0.01),4组间差异有统计学意义(P<0.05),表达分别为肝硬化组<癌旁组织<肝癌组<门脉癌栓组.结论 UC001 kfo和ACTA2在肝癌中表达明显增加,特别是门静脉癌栓组,UC001kfo可能通过调控ACTA2的表达促进肝癌的侵袭转移.
目的 探討新的長鏈非編碼RNA(lncRNA) UC001 kfo在肝癌中的錶達及與肝癌侵襲、轉移的關繫.方法 收集肝髒組織60例,分為肝硬化組、肝癌組、癌徬組、門靜脈轉移肝癌組.原位雜交檢測UC001 kfo和ACTA2(α-平滑肌肌動蛋白的基因)在肝癌組織的錶達.結果 UC001kfo在肝硬化組不錶達或低錶達,在其他3組均為暘性錶達,以上4組的錶達值分彆為113.30±11.79、137.59±6.23、148.78±8.23、160.28 ±9.47,方差分析顯示UC001kfo錶達差異有統計學意義(P<0.01),4組間的差異有統計學意義(P<0.01),分彆為肝硬化組<癌徬組織<肝癌組<門脈癌栓組.ACTA2的錶達分彆為109.89±9.74、125.22±32.16、149.06±8.43、156.57±8.86,ACTA2錶達差異有統計學意義(P<0.01),4組間差異有統計學意義(P<0.05),錶達分彆為肝硬化組<癌徬組織<肝癌組<門脈癌栓組.結論 UC001 kfo和ACTA2在肝癌中錶達明顯增加,特彆是門靜脈癌栓組,UC001kfo可能通過調控ACTA2的錶達促進肝癌的侵襲轉移.
목적 탐토신적장련비편마RNA(lncRNA) UC001 kfo재간암중적표체급여간암침습、전이적관계.방법 수집간장조직60례,분위간경화조、간암조、암방조、문정맥전이간암조.원위잡교검측UC001 kfo화ACTA2(α-평활기기동단백적기인)재간암조직적표체.결과 UC001kfo재간경화조불표체혹저표체,재기타3조균위양성표체,이상4조적표체치분별위113.30±11.79、137.59±6.23、148.78±8.23、160.28 ±9.47,방차분석현시UC001kfo표체차이유통계학의의(P<0.01),4조간적차이유통계학의의(P<0.01),분별위간경화조<암방조직<간암조<문맥암전조.ACTA2적표체분별위109.89±9.74、125.22±32.16、149.06±8.43、156.57±8.86,ACTA2표체차이유통계학의의(P<0.01),4조간차이유통계학의의(P<0.05),표체분별위간경화조<암방조직<간암조<문맥암전조.결론 UC001 kfo화ACTA2재간암중표체명현증가,특별시문정맥암전조,UC001kfo가능통과조공ACTA2적표체촉진간암적침습전이.
Objective Investigate the expression of a new long non-coding RNA (IncRNA) UC001kfo in liver cancer and its relations to the invasion and metastasis of liver cancer.Methods Collect 10 cases of liver cirrhosis tissue samples,25 cases of liver cancer tissue samples,25 cases of samples of the tissues near the cancer and 10 cases of samples of the tissues of portal vein transferred liver cancer.The in-situ hybridization method is used to test the expression of UC001kfo and ACTA2 ( gene of α-smooth muscle actin) in liver cancer tissues.Results Results of the in-situ hybridization indicate that UC001kfo is not expressed or expressed in low quantity in the liver cirrhosis group,but it is expressed positively in all the liver cancer tissues,tissues near the cancer and portal vein transferred liver cancer tissues.The expression values of the above four groups are respectively 113.30 ± 11.79,137.59 ±6.23,148.78 ±8.23 and 160.28 ±9.47.Variance analysis shows that the differences of UC00lkfo expression have statistically significance ( P < 0.01 ).The differences of four groups have statistically significance ( P < 0.01 ),which can be arrayed as:liver cirrhosis group < tissues near the cancer < liver cancer group < portal vein cancer embolus group.The ACTA2 values of the above four groups are respectively 109.89 ± 9.74,125.22 ± 32.16,149.06 ±8.43 and 156.57 ±8.86.Variance analysis shows that the differences of ACTA2 expression have statistically significance (P <0.01 ).The differences of four groups have statistically significance (P < 0.05) which can be arrayed as:liver cirrhosis group < tissues near the cancer < liver cancer group < portal vein cancer embolus group.Conclusion Expression of Uc001kfo and ACTA2 are obviously increased in liver cancer,especially the portal vein liver cancer embolus group.UC001kfo may have participated in the occurrence of liver cancer by regulating and controlling the expression of ACTA2 and is related to the invasion and metastasis of liver cancer.